A Confocal Laser Scanning Microscope Investigation of the Epiphany Obturation System

Gharib, Saman R.; Tordik, Patricia A.; Imamura, Glen M.; Baginski, Thomas A.; Goodell, Gary G.

doi:10.1016/j.joen.2007.03.011

Cited by 120 publications

(143 citation statements)

References 25 publications

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“…In this study, more gaps were observed at the apical level of all sealer types than at the coronal level. This observation was consistent with those of other studies 30,31) . The discrepancy between the apical and coronal levels might be accounted for by the lower density and diameter of dentinal tubules found at the apical level, resulting in lower sealer penetration 32) .…”

Section: Discussionsupporting

confidence: 94%

Interfacial adaptation and thickness of bioceramic-based root canal sealers

Al‐Haddad

Kasim

Aziz

2015

Dental Materials Journal

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This study evaluated and compared the sealer thickness and interfacial adaptation of bioceramic sealers (Sankin Apatite III, MTA Fillapex ® , EndoSequence ® BC) to root dentin against AH Plus ® sealer. Sixty extracted single-root premolars were prepared and equally divided into four groups. Sealers were labeled with 0.1% Rhodamine B fluorescent dye. Roots were dissected along the transverse plane at 1 mm (apical), 3 mm (middle), and 6 mm (coronal) levels. Sealer-to-whole canal area ratio was evaluated. Percentage of gap-containing region to canal circumference was calculated using a confocal laser microscope. Sealer thickness was significantly higher at apical and middle levels than at coronal level. EndoSequence BC had the significantly highest thickness compared with MTA Fillapex and AH Plus. The coronal level had significantly less interfacial gaps compared with apical and middle levels. Bioceramic sealers showed more gaps compared with AH Plus, with no significant differences among them.

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Section: Discussionsupporting

confidence: 94%

Interfacial adaptation and thickness of bioceramic-based root canal sealers

Al‐Haddad

Kasim

Aziz

2015

Dental Materials Journal

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“…This improves upon the traditional SEM analysis since it is possible to determine the total amount of sealer penetration into the dentin tubules (5,(20)(21)(22)(23) while avoiding the drying process and alcohol dehydration required when using the conventional high-vacuum SEM, which might result in loss of the root canal sealer (20). CLSM provides observations under environmental conditions with no special specimen processing, resulting in a lower production of technique artifacts (24)(25)(26).…”

Section: Discussionmentioning

confidence: 99%

“…The roots were filled with gutta-percha and labeled with AH Plus. After storage, these samples were sectioned and prepared for CLSM analysis (5,14,15). The analysis of these specimens showed very little or no sealer penetration in the three root thirds (Figure 1a, b, c).…”

Section: Confocal Laser Scanning Microscope Controlmentioning

confidence: 99%

Epoxy Resin-Based Root Canal Sealer Penetration into Dentin Tubules Does not Improve Root Filling Dislodgement Resistance

De‐Deus¹,

BrandÓo²,

Souza³

et al. 2017

Eur Endod J

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Objective: This study sought to evaluate the effect of the penetration of an epoxy resin-based root canal sealer into dentin tubules on the force required to dislodge the root canal filling. Methods: Sixty extracted human central incisors with single canals were decoronated, instrumented, and filled with gutta-percha and AH Plus sealer labeled with 0.1% rhodamine B dye. The roots were further sectioned horizontally at 3, 6, and 8 mm from the apex. The coronal surfaces of the resulting 180 slices were evaluated using confocal laser scanning microscopy to measure the amount of sealer that penetrated into the dentin tubules. To quantify the force required to dislocate the root filling material, the root fillings of the slices were subjected to a dislodgement resistance test (push-out). Spearman's rho correlation test was further used to test the correlation between the push-out bond strength and sealer penetration into the dentin tubules (P<0.05). Results: No significant correlation was observed between sealer penetration into the dentin tubules and the force required to dislodge the root canal filling (P=0.626). Conclusion:Following the results of this study, the penetrating ability of the AH Plus sealer into dentin tubules has no correlation with the force required to dislodge the root canal filling.

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“…2). Buccal, lingual, mesial and distal points were established in each image based on the proposed methodologies (13)(14)(15) and linear gauge (in µm) of each measurement was made by adding and calculating the mean value between them (Fig. 3).…”

Section: Data Tabulation and Statistical Analysismentioning

confidence: 99%

Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

et al. 2015

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The aim of this study was to evaluate the filling effectiveness and dentinal penetration of the sealers AH Plus, Pulp Canal Sealer EWT, Sealapex and MTA Fillapex applied according to the vertical condensation technique using thermoplastic gutta-percha. Forty singlerooted teeth were selected. After chemical-mechanical preparation and root-canal filling, sections of the root (2, 4 and 6 mm from the apex) were obtained and analyzed by stereo microscopy and confocal laser scanning microscopy. Data were tabulated and statistically analyzed. With regard to the assessment of void spaces in the filling material at 2 mm from the apex, the sealers showed similar results, but at 4 and 6 mm from the apex, MTA Fillapex had inferior performance compared to AH Plus (at 4 mm), Pulp Canal Sealer EWT (at 4 and 6 mm) and Sealapex (at 6 mm) (p<0.05). With regard to the penetration into dentinal tubules at 2 mm from the apex, the sealers also showed similar results, but at 4 and 6 mm Pulp Canal Sealer EWT had an inferior performance compared to MTA Fillapex and AH Plus, respectively (p<0.05). All four sealers were found to be similar regarding adaptation of the filling material to the root canal walls, except the MTA Fillapex, which showed failures at 4 and 6 mm from the root apex. With regard to the ability to penetrate into the dentinal tubules, the sealers were found to be equivalent, except for the Pulp Canal Sealer EWT as it had poorer results at 4 and 6 mm compared to MTA Fillapex and AH Plus, respectively.

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A Confocal Laser Scanning Microscope Investigation of the Epiphany Obturation System

Cited by 120 publications

References 25 publications

Interfacial adaptation and thickness of bioceramic-based root canal sealers

Interfacial adaptation and thickness of bioceramic-based root canal sealers

Epoxy Resin-Based Root Canal Sealer Penetration into Dentin Tubules Does not Improve Root Filling Dislodgement Resistance

Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

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