A Comprehensive and Rapid Quality Evaluation Method of Traditional Chinese Medicine Decoction by Integrating UPLC-QTOF-MS and UFLC-QQQ-MS and Its Application

Abstract

Decoction is one of the oldest forms of traditional Chinese medicine and it is widely used in clinical practice. However, the quality evaluation and control of traditional decoction is a challenge due to the characteristics of complicated constituents, water as solvent, and temporary preparation. ShenFu Prescription Decoction (SFPD) is a classical prescription for preventing and treating many types of cardiovascular disease. In this article, a comprehensive and rapid method for quality evaluation and control of SFPD was developed, via qualitative and quantitative analysis of the major components by integrating ultra-high-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry and ultra-fast-performance liquid chromatography equipped with triple quadrupole mass spectrometry. Consequently, a total of 39 constituents were tentatively identified in qualitative analysis, of which 21 compounds were unambiguously confirmed by comparing with reference substances. We determined 13 important constituents within 7 min by multiple reaction monitoring. The validated method was applied for determining five different proportion SFPDs. It was found that different proportions generated great influence on the dissolution of constituents. This may be one of the mechanisms for which different proportions play different synergistic effects. Therefore, the developed method is a fast and useful approach for quality evaluation of SFPD.

Jinlian Xiaodu Decoction Protects against Bleomycin-Induced Pulmonary Fibrosis in Rats

“…JXD contains Anoectochilus (5 g), Qiliang (20 g), and Polygonum cuspidatum (15 g), which were obtained from the same lot in the Department of Pharmacy in the Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua, China) after identification by a pharmacist. Subsequently, the water decoction was prepared by the traditional decoction method [ 21 ]. First, Anoectochilus , Qiliang, and P. cuspidatum were mixed at a ratio of 1 : 4 : 3 followed by macerating in distilled water for 60 min at room temperature.…”

Research Progress of Male Reproductive Toxicity of Chinese Materia Medicas

“…Papaya alkaloids [46] can block calcium channels in nerve tissue, inhibit calcium influx and reduce the release of pain mediators such as prostaglandins. However, the alkaloids in Carica papaya L [47] can also inhibit the activity of steroidal enzymes in rats' testes. Both oral intake and intramuscular injection of this kind of alkaloids can lead to reversible sterility in rats.…”

Chemical Characterization and Metabolic Profiling of the Compounds in the Chinese Herbal Formula Li Chang Decoction by UPLC-QTOF/MS

“…Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF/MS) provides a rapid and reliable method to identify the component of natural medicine, which promotes the development of natural medicine component analysis and new drug discovery [ 9 , 10 ]. Herein, we recruited an UPLC-QTOF/MS method to profile the effective components of LCD, and the unknown components were classified and assigned based on the fragmentation patterns and diagnostic ions of different structural types of components.…”