A comprehensive research on Lactone Sophorolipid (LSL) and Soy Protein Isolate (SPI) interacting mixture

Interactional, functional, and biological properties of lactone sophorolipid (LSL) and collagen oligopeptides (COP) in aqueous solution

“…The ST measurements were determined according to published methods. [22] This method can be used to check the absence of surface-active contaminants in the buffer if the measured value of surface tension wa 72.0 ± 0.5 mN/m at 298.15 K. A stock mixed solution of COP and LSL with a concentration of 10 g/L and a different ratio was prepared in PBS buffer solution and then the solution was diluted using PBS to prepare a series of the desired solutions.…”

“…For the details of the experiment, please refer to the previous publications. [22] The preparation of the solution was same as described in 'Surface tension measurement'.…”

“…The foaming properties were determined according to the oscillometric method. [22,23] The preparation of the solution was same as described in 'Surface tension measurement', then the initial liquid level height (H), the initial foam height (H 0 ) and the foam height falling over time (H 1 ) were recorded during the attenuation process. Foam capacity (FC) and stability (FS) were calculated using the following Equations ( 2) and (3):…”

“…The difference between the emission wavelength and the excitation wavelength of the synchronous fluorescence spectroscopy was fixed at 15 nm and 60 nm, respectively. Please refer to previous publications [22] for the other details of the experiment.…”

“…Therefore we investigated the effects of different ratios of COP/LSL on its physical and chemical properties, and the interaction forces and mechanisms between COP and LSL were studied using spectral analysis. Combined with previous studies on the LSL and soy protein isolate (SPI) system, [22] it is expected to expand its application and offer a theory basis for biosurfactant in the food and cosmetic industries.…”