A complex signaling trio in seed germination: Auxin-JA-ABA

Sybilska, Ewa; Daszkowska‐Golec, Agata

doi:10.1016/j.tplants.2023.05.003

Cited by 14 publications

(5 citation statements)

References 12 publications

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“…The RC may possess more sensitive JA receptors or more effective signal transduction components, thereby exhibiting higher resistance to PWN invasion ( Figure 1A ). It is worth noting that the JA signaling pathway does not exist in isolation but interacts with other plant hormones (such as auxin and abscisic acid) and environmental signals (such as light and temperature) to form a complex regulatory network (Qiu et al, 2022; Hu et al, 2023b; Sybilska and Daszkowska-Golec, 2023). Different pines may exhibit different regulatory interactions that affect their defense mechanisms.…”

Section: Discussionmentioning

confidence: 99%

The molecular basis of pine wilt disease resistance inPinus massoniana

Wang,

Cai,

Zeng

et al. 2024

Preprint

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Pine wilt disease (PWD), caused by the pine wood nematode (PWN)Bursaphelenchus xylophilus, results in significant economic and ecological damage toPinusforests and plantations worldwide.Pinus massonianais the primary host of PWD in southern China, but its response to the PWN remains largely unstudied. Previously, we observed PWD in aP. massoniananursery that contained over 71 commonly used cultivars. Through field phenotyping, we identified two groups of PWN resistant cultivars. Resistant cultivars (RC) exhibited very low PWN carrying amounts (PCA) and had relatively low mortality, and tolerant cultivars (TC) had high PCA but low mortality. In this study, we confirmed via PWN inoculation assays that the resistant and tolerant cultivars had lower mortality rates, 10% and 11%, respectively, than other cultivars (which had a mortality rate of 83.3%). The RC had a PCA that was significantly lower than that of other cultivars, while the TC exhibited a higher PCA. To explore the molecular mechanisms underlying the response ofP. massonianato PWN, the transcriptome and metabolome of the above cultivars were profiled by high throughput sequencing. As no reference genome is available forP. massoniana, we generated a new full-length transcriptome library with iso-seq. Using the transcriptome and metabolome from differentP. massonianacultivars inoculated with PWN, we found three major PWD resistance strategies. 1) The common response strategy involved three important molecular pathways. First, synthesis of indole-3-acetic acid (IAA) and abcisic acid (ABA) were suppressed during PWN invasion, thus suppressing the synthesis of polysaccharides (especially myo-inositol and trehalose) and (-)-riboflavin through ABC transporters. Second, by inhibiting aspartic acid, the synthesis of arginine and proline through APS5 (aspartate aminotransferase 5) were suppressed. Third, by reducing cysteine, GERD (germacrene D synthase) was up-regulated, and sesquiterpenoid and triterpenoid metabolites were accumulated to resist PWN invasion. 2) The most effective resistance strategy involved the accumulation of reactive oxygen species (ROS), which enhanced jasmonic acid (JA) accumulation and highly induced the expression of chitinase, thus improving resistance to PWN. 3) The tolerance strategy involved the induction of phosphatidylcholine, which promoted flavonoid and anthocyanin synthesis via LPIN (phosphatidate phosphatase), LOX2S (lipoxygenase), and LOX1_5 (linoleate 9S-lipoxygenase), thus significantly inhibiting the pathogenicity of the PWN but not the PCA. These results illustrate the molecular mechanisms by whichP. massonianacultivars resist or tolerate PWN and are of great significance in the prevention and control of PWD.

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Section: Discussionmentioning

confidence: 99%

The molecular basis of pine wilt disease resistance inPinus massoniana

Wang,

Cai,

Zeng

et al. 2024

Preprint

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“…The phytohormone auxin and JA play essential regulatory roles in multiple aspects of plant growth and development and in stress responses [ 27 , 28 , 29 ]. The YUCCA and TAR members are key enzymes of the IAA-biosynthetic pathway [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…StARF16 regulates defense gene StNPR1 during the JA-mediated defense response upon necrotrophic pathogen interaction [34]. The GH3 genes are cytosolic, acidic amido synthetases of the firefly luciferase group that conjugate auxins, jasmonates, and benzoate derivatives to a wide group The phytohormone auxin and JA play essential regulatory roles in multiple aspects of plant growth and development and in stress responses [27][28][29]. The YUCCA and TAR members are key enzymes of the IAA-biosynthetic pathway [30,31].…”

Section: Ibsaur36 Is Animportant Factor In Auxin and Ja Signaling Pat...mentioning

confidence: 99%

A Small Auxin-Up RNA Gene, IbSAUR36, Regulates Adventitious Root Development in Transgenic Sweet Potato

Zhou,

Li,

et al. 2024

Genes

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Small auxin-upregulated RNAs (SAURs), as the largest family of early auxin-responsive genes, play important roles in plant growth and development processes, such as auxin signaling and transport, hypocotyl development, and tolerance to environmental stresses. However, the functions of few SAUR genes are known in the root development of sweet potatoes. In this study, an IbSAUR36 gene was cloned and functionally analyzed. The IbSAUR36 protein was localized to the nucleus and plasma membrane. The transcriptional level of this gene was significantly higher in the pencil root and leaf.This gene was strongly induced by indole-3-acetic acid (IAA), but it was downregulated under methyl-jasmonate(MeJA) treatment. The promoter of IbSAUR36 contained the core cis-elements for phytohormone responsiveness. Promoter β-glucuronidase (GUS) analysis in Arabidopsis showed that IbSAUR36 is highly expressed in the young tissues of plants, such as young leaves, roots, and buds. IbSAUR36-overexpressing sweet potato roots were obtained by an efficient Agrobacterium rhizogenes-mediated root transgenic system. We demonstrated that overexpression of IbSAUR36 promoted the accumulation of IAA, upregulated the genes encoding IAA synthesis and its signaling pathways, and downregulated the genes encoding lignin synthesis and JA signaling pathways. Taken together, these results show that IbSAUR36 plays an important role in adventitious root (AR) development by regulating IAA signaling, lignin synthesis, and JA signaling pathways in transgenic sweet potatoes.

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“…The balance of these hormones is critical for regulating seed dormancy and the transition to germination. The complicated interaction of auxin with ABA and GAs aids in the fine-tuning of germination time and circumstances (Del Bel et al, 2023) temperature, and moisture levels can influence auxin levels and signaling pathways, altering seed dormancy and germination potential (Sybilska & Daszkowska-Golec, 2023).…”

Section: Media Composition (Podzolic Soil) and Seed Dormancy Auxinmentioning

confidence: 99%

An Utilizing Marginal Red Yellow Podzolic Soil as a Growing Medium for Auxin-Soaked Cutting Propagation of Red Master Grapes (Vitis vinifera L)

Pamungkas,

Pramudya,

Sukmawati

et al. 2023

J. appl. agricultural sci. technol.

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Grape (Vitis vinifera) cultivation is hampered by a lack of suitable land and challenging nurseries. Use of crimson yellow podzolic soil for vine cuttings can be suboptimal. Although podzolic soil has a low capacity to absorb macro- and micronutrients and an acidic pH, it can nevertheless be used as a growing medium. A synthetic growth regulator (ZPT) bath of naphthalene acetic acid (NAA) needs to be added to the local red master grape variety, which has started to develop as a scion in nurseries. In order to assess the impacts of NAA-soaked concentrations on podzolic soil media and identify the morphological traits of local Red Master grape cuttings, this study set out to identify these traits. Its goal was to find out how auxin affected the development and yield of grapes (Vitis vinifera L.) grown in red yellow podzolic soil. A non-factorial totally randomized design with one factor (NAA concentration) was used in the study, with a block of 4 treatments and 3 repetitions. The varied NAA concentrations used in the treatment procedure included F0 (0 g L-1), F1 (2 g L-1), F2 (4 g L-1), and F3 (6 g L-1). The variables that were observed included the number of shoot bursts, the timing of leaf emergence, the number of sheet leaves, the length of the tendrils, and the proportion of live cuttings. ANOVA was used to tabulate and analyze observational data at a 5% level, and DMRT analysis was used to continue the analysis at the same level. According to the results, soaked NAA at a concentration of 0 g L-1 (S0) as the control treatment had the best results for the variable number of shoots and leaves, while a concentration of 2 g L-1 (S1) had the best results for the variable length of tendrils. However, soaked NAA had no significant impact on the variable when leaves emerged. Soaking auxin at the bottom of cuttings had no effect on shoot formation. Reduction of auxin due to defoliation can result in the expression of the isopentenyl-transferase (IPT) gene. Auxins, such as Indole-3-acetic acid (NAA), are usually involved in the regulation of root and leaf growth. If a plant has many leaves and few roots without the use of additional NAA or auxin, several factors may play a role, such as plant genetics, environmental conditions, and environmental stress. The use of additional NAA or auxin can explicitly affect the growth of roots and leaves. The survival percentage of cuttings is still low because it is influenced by the quality (material) of the cuttings, the age of the parent tree, growing media and water availability.

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A complex signaling trio in seed germination: Auxin-JA-ABA

Cited by 14 publications

References 12 publications

The molecular basis of pine wilt disease resistance inPinus massoniana

The molecular basis of pine wilt disease resistance inPinus massoniana

A Small Auxin-Up RNA Gene, IbSAUR36, Regulates Adventitious Root Development in Transgenic Sweet Potato

An Utilizing Marginal Red Yellow Podzolic Soil as a Growing Medium for Auxin-Soaked Cutting Propagation of Red Master Grapes (Vitis vinifera L)

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