A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response

Sharma, Eshan; Borah, Pratikshya; Kaur, Amarjot; Bhatnagar, Akanksha; Mohapatra, Trilochan; Kapoor, Sanjay; Khurana, Jitendra P.

doi:10.1016/j.ygeno.2021.03.007

Cited by 31 publications

(20 citation statements)

References 76 publications

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“…The binding of ABA to PYL proteins inhibits protein phosphatase type 2Cs (PP2Cs) activity, thereby activating SNF1-related kinases ( SnRK2s ) to phosphorylate and activate ABFs [ 65 ]. A study on the heat-tolerant rice cultivar “Annapurna” showed that PP2Cs and ABFs were upregulated at 37 °C temperature stress [ 22 ]. In agreement, all the PP2Cs and most ABFs were upregulated by heat stress in C. tangshen , indicating the critical role of the ABA signaling pathway in C. tangshen thermotolerance.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Jiang

Wassie

Zhou

et al. 2023

Life

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Codonopsis tangshen Oliv (C. tangshen) is a valuable traditional Chinese medicinal herb with tremendous health benefits. However, the growth and development of C. tangshen are seriously affected by high temperatures. Therefore, understanding the molecular responses of C. tangshen to high-temperature stress is imperative to improve its thermotolerance. Here, RNA-Seq analysis was performed to investigate the genome-wide transcriptional changes in C. tangshen in response to short-term heat stress. Heat stress significantly damages membrane stability and chlorophyll biosynthesis in C. tangshen, as evidenced by pronounced malonaldehyde (MDA), electrolyte leakage (EL), and reduced chlorophyll content. Transcriptome analysis showed that 2691 differentially expressed genes (DEGs) were identified, including 1809 upregulated and 882 downregulated. Functional annotations revealed that the DEGs were mainly related to heat shock proteins (HSPs), ROS-scavenging enzymes, calcium-dependent protein kinases (CDPK), HSP-HSP network, hormone signaling transduction pathway, and transcription factors such as bHLHs, bZIPs, MYBs, WRKYs, and NACs. These heat-responsive candidate genes and TFs could significantly regulate heat stress tolerance in C. tangshen. Overall, this study could provide new insights for understanding the underlying molecular mechanisms of thermotolerance in C. tangshen.

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Section: Discussionmentioning

confidence: 99%

“…Plant hormone signaling transduction could also play a remarkable role in heat stress response. Several ABA metabolism and auxin-associated genes have been identified in A. thaliana and rice exposed to heat stress, respectively [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Jiang

Wassie

Zhou

et al. 2023

Life

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“…These primary signaling events are the initial elements of stress tolerance at the cell level and provide vital clues to acclimation mechanisms in stress-tolerant genotypes. For example, abscisic acid (ABA) plays a central role in response to drought [7], salinity [8] and extreme temperatures [9] and triggers many acclimation responses in plants [10]. However, downstream secondary molecular and metabolic events are vastly diverse and can be peculiar to one or more stress factors [11].…”

Section: Introductionmentioning

confidence: 99%

Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars

Habibpourmehraban

et al. 2022

IJMS

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Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting in yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure to combined drought, salt and extreme temperature treatments, following a 2 day salinity pre-treatment in two rice genotypes—Nipponbare (a paddy rice) and IAC1131 (an upland landrace). Stomata closed after two days of combined stresses, causing intercellular CO2 concentrations and assimilation rates to diminish rapidly. Abscisic acid (ABA) levels increased at least five-fold but did not differ significantly between the genotypes. Tandem Mass Tag isotopic labelling quantitative proteomics revealed 6215 reproducibly identified proteins in mature leaves across the two genotypes and three time points (0, 2 and 4 days of stress). Of these, 987 were differentially expressed due to stress (cf. control plants), including 41 proteins that changed significantly in abundance in all stressed plants. Heat shock proteins, late embryogenesis abundant proteins and photosynthesis-related proteins were consistently responsive to stress in both Nipponbare and IAC1131. Remarkably, even after 2 days of stress there were almost six times fewer proteins differentially expressed in IAC1131 than Nipponbare. This contrast in the translational response to multiple stresses is consistent with the known tolerance of IAC1131 to dryland conditions.

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“…Auxin (IAA) is the second hormone after ABA that positively regulates seed dormancy [ 18 ]. In response to heat stress induction, the expression levels of the growth hormone synthesis genes OsIAA13 and OsIAA20 were increased, and the growth hormone response gene SAUR was also substantially induced to be expressed [ 19 ]. The elongation and growth of Arabidopsis hypocotyl under heat stress is related to the increase in the auxin content [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Betaine Enhances the Protrusion Vigor of Rice Seeds under Heat Stress by Regulating Plant Hormone Signal Transduction and Its Interaction Network

Qian

Liu

et al. 2022

Antioxidants

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Rice is an important food crop. Rice seedlings are mainly composed of root, coleoptile, mesocotyl and euphylla. The elongation of coleoptile and mesocotyl promotes the emergence of rice seedlings. Therefore, analyzing the mechanism of coleoptile and mesocotyl elongation is important for the cultivation of rice varieties. Due to global warming, heat stress is threatening rice yields. Betaine plays an important role in plant resistance to heat stress; however, we lack research on its regulation mechanism of rice seed germination under heat stress. Therefore, we explored the effects of soaking seeds with betaine at different concentrations on rice seed germination under heat stress. According to the results, soaking seeds with 10 mM of betaine could effectively improve the seeds’ germination potential and rate under heat stress to promote the germination of rice seeds. To clarify the mitigation mechanism of betaine in heat stress, we measured the antioxidant enzyme activity, malondialdehyde content, soluble protein content and endogenous hormone content of seed protrusion under heat stress. We constructed the cDNA library for transcriptome sequencing. According to the results, 10 mM of betaine improved the activities of the superoxide dismutase, peroxidase and catalase of seed protrusion under heat stress to reduce the malondialdehyde content and increase the soluble protein content to alleviate the effect of heat stress on rice seed germination. The detection of the endogenous hormone content showed that soaking seeds with 10 mM of betaine increased the content of gibberellin and decreased the contents of auxin and abscisic acid of seed protrusion under heat stress. According to the transcriptome analysis, betaine can induce the expressions of key genes in the biosynthesis and metabolism of auxin, abscisic acid and gibberellins in the seed coleoptile and mesocotyl elongation stage, regulate the signal transduction of three hormones and promote the germination of rice seeds under heat stress. This study revealed, for the first time, the physiological and molecular regulation mechanism of betaine promotion of seed germination under heat stress.

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A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response

Cited by 31 publications

References 76 publications

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars

Exogenous Betaine Enhances the Protrusion Vigor of Rice Seeds under Heat Stress by Regulating Plant Hormone Signal Transduction and Its Interaction Network

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