A comprehensive review on mutan (a mixed linkage of α-1-3 and α-1-6 glucans) from bacterial sources

Boddapati, Sirisha; Gummadi, Sathyanarayana N.

doi:10.1080/02648725.2021.2003072

Cited by 5 publications

(3 citation statements)

References 127 publications

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“…Agrobacterium tumefaciens Succinoglycan [26] Gluconacetobacter, Sarcina Agrobacterium, Rhizobium Cellulose [27] Agrobacterium leguminum Curdlan [28] Leuconostoc dextranicum Glucan [29] Leuconostoc, Streptococcus, Weissella, Pediococcus Lactobacillus Dextran [30] Pseudomonas aeruginosa Algins [31] Acinetobacter sp. Emulsan [32] Sphingomonas paucimobilis Gellan [33] Streptococcus equisimilis, S. Pyogenes, S. thermophilus, S. equi Hyaluronic acid [34] Acetobacter, Bacillus, Brenneria, Geobacillus, Halomonas, Lactobacillus, Zymomonas, Saccharomyces Levan [35] Aureobasidium pullulans, Cytaria spp., Teloschistes flavicans, Rhodototula bacarum, Cryphonectria parasitica Pullulan [36] Streptococcus mutans Mutan [37] Xanthomonas spp.…”

Section: Producing Microorganisms Type Of Sugar Produced Referencementioning

confidence: 99%

Gellan Gum as a Unique Microbial Polysaccharide: Its Characteristics, Synthesis, and Current Application Trends

Abdl Aali,

Al-Sahlany

2024

Gels

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Gellan gum (GG) is a linear, negatively charged exopolysaccharide that is biodegradable and non-toxic. When metallic ions are present, a hard and transparent gel is produced, which remains stable at a low pH. It exhibits high water solubility, can be easily bio-fabricated, demonstrates excellent film/hydrogel formation, is biodegradable, and shows biocompatibility. These characteristics render GG a suitable option for use in food, biomedical, and cosmetic fields. Thus, this review paper offers a concise summary of microbial polysaccharides. Moreover, an in-depth investigation of trends in different facets of GG, such as biosynthesis, chemical composition, and physical and chemical properties, is emphasized. In addition, this paper highlights the process of extracting and purifying GG. Furthermore, an in-depth discussion of the advantages and disadvantages of GG concerning other polysaccharides is presented. Moreover, the utilization of GG across different industries, such as food, medicine, pharmaceuticals, cosmetics, etc., is thoroughly examined and will greatly benefit individuals involved in this field who are seeking fresh opportunities for innovative projects in the future.

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Section: Producing Microorganisms Type Of Sugar Produced Referencementioning

confidence: 99%

Gellan Gum as a Unique Microbial Polysaccharide: Its Characteristics, Synthesis, and Current Application Trends

Abdl Aali,

Al-Sahlany

2024

Gels

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“…2 Mutan, a water-insoluble glucan with primarily α-1,3 linkage, gives structural rigidity to the biofilm matrix, while water-soluble dextrans, predominantly with α-1,6 linkage, serve as a carbohydrate reserve and prevent the biofilm from drying. 3,4 For many years, antibacterial chemical agents were used to treat dental plaques and caries. In recent years, usage of microbial enzymes in toothpaste and mouthwashes to reduce dental plaque and maintain oral hygiene has garnered considerable attention.…”

Section: Introductionmentioning

confidence: 99%

“…EPS comprises glucose polymers (mutan and dextran) with α‐1,3 and α‐1,6 linkages, and accounts for 40% of the dry weight of dental plaque 2 . Mutan, a water‐insoluble glucan with primarily α‐1,3 linkage, gives structural rigidity to the biofilm matrix, while water‐soluble dextrans, predominantly with α‐1,6 linkage, serve as a carbohydrate reserve and prevent the biofilm from drying 3,4 …”

Section: Introductionmentioning

confidence: 99%

Production and application of purified mutanase from novel Cellulosimicrobium funkei SNG1 in the in vitro biofilm degradation

Boddapati

Gummadi

2023

Biotech and App Biochem

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Mutanase (α‐1,3‐glucanase) is an inducible extracellular enzyme with potential medical applications in dentistry. A novel Cellulosimicrobium funkei strain SNG1 producing mutanase enzyme using α‐1,3‐glucans was isolated, and the enzyme was optimized for increased productivity using the one‐factor‐at‐a‐time approach. Maximum growth and enzyme‐specific activity (2.12 ± 0.4 U/mg) were attained in a production medium with pH 7.0 and 1% α‐1,3‐glucans as carbon source, incubated at 37°C for 30 h. The result showed a five‐fold increase in activity compared to unoptimized conditions (0.40 U/mg). The enzyme was purified by gel‐filtration chromatography, and recovered with a yield of 29.03% and a specific activity increase of 10.9‐fold. The molecular mass of the monomeric enzyme is 137 kDa. The pH and temperature optima are 6.0 and 45°C with Km of 1.28 ± 0.11 mg for α‐1,3‐glucans. The enzyme activity was stimulated by adding Co2+, Ca2+, Cu2+, and was entirely inhibited by Hg2+. On 2‐h incubation, the purified enzyme effectively degraded in vitro film with an 82.68% degradation rate and a saccharification yield of 30%.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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A comprehensive review on mutan (a mixed linkage of α-1-3 and α-1-6 glucans) from bacterial sources

Cited by 5 publications

References 127 publications

Gellan Gum as a Unique Microbial Polysaccharide: Its Characteristics, Synthesis, and Current Application Trends

Gellan Gum as a Unique Microbial Polysaccharide: Its Characteristics, Synthesis, and Current Application Trends

Production and application of purified mutanase from novel Cellulosimicrobium funkei SNG1 in the in vitro biofilm degradation

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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