A comprehensive review on 3D tissue models: Biofabrication technologies and preclinical applications

Xie, Renjian; Pal, Vaibhav; Yu, Yanrong; Lu, Xiaolu; Gao, Mengwei; Liang, Shijie; Huang, Miao; Peng, Weijie; Ozbolat, Ibrahim T.

doi:10.1016/j.biomaterials.2023.122408

Cited by 14 publications

(3 citation statements)

References 276 publications

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“…Biofabrication approaches have progressed considerably in the last decade, with the primary goal of recapitulating the key structure and performance of native human tissues. [20] However, to date it has remained virtually impossible to fabricate tissue constructs that display the exact same organization of individual cells in their native cellular microenvironments. Spheroids/organoids rely largely on the stochastic self- assembly of aggregates of cells.…”

Section: Introductionmentioning

confidence: 99%

Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution

Helms,

Oyama,

Ware

et al. 2024

Preprint

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Tissue development, function, and disease are largely driven by the spatial organization of individual cells and their cell-cell interactions. Precision engineered tissues with single-cell spatial resolution, therefore, have tremendous potential for next generation disease models, drug discovery, and regenerative therapeutics. Despite significant advancements in biofabrication approaches to improve feature resolution, strategies to fabricate tissues with the exact same organization of individual cells in their native cellular microenvironment have remained virtually non-existent to date. Here we report a method to spatially pattern single cells with up to eight cell phenotypes and subcellular spatial precision. As proof-of-concept we first demonstrate the ability to systematically assess the influence of cellular microenvironments on cell behavior by controllably altering the spatial arrangement of cell types in bioprinted precision cell-cell interaction arrays. We then demonstrate, for the first time, the ability to produce high-fidelity replicas of an annotated cancer patient biopsy with subcellular resolution. The ability to replicate native cellular microenvironments marks a significant advancement for precision biofabricated in-vitro models, where heterogenous tissues can be engineered with single-cell spatial precision to advance our understanding of complex biological systems in a controlled and systematic manner.

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Section: Introductionmentioning

confidence: 99%

Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution

Helms,

Oyama,

Ware

et al. 2024

Preprint

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“…The cell-cell and cell-matrix interactions play crucial role during tissue development and disease. Thus, successful fabrication of a functional tissue requires manufacturing strategies that can recapitulate the native ECM along with precise assembly and patterning of cells into dense structures [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

3D bioprinting of dense cellular structures within hydrogels with spatially controlled heterogeneity

Abaci,

Guvendiren

2024

Biofabrication

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Embedded bioprinting is an emerging technology for precise deposition of cell-laden or cell-only bioinks to construct tissue like structures. Bioink is extruded or transferred into a yield stress hydrogel or a microgel support bath allowing print needle motion during printing and providing temporal support for the printed construct. Although this technology has enabled creation of complex tissue structures, it remains a challenge to develop a support bath with user-defined extracellular mimetic cues and their spatial and temporal control. This is crucial to mimic the dynamic nature of the native tissue to better regenerate tissues and organs. To address this, we present a bioprinting approach involving printing of a photocurable viscous support layer and bioprinting of a cell-only or cell-laden bioink within this viscous layer followed by brief exposure to light to partially crosslink the support layer. This approach does not require shear thinning behavior and is suitable for a wide range of photocurable hydrogels to be used as a support. It enables multi-material printing to spatially control support hydrogel heterogeneity including temporal delivery of bioactive cues (e.g., growth factors), and precise patterning of dense multi-cellular structures within these hydrogel supports. Here, dense stem cell aggregates are printed within methacrylated hyaluronic acid-based hydrogels with patterned heterogeneity to spatially modulate human mesenchymal stem cell osteogenesis. This study has significant impactions on creating tissue interfaces (e.g., osteochondral tissue) in which spatial control of ECM properties for patterned stem cell differentiation is crucial.

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“…Rigorous regulatory and experimental evaluations and preclinical assessments are required before they can be used in the clinic. 44 Before the clinical application and translation of whiskers in dentistry, it is important to ensure that they strictly comply with the relevant medical device regulations and undergo the registration and approval process. 45 Thus, whiskers need to undergo rigorous and exhaustive clinical trials to fully validate their effectiveness in diagnosing and treating oral diseases.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Whiskers: Properties and Clinical Applications in Dentistry

Han,

Zhang,

Liu

et al. 2024

IJN

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Whiskers are nanoscale, high-strength fibrous crystals with a wide range of potential applications in dentistry owing to their unique mechanical, thermal, electrical, and biological properties. They possess high strength, a high modulus of elasticity and good biocompatibility. Hence, adding these crystals to dental composites as reinforcement can considerably improve the mechanical properties and durability of restorations. Additionally, whiskers are involved in inducing the value-added differentiation of osteoblasts, odontogenic osteocytes, and pulp stem cells, and promoting the regeneration of alveolar bone, periodontal tissue, and pulp tissue. They can also enhance the mucosal barrier function, inhibit the proliferation of tumor cells, control inflammation, and aid in cancer prevention. This review comprehensively summarizes the classification, properties, growth mechanisms and preparation methods of whiskers and focuses on their application in dentistry. Due to their unique physicochemical properties, excellent biological properties, and nanoscale characteristics, whiskers show great potential for application in bone, periodontal, and pulp tissue regeneration. Additionally, they can be used to prevent and treat oral cancer and improve medical devices, thus making them a promising new material in dentistry.

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A comprehensive review on 3D tissue models: Biofabrication technologies and preclinical applications

Cited by 14 publications

References 276 publications

Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution

Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution

3D bioprinting of dense cellular structures within hydrogels with spatially controlled heterogeneity

Recent Advances in Whiskers: Properties and Clinical Applications in Dentistry

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