A convenient sampling and noninvasive dried spot method of uric acid in human saliva: Comparison of serum uric acid value and salivary uric acid in healthy volunteers and hyperuricemia patients

Han, Yu; Shi, Qing; Xu, Chunyan; Di, Lei; Zhao, Lili; Jin, Wenlong; Min, Jun Zhe

doi:10.1016/j.jchromb.2021.122528

Cited by 14 publications

(17 citation statements)

References 33 publications

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“…Fig. 3b shows the current response obtained from measuring 200 µM uric acid after reduction process of GO/PEDOT-PSS film in 0.1 M KCl with different number of cycles (10,15,20,25,40). It can be seen with the increasing number of cycles for the reduction of GO/PEDOT-PSS film, the current response from uric acid oxidation was also improved.…”

Section: The Electrochemical Oxidation Of Uric Acid At the Ergo/pedot:pss Modified Gcementioning

confidence: 89%

“…Previously, various techniques have been employed to determine the uric acid concentration such as gas chromatography-mass spectrometry 8 , high-performance liquid chromatography [9][10] , UV-Vis spectrophotometry 11 , chemiluminescence 12 , fluorescence. 13 However, these methods are reported complex to perform, time consuming, laborious, and requires expensive equipment thus impractical for daily analysis.…”

Section: Introductionmentioning

confidence: 99%

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A facile electrochemical sensor based on a composite of electrochemically reduced graphene oxide and a PEDOT:PSS modified glassy carbon electrode for uric acid detection

et al. 2022

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A glassy carbon electrode modified with electrochemically reduced graphene oxide (ErGO) and poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) was developed for the uric acid determination with dopamine as interference in artificial saliva. The electrochemical performance of the fabricated electrode was studied using both techniques of cyclic voltammetry (CV) and differential pulse voltammetry (DPV), under optimized conditions. Using DPV, the sensor based on ErGO/PEDOT:PSS modified GCE displayed a linear relationship in the concentration ranges of 10-100 µM for uric acid. This uric acid sensor exhibited a high sensitivity with the detection limits of 1.08 µM and the quantitation limit of 3.61 µM. This sensor also showed good reproducibility for uric acid detection in the artificial saliva in 5 consecutive days measurements. This device was successfully used to analyze uric acid in the artificial saliva as well as in human saliva sample using standard addition method.

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Section: The Electrochemical Oxidation Of Uric Acid At the Ergo/pedot:pss Modified Gcementioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A facile electrochemical sensor based on a composite of electrochemically reduced graphene oxide and a PEDOT:PSS modified glassy carbon electrode for uric acid detection

et al. 2022

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“…We found that although there existed individual differences in saliva samples between patients, the UA concentration in saliva and blood was positively correlated (Pearson’ r = 0.6927, adjusted R 2 = 0.4509), and for the same sample, the blood UA concentration was higher than that in saliva, as indicated by the positive intercept (Figure a). The slope of the calibration curve was in the normal range from 0.6 to 0.9, and the intercept was in the normal range from 25 to 45 ppm. ,− Therefore, the results measured by our SμPADs are reliable. Singh et al used the uricase–peroxidase method to measure UA concentrations in saliva .…”

Section: Resultsmentioning

confidence: 63%

Digital Quantification Method for Sensitive Point-of-Care Detection of Salivary Uric Acid Using Smartphone-Assisted μPADs

et al. 2022

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Uric acid (UA) is an important biomarker for many diseases. A sensitive point-of-care (POC) testing platform is designed for the digital quantification of salivary UA based on a colorimetric reaction on an easy-to-build smartphone-assisted microfluidic paper-based analytical device (SμPAD). UA levels are quantified according to the color intensity of Prussian blue on the SμPAD with the aid of a MATLAB code or a smartphone APP. A color correction method is specifically applied to exclude the light effect. Together with the engineering design of SμPADs, the background calibration function with the APP increases the UA sensitivity by 100-fold to reach 0.1 ppm with a linear range of 0.1−200 ppm. The assay time is less than 10 min. SμPADs demonstrate a correlation of 0.97 with a commercial UA kit for the detection of salivary UA in clinical samples. SμPADs provide a sensitive, fast, affordable, and reliable tool for the noninvasive POC quantification of salivary UA for early diagnosis of abnormal UA level-associated health conditions.

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“…Two biological sample types, blood and saliva, both carrying natural large proteins and complex macromolecules, were selected to study the sensor response. In cases where conventional blood sampling is challenging or considered invasive, dried saliva spots and dried blood spots can provide a practical alternative for collecting and studying samples [ 84 , 85 ]. Dried spot tests can be collected remotely and studied later in the laboratory, in cases where a portable Raman spectrometer is not available.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Amitriptyline in Dried Blood and Dried Saliva Samples with Surface-Enhanced Raman Spectroscopy

Boroujerdi

Paul

Abdelkader

2022

Sensors

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There is growing demand for rapid, nondestructive detection of trace-level bioactive molecules including medicines, toxins, biomolecules, and single cells, in a variety of disciplines. In recent years, surface-enhanced Raman scattering has been increasingly applied for such purposes, and this area of research is rapidly growing. Of particular interest is the detection of such compounds in dried saliva spots (DSS) and dried blood spots (DBS), often in medical scenarios, such as therapeutic drug monitoring (TDM) and disease diagnosis. Such samples are usually analyzed using hyphenated chromatography techniques, which are costly and time consuming. Here we present for the first time a surface-enhanced Raman spectroscopy protocol for the detection of the common antidepressant amitriptyline (AMT) on DBS and DSS using a test substrate modified with silver nanoparticles. The validated protocol is rapid and non-destructive, with a detection limit of 95 ppb, and linear range between 100 ppb and 1.75 ppm on the SERS substrate, which covers the therapeutic window of AMT in biological fluids.

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A convenient sampling and noninvasive dried spot method of uric acid in human saliva: Comparison of serum uric acid value and salivary uric acid in healthy volunteers and hyperuricemia patients

Cited by 14 publications

References 33 publications

A facile electrochemical sensor based on a composite of electrochemically reduced graphene oxide and a PEDOT:PSS modified glassy carbon electrode for uric acid detection

A facile electrochemical sensor based on a composite of electrochemically reduced graphene oxide and a PEDOT:PSS modified glassy carbon electrode for uric acid detection

Digital Quantification Method for Sensitive Point-of-Care Detection of Salivary Uric Acid Using Smartphone-Assisted μPADs

Rapid Detection of Amitriptyline in Dried Blood and Dried Saliva Samples with Surface-Enhanced Raman Spectroscopy

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