A Comprehensive Study on Capillary Surface Modifications for Electrophoretic Separations of Liposomes
Alice Šimonová,
Renata Píplová,
Martin Balouch
et al.
Abstract:Electroosmotic flow significantly impacts the resolution of separations in capillary electrophoresis and its modification is often necessary. Coating of the inner capillary surface either dynamically or permanently offers a way to alter the electroosmotic flow, potentially reducing the adsorption of analytes, in our case liposomes, to the capillary wall. At first, we measured fluorescently labeled liposomes in an uncoated capillary by capillary electrophoresis with laser-induced fluorescence detection. We used… Show more
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