A correlation of immunohistochemical expression of TP53 and CDKN1A in oral epithelial dysplasia and oral squamous cell carcinoma

Pandya, Jay Ashokkumar; Boaz, Karen; Natarajan, Srikant; Manaktala, Nidhi; Nandita, K. P.; Lewis, Amitha J

doi:10.4103/0973-1482.180683

Cited by 10 publications

(4 citation statements)

References 15 publications

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“…Researchers demonstrated p53’s role in differentiating grades of dysplasia. Pandya et al [ 53 ] showed that the difference in expression was statistically significant between mild and severe dysplasia. The difference in TP53 expression between mild and severe dysplasia was statistically significant, according to Patil et al [ 54 ].…”

Section: Discussion Of Potential Immunohistochemical Biomarkers In Gr...mentioning

confidence: 99%

“…Cell division and proliferation p53 [53][54][55]75,76,117], Ki-67 [56,[68][69][70][71][72][73][74][75][76], CD105 [73,74], p63 [39,42,117], CD31 [39], CD34 [43], cycD1 [42], VEGF [43], YAP, Np63 [59], stathmin [80], CDKN1A [53] Epithelial-mesenchymal transition CK5\6 [96], CK19 [98], β-catenin [103,104], N-cadherin [109], E-cadherin [104][105][106][107], TWIST [111], VIM [107,108], PDPN [115,117], MMP-9 [108] Cell death regulation Bcl-2 [123,124], PDCD4 [128], HSP27 [131], cornulin [134] Cellular metabolism HIF-1a [136], iNOS...…”

Section: Relation Of Biomarkers Examples Of Biomarkers For Grading Of...mentioning

confidence: 99%

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Potential Immunohistochemical Biomarkers for Grading Oral Dysplasia: A Literature Review

Zdrojewski,

Nowak,

Nijakowski

et al. 2024

Biomedicines

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Oral cancer is a prevalent global health issue, with significant morbidity and mortality rates. Despite available preventive measures, it remains one of the most common cancers, emphasising the need for improved diagnostic and prognostic tools. This review focuses on oral potentially malignant disorders (OPMDs), precursors to oral cancer, specifically emphasising oral epithelial dysplasia (OED). The World Health Organisation (WHO) provides a three-tier grading system for OED, and recent updates have expanded the criteria to enhance diagnostic precision. In the prognostic evaluation of OED, histological grading is presently regarded as the gold standard; however, its subjectivity and unreliability in anticipating malignant transformation or recurrence pose notable limitations. The primary objective is to investigate whether specific immunohistochemical biomarkers can enhance OED grading assessment according to the WHO classification. Biomarkers exhibit significant potential for comprehensive cancer risk evaluation, early detection, diagnosis, prognosis, and treatment optimisation. Technological advancements, including sequencing and nanotechnology, have expanded detection capabilities. Some analysed biomarkers are most frequently chosen, such as p53, Ki-67, cadherins/catenins, and other proteins used to differentiate OED grades. However, further research is needed to confirm these findings and discover new potential biomarkers for precise dysplasia grading and minimally invasive assessment of the risk of malignant transformation.

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Section: Discussion Of Potential Immunohistochemical Biomarkers In Gr...mentioning

confidence: 99%

Section: Relation Of Biomarkers Examples Of Biomarkers For Grading Of...mentioning

confidence: 99%

Potential Immunohistochemical Biomarkers for Grading Oral Dysplasia: A Literature Review

Zdrojewski,

Nowak,

Nijakowski

et al. 2024

Biomedicines

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“…SENP7 also showed a strong relationship with the chemotherapy drugs (Fig 5A–5C). The networks of SENP7 as well as other targets, KAT2B and DTX3L, with chemotherapy drugs showed the strong relationship with TP53 protein, a biomarker of oral cancers [(Figs 5 and 6) [37, 38]. The association of target proteins with TP53 and chemotherapy drugs, especially carboplatin and doxorubicin, should be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Salivary proteomics of canine oral tumors using MALDI-TOF mass spectrometry and LC-tandem mass spectrometry

Ploypetch¹,

Roytrakul²,

Jaresitthikunchai³

et al. 2019

PLoS ONE

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Canine oral tumors are relatively common neoplasms in dogs. For disease monitoring and early diagnosis, salivary biomarkers are appropriate because saliva collection is non-invasive and requires no professional skills. In the era of omics, matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF MS) coupled with liquid chromatography-tandem MS (LC-MS/MS) are suitable to identify potential disease-associated peptides and proteins. The present study aimed to use MALDI-TOF MS and LC-MS/MS to search for particular peptide mass fingerprints (PMFs) and conceivable biomarkers in saliva of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP). Pooled saliva samples in each group were used to be representative of population change. Unique PMFs were obtained and specific peptide fragments were sequenced by LC-MS/MS and BLAST-searched with mammalian protein databases. Seven peptide fragments appeared in the tumor groups (EOM, LOM, OSCC and BN) at 1096, 1208, 1322, 1794, 1864, 2354 and 2483 Da, two peptide fragments appeared in the LOM and OSCC groups at 2450 and 3492 Da, and in the CP controls at 2544 and 3026 Da. Also, protein–chemotherapy drug interaction networks were exhibited. Using western blot analysis, the expression of sentrin-specific protease 7 (SENP7), a peptide fragment at 1096 Da, in OSCC was significantly increased, as was the expression of TLR4, a peptide fragment at 3492 Da, in LOM and OSCC, compared with the CP group. The expression of nuclear factor kappa B (NF-κB), a TLR4 partner, was notably increased in OSCC compared with CP, BN and EOM. The expression was also enhanced in LOM compared with EOM. Expressed protein sequences from western blots were verified by LC-MS/MS. Western blots were then performed with individual samples in each group. The results showed the elevated expression of TLR4 in LOM and OSCC, compared with that in CP and BN, the increased expression of NF-κB in LOM and OSCC, compared with CP and in LOM compared with BN, and the enhanced expression of SENP7 in LOM and OSCC, compared with that in CP and BN. In conclusion, discrete clusters of EOM, LOM, OSCC, BN and CP groups and potential protein candidates associated with the diseases were demonstrated by salivary proteomics. Western blot analysis verified SENP7, TLR4 and NF-κB as potential salivary biomarkers of canine oral tumors.

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“…Levels of p53 expression increase in association with histologic grade of OED [ 105 ]. In fact, p53 expression is present in 80% of oral epithelial hyperplasia [ 106 ] and increases significantly from OL to OSCC in patient samples [ 81 ].…”

Section: Nuclear Biomarkersmentioning

confidence: 99%

Molecular Biomarkers of Malignant Transformation in Head and Neck Dysplasia

Ranganath

Feng

Franco

et al. 2022

Cancers

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Head and neck squamous cell carcinoma (HNSCC) and its treatments are associated with substantial morbidity, often resulting in cosmetic deformity and loss of physiologic functions including speech and swallowing. Despite advancements in treatment, 5-year survival rates for mucosal malignancies remain below 70%. Effective prevention of HNSCC demands an understanding of the molecular pathways of carcinogenesis. Specifically, defining features of pre-cancerous dysplastic lesions that indicate a better or worse prognosis is necessary to help identify patients who are likely to develop a carcinoma and allow a more aggressive approach to management. There remains a need for identification of biomarkers that can provide both early prognostic and predictive value in clinical decision-making by serving as both therapeutic targets as well as predictors of therapy response. Here, we comprehensively review the most frequently altered molecular biomarkers of malignant transformation in head and neck dysplasia. These markers are involved in a wide range of cellular processes in head and neck carcinogenesis, including extracellular matrix degradation, cell motility and invasion, cell–cell adhesion, solute transport, immortalization, metabolism, the cell cycle and apoptosis, transcription, and cell signaling.

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A correlation of immunohistochemical expression of TP53 and CDKN1A in oral epithelial dysplasia and oral squamous cell carcinoma

Abstract: The expression of TP53 in OED highlights its role in initial carcinogenesis. Although the role of CDKN1A in the cell cycle has been documented, its relationship to various clinical and pathological variables of OSCC and its different treatment modalities could not be adequately assessed.

Cited by 10 publications

References 15 publications

Potential Immunohistochemical Biomarkers for Grading Oral Dysplasia: A Literature Review

Potential Immunohistochemical Biomarkers for Grading Oral Dysplasia: A Literature Review

Salivary proteomics of canine oral tumors using MALDI-TOF mass spectrometry and LC-tandem mass spectrometry

Molecular Biomarkers of Malignant Transformation in Head and Neck Dysplasia

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