A conserved nucleotide sequence at the sites of developmentally regulated chromosomal breakage in tetrahymena

Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes

“…To ascertain the existence of Cbs, we surveyed whole sequence of Ma-(0.62). Any consensus Cbs, which was identified in Euplotes and Tetrahymena (Yao et al, 1987;Klobutcher et al, 1998), was not detected as well as previous studies in Paramecium (Baroin et al, 1987;Coyne et al, 1996;Le Mouël et al, 2003).…”

“…Cbs has been identified so far in the vicinity of chromosome fragmentation sites. Classic Cbs have the sequence TAAACCAAC-CTCTTT in Tetrahymena (Yao et al, 1987), and a 10 bp conserved sequence element (5'-HATTGAAaHH-3', H = A, C or T) in Euplotes near chromosome fragmentation sites (Klobutcher et al, 1998). These cis-acting sequences are necessary and sufficient for site-specific chromosome fragmentation.…”

Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional SitesSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY653004, AY653005, AY653006, AY653007, AY653008, AY653009, AY653010, AY653011, AY653012, AY653013, AY653014, AY653015, AY653016, AY653017, AY653018, AY653019, AY653020, AY653021, AY653022, AY653023, AY653024, AY653025, AY653026, AY653027, AY653028, AY653029.

“…Functional Cbs elements with variant sequence: Two observations suggested that the Cbs libraries were unlikely to be complete: 3L-1, and 3L-2) were independent reisolations of difference; the allele assorted to purity is indicated under each junctions originally described by Yao et al (1987).…”

“…The results of one such If the Cbs is indeed functional, then chromosome breakage should have led to telomere addition at predictable test are illustrated in Figure 2. Using this approach, Cbs ; Tt-prefixed Cbs, originally characterized by Yao et al (1987); those reisolated from the Cbs library are in parentheses.…”

“…(1987) Yao et al (1987) were assigned the lowest serial numbers for their correeither side are deleted (Fan and Yao 1996) total volume of 9 l in a 1.5-ml microfuge tube. The micronuclear DNA was denatured with 1 l of 1 n NaOH (0.1 n final) for 5 min at room temperature.…”