A comprehensive review on tissue culture studies and secondary metabolite production in <i>Bacopa monnieri</i> L. Pennell: a nootropic plant

Sudheer, Wudali Narasimha; Thiruvengadam, Muthu; Nagella, Praveen

doi:10.1080/07388551.2022.2085544

Cited by 17 publications

(13 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPLC analysis confirmed the presence of four triterpenoid saponin glycosides (bacoside A3, bacopaside X, bacopaside II, and bacosaponin C) from the in vitro grown biomass treated with various PGRs, precursor, and elicitors as well as acclimatized plantlets. On the other hand, several researchers identified and quantified bacoside saponins from B. monnieri and also reported potent biological activities like anti-inflammatory, antioxidant, anti-hepatotoxic, and analgesic and superoxide inhibition ( Bhandari et al., 2006 ; Sudheer et al., 2022 ). It is a classified and well-known drug used in the improvement of memory or mental power ( Ganzera et al., 2004 ; Deepak et al., 2005 ; Bhandari et al., 2006 ; Naik et al., 2012 ; Saini et al., 2012 ; Bansal et al., 2014 ; Christopher et al., 2017 ; Bhandari et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…In our studies, also the combination of auxins (MS + 0.5 IAA mg/l + 0.5 IBA mg/l + 1.0 mg/l NAA) promoted the root initiation, elongation, and number of roots per shoot. According to earlier reports, rooting of the in vitro produced shoots of B. monnieri was higher when auxin-rich MS medium was used (Banerjee and Shrivastava, 2008;Sudheer et al, 2022). Selection of PGRs plays a crucial role in in vitro propagation of the plant.…”

mentioning

confidence: 98%

See 1 more Smart Citation

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

et al. 2023

View full text Add to dashboard Cite

Bacopa floribunda (Family: Plantaginaceae) is used in folklore medicines for the management of cognitive dysfunction. It has nootropic, antiaging, anti-inflammatory, anti-cholinesterase, and antioxidant properties. We developed an efficient and reproducible protocol for in vitro propagation of B. floribunda using the nodal explants. We assessed the effects of Murashige and Skoog (MS) medium fortified with various plant growth regulatory substances (PGRs), a precursor, and elicitors and their optimal combinations on regeneration and production of total saponins, triterpenoid saponin glycosides (bacoside A3, bacopaside X, bacopaside II, and bacosaponin C), and stigmasterol content in in vitro grown biomass of B. floribunda. The growth of the shoots and roots was stimulated by MS + 2.0 mg/l BAP + 2.0 mg/l KIN and MS + 0.5 mg/l IAA + 0.5 mg/l IBA + 1.0 mg/l NAA, respectively. After 10 weeks of acclimatization, plantlets of B. floribunda had a survival rate of 95%. The highest total saponin content (35.95 ± 0.022 mg DE/g DW) was noted in the treatment of MS + 2.0 mg/l BAP + 1.5 μM SQ. Similarly, total triterpenoid saponin glycosides and stigmasterol were found maximum in biomass derived from MS + 2.0 mg/l BAP + 1.5 μM SQ and MS + 2.0 mg/l BAP, respectively. At the same treatments, bacoside A3 (1.01 ± 0.195 mg/g DW), bacopaside II (43.62 ± 0.657 mg/g DW), bacopaside X (1.23 ± 0.570 mg/g DW), bacosaponin C (0.19 ± 0.195 mg/g DW), and stigmasterol (7.69 ± 0.102 mg/g DW) were reported. The present findings will help to highlight B. floribunda as a potent memory-enhancing herb, and in future also, it could be a potential substitute to B. monnieri. The current work is the first to describe the micropropagation and the elicited production of bioactive metabolites from the in vitro grown biomass of B. floribunda. In addition, further research is needed on production of bioactives, their pharmacological effects, and the elicited production using callus, cell suspension, and hairy root cultures.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Plant micropropagation is one of the optimum biotechnological approaches to overcome many global problems such as desertification, salinization, climate change, and the global water crisis. Applied plant biotechnology via micropropagation is considered a part of the sustainable solution to meet the goal of 'zero hunger' by increasing food security [149,150].…”

Section: Discussionmentioning

confidence: 99%

An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

View full text Add to dashboard Cite

The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

show abstract

“…It is also recognized for its antioxidant, anti-inflammatory, and antihepatotoxic properties (Sudheer et al, 2023;Fatima et al, 2022). It holds significant importance as a medicinal plant due to the presence of bacosides (Jeena et al, 2017;Murthy, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…This plant possesses a wide range of pharmacological properties and is employed as a neuro‐tonic and medication for mental health, depression, and cognitive function. It is also recognized for its antioxidant, anti‐inflammatory, and anti‐hepatotoxic properties (Sudheer et al, 2023; Fatima et al, 2022). It holds significant importance as a medicinal plant due to the presence of bacosides (Jeena et al, 2017; Murthy, 2022).…”

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate inducible UGT79A18 is a novel glycosyltransferase involved in the bacoside biosynthetic pathway in Bacopa monnieri

Kumar,

Singh,

Lahane

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Bacosides are dammarane‐type triterpenoidal saponins in Bacopa monnieri and have various pharmacological applications. All the bacosides are diversified from two isomers, i.e., jujubogenin and pseudojujubogenin. The biosynthetic pathway of bacoside is not well elucidated. In the present study, we characterized a UDP‐glycosyltransferase, UGT79A18, involved in the glycosylation of pseudojujubogenin. UGT79A18 shows higher expression in response to 5 h of wounding, and 3 h of MeJA treatment. The recombinant UGT79A18 shows in vitro activity against a wide range of flavonoids and triterpenes and has a substrate preference for protopanaxadiol, a dammarane‐type triterpene. Secondary metabolite analysis of overexpression and knockdown lines of UGT79A18 in B. monnieri identify bacopasaponin D, bacopaside II, bacopaside N2 and pseudojujubogenin glucosyl rhamnoside as the major bacosides that were differentially accumulated. In the overexpression lines of UGT79A18, we found 1.7‐fold enhanced bacopaside II, 8‐fold enhanced bacopasaponin D, 3‐fold enhanced pseudojujubogenin glucosyl rhamnoside, and 1.6‐fold enhanced bacopaside N2 content in comparison with vector control plant, whereas in the knockdown lines of UGT79A18, we found 1.4‐fold reduction in bacopaside II content, 3‐fold reduction in the bacopasaponin D content, 2‐fold reduction in the pseudojujubogenin glucosyl rhamnoside content, and 1.5‐fold reduction in bacopaside N2 content in comparison with vector control. These results suggest that UGT79A18 is a significant UDP glycosyltransferase involved in glycosylating pseudojujubogenin and enhancing the pseudojujubogenin‐derived bacosides.

show abstract

A comprehensive review on tissue culture studies and secondary metabolite production in Bacopa monnieri L. Pennell: a nootropic plant

Cited by 17 publications

References 61 publications

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb

An Academic and Technical Overview on Plant Micropropagation Challenges

Methyl jasmonate inducible UGT79A18 is a novel glycosyltransferase involved in the bacoside biosynthetic pathway in Bacopa monnieri

Contact Info

Product

Resources

About