A convenient diagnostic polymerase chain reaction method for identifying codling moth Cydia pomonella (Lepidoptera: Tortricidae) among tortricid pests in cherries imported from western North America

Amano, Takayuki; Higo, Yuichi

doi:10.1007/s13355-015-0360-9

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…Until now, the diagnostic methods to identify C. pomonella mainly include the morphological characteristics and molecular methods such as the mitochondrial COI gene 13,14,15 , real-time quantitative PCR 16,17 , specie-speci c COI 16,18 , and PCR-RFLP 19 . However, these molecular methods need a complicated DNA extraction process and a laboratory setting, which is not suitable for on-site diagnostics 20 .…”

Section: Introductionmentioning

confidence: 99%

Development of LAMP and droplet digital PCR methods for differentiation between Cydia pomonella (L.) and Grapholita molesta (Busck)

yang,

Zhang,

Zhang

et al. 2024

Preprint

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The codling moth, Cydia pomonella (L.), is an economically important key fruit pest worldwide. In China, C. pomonella was first discovered in 1953 and has since been introduced into at least eight provinces. The monitoring of C. pomonella using sex pheromones is essential for controlling this destructive pest and preventing its spread from infested areas. However, the sex pheromone of C. pomonella also has strong attractive effects on Grapholita molesta (Busck), which results in the mixture of the two pest insects. Furthermore, capturing individuals, especially during the early phase of spread, is challenging due to the limited number of introductions. Thus, it is crucial to provide an accurate and rapid diagnostic method to differentiate them. To develop such a method for distinguishing between C. pomonella and G. molesta, we initially selected a set of C. pomonella specific-LAMP primers from seven designed sets of candidate primers and its sensitivity was evaluated using DNA. Finally, the effectiveness of the method was proven using insect tissue and a temperature-controlled, insulated cup. Additionally, the optimal reaction temperature, specificity, and sensitivity of the C. pomonella ddPCR-primer were determined. The development of the C. pomonella LAMP and ddPCR methods provide tools for the monitoring of C. pomonella in China.

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Section: Introductionmentioning

confidence: 99%

Development of LAMP and droplet digital PCR methods for differentiation between Cydia pomonella (L.) and Grapholita molesta (Busck)

yang,

Zhang,

Zhang

et al. 2024

Preprint

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“…The European leaf roller, Archips rosana (Linnaeus, 1758), is a defoliator of hardwood trees and shrubs that is distributed throughout Europe, North Africa, North America, Middle East, Iran, Iraq, Turkey, Azerbaijan and Kazakhstan (Ferriere, 1941;Kapidani & Duraj, 1991;Ulusoy, Vatansever, & Uygun, 1999;Güncan, Yoldaș, & Koçlu, 2013;Amano & Higo, 2015). The larvae of A. rosana roll and tie leaves together for shelter and feeding.…”

Section: Introductionmentioning

confidence: 99%

New Records of the Craneflies (Diptera: Limoniidae, Tipulidae) from the Western Balkans

Bilalli¹,

IBRAHIMI²,

MUSLIU³

et al. 2021

jers

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This is the first report for Iran of Dolichogenidea laevigata (Ratzeburg, 1848) (Hymenoptera: Braconidae: Microgastrinae) parasitizing larvae of the European leaf roller Archips rosana (Linnaeus, 1758) (Lepidoptera: Tortricidae). The parasitized larvae of A. rosana were obtained from different host plants. The occurrence of D. laevigata and its diagnosis are presented. In addition, a full DNA barcode (the mitochondrial cytochrome oxidase subunit I, (COI) barcoding region) of the species was obtained for the first time.

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Identification of three internal feeders from Korla fragrant pears by quantitative real-time polymerase chain reaction

Wang

Feng

et al. 2017

Mitochondrial DNA Part A

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Cydia pomonella, Euzophera pyriella Yang, and Grapholitha molesta are destructive pest species of Korla fragrant pears in Xinjiang. They are also quarantine pests of concern in a number of countries. Identification of these small pest larvae by morphological characters is difficult, and misidentifications will influence appropriate quarantine decisions. Here, a 520 bp fragment of mitochondrial cytochrome oxidase subunit I (COI) was first amplified and sequenced from each species, and a diagnostic region was observed. Subsequently, the species-specific primer and probe sets of quantitative real-time PCR (qPCR) were designed, which amplified a 114-116 bp fragment of COI genes. This method was validated by amplification DNA extracted from single, multiple, and mixed pest samples. Results indicated that this method allows rapid discrimination and reliable identification of larvae, pupae, and adult specimens of all three species, which could help the international export trading of Korla fragrant pears and related products.

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A convenient diagnostic polymerase chain reaction method for identifying codling moth Cydia pomonella (Lepidoptera: Tortricidae) among tortricid pests in cherries imported from western North America

Cited by 5 publications

References 10 publications

Development of LAMP and droplet digital PCR methods for differentiation between Cydia pomonella (L.) and Grapholita molesta (Busck)

Development of LAMP and droplet digital PCR methods for differentiation between Cydia pomonella (L.) and Grapholita molesta (Busck)

New Records of the Craneflies (Diptera: Limoniidae, Tipulidae) from the Western Balkans

Identification of three internal feeders from Korla fragrant pears by quantitative real-time polymerase chain reaction

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