2019
DOI: 10.1039/c9ja00164f
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A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Abstract: This concise tutorial review provides a description of the current state of the art in the application of time-of flight based secondary ion mass spectrometry (TOF-SIMS) in the field of molecular and cellular imaging.

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Cited by 67 publications
(47 citation statements)
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“…large Ar clusters. 270 High ion doses allow depth proling. 270 NanoSIMS studies of 300 nm-thick sections of resinembedded colon cancer cells treated with 15 N-labelled cisplatin (10-150 mM) suggest partial dissociation of Pt-N bonds in at least the nucleoli, based on the Pt/N stoichiometry, and co-localisation of Pt with S and P (detection of 194 Pt, 34 S and 15 N signals), consistent with the affinity of Pt II for S-rich proteins and DNA.…”
Section: Imaging Mass Spectrometrymentioning
confidence: 99%
See 1 more Smart Citation
“…large Ar clusters. 270 High ion doses allow depth proling. 270 NanoSIMS studies of 300 nm-thick sections of resinembedded colon cancer cells treated with 15 N-labelled cisplatin (10-150 mM) suggest partial dissociation of Pt-N bonds in at least the nucleoli, based on the Pt/N stoichiometry, and co-localisation of Pt with S and P (detection of 194 Pt, 34 S and 15 N signals), consistent with the affinity of Pt II for S-rich proteins and DNA.…”
Section: Imaging Mass Spectrometrymentioning
confidence: 99%
“…270 High ion doses allow depth proling. 270 NanoSIMS studies of 300 nm-thick sections of resinembedded colon cancer cells treated with 15 N-labelled cisplatin (10-150 mM) suggest partial dissociation of Pt-N bonds in at least the nucleoli, based on the Pt/N stoichiometry, and co-localisation of Pt with S and P (detection of 194 Pt, 34 S and 15 N signals), consistent with the affinity of Pt II for S-rich proteins and DNA. 271 Similarly, correlative nanoSIMS and electron microscopy imaging of Au III mesoporphyrin IX dimethyl ester (Au2) in A2780 cells suggests that this anticancer complex attacks sulfurrich proteins in mitochondria ( 197 Au in regions with high 32 S/ 12 C 14 N ratios).…”
Section: Imaging Mass Spectrometrymentioning
confidence: 99%
“…In both LESA and 3D OrbiSIMS, ions are generated in a complementary manner. Whilst nanoESI predominantly generates adduct ions of the parent molecule, SIMS spectra can contain numerous peaks which can be either intact molecules or fragments thereof (35). This makes the automated assignment of SIMS secondary ions to metabolite identities a challenge.…”
Section: Resultsmentioning
confidence: 99%
“…The resulting composition, corresponding to different depths of the sputtering process and planar position of the rastering primary beam, is used to reconstruct the 3D cross section of the specimen, complementing the results from STEM and EDX spectroscopy. However, appropriate calibration standards are required for quantitative depth-profiling [ 68 ]. ToF-SIMS has been used to understand the depth distribution of oxides after SIS treatment, usually adopted in homopolymer layers such as PMMA [ 56 , 63 ] and PS [ 56 ], PET film and fibers [ 54 ], but also in block copolymers layers such as PS- b -P2VP both as micellar films [ 58 ] and self-assembled nanodomains infiltrated with [ 43 ].…”
Section: Characterization Techniquesmentioning
confidence: 99%