A Complex Case of Aspergillus Infection of the Brain and Its Future Medical Implications

Janardan, Abhishek; Prokhoda, Polina; Razzak, Abrahim N; Jethwa, Trisha; Paudel, Hari R

doi:10.7759/cureus.29756

Cited by 2 publications

(1 citation statement)

References 12 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This infection spreads brain and cause lesions in a parenchyma of the brain due to defective cellular immunity. The brain lesions were secondarily resulted in fungal blood spread that reaches the brain [25]. Contextually, study demonstrated the brain of a patient infected with A. flavus.…”

Section: Discussionmentioning

confidence: 99%

Infection of Aspergillus flavus in Brain Tissues of Albino Male Rats

Alhasan

2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

spergillus flavus is considered a one of the fungi which can cause dangerous fungal infections for humans and animals. In this study, an isolate of this fungus was identified according to the morphological and molecular methods. The polymerase chain reaction ( PCR ) was used to confirm the identification of the isolate appeared genes of the A. flavus ( FLA ) and a putative RNA-Pol II transcription elongation factor ( RtfA) which located in 497 and 263 base pairs ( bp), respectively. Experimentally, two groups of the albino male rats were used in this study by which one group was immunosuppressed and infected with 0.5 ml containing 10 6 spores of A. flavus intraperitoneally while other group was inoculated with 0.5 ml of the sterile normal saline. Brains of the infected rats revealed lesions that the brain appeared swelling and congestion of the cerebrum pushing the cerebellum down. Cerebral cortex with prominent haemorrhage was illustrated with lymphocytic infiltration. Also, demonstrating pyogenic granuloma in the cerebral cortex composed of centre of caseous necrosis surrounded by massive neutrophils and other chronic inflammatory cells. In addition, the brain sections possessed multiple multinucleated giant cells and dissemination of A. flavus into ventricular space and choroid plexus.

show abstract

Section: Discussionmentioning

confidence: 99%

Infection of Aspergillus flavus in Brain Tissues of Albino Male Rats

Alhasan

2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

Comparison of the BluePoint MoldID oligonucleotide array and Bruker Biotyper MALDI-TOF MS for the identification of filamentous fungi

Chou,

Chao,

et al. 2024

J Clin Microbiol

View full text Add to dashboard Cite

BluePoint MoldID can identify 43 fungal species through nucleic acid array hybridization and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) can identify 247 filamentous fungi through mass spectrometry. First, 43 standard isolates from the Bioresource Collection and Research Center, Taiwan, and the College of American Pathologists and 41 clinical Aspergillus species isolates confirmed by rDNA-ITS sequencing were analyzed using BluePoint MoldID and Bruker MALDI-TOF MS. BluePoint MoldID accurately identified 79% (34/43) of the standard isolates to the species level but failed to recognize nine isolates absent from its database; additionally, 87.8% (36/41) of the clinical isolates were identified at the species level, with 80.5% (33/41) accuracy. MALDI-TOF MS identified 86% (37/43) of the standard isolates, with 81.4% (35/43) accurately identified at the species level and two at the genus level, and identified all the clinical isolates, with 92.6% (38/41) accurately identified at the species level. Next, we analyzed 93 clinical Aspergillus species and compared the results by rDNA-ITS sequencing. BluePoint MoldID identified 87.1% (81/93) of the isolates at the species level, with 80.6% (75/93) accuracy. MALDI-TOF MS identified 97.8% (91/93) of the isolates, including some uncommon species, with 90.3% (84/93) accuracy at the species level. BluePoint MoldID and MALDI-TOF MS had turnaround times of 8 and 2 h, respectively, significantly reducing the time needed to identify filamentous fungi. IMPORTANCE The BluePoint MoldID kit is an oligonucleotide array used for the identification of filamentous fungi, and it has not yet been mentioned in recent studies. We used a BluePoint MoldID kit to identify standard and clinical filamentous fungal isolates and compared its performance with that of Bruker MALDI-TOF MS. The former accurately identified 80.2% of the isolates (142/177), and the latter identified 92.6% of the isolates (164/177). The performance of the BluePoint MoldID kit was slightly inferior to that of Bruker MALDI-TOF MS because of the smaller database. However, the BluePoint MoldID kit can cover most clinically common opportunistic fungal infections; thus, it offers an alternative method for laboratories that lack MALDI-TOF MS equipment, as the device is less expensive.

show abstract

A Complex Case of Aspergillus Infection of the Brain and Its Future Medical Implications

Cited by 2 publications

References 12 publications

Infection of Aspergillus flavus in Brain Tissues of Albino Male Rats

Infection of Aspergillus flavus in Brain Tissues of Albino Male Rats

Comparison of the BluePoint MoldID oligonucleotide array and Bruker Biotyper MALDI-TOF MS for the identification of filamentous fungi

Contact Info

Product

Resources

About