A cost-effective smartphone-based device for rapid <i>C</i>-reaction protein (CRP) detection using magnetoelastic immunosensor

Yuan, Zhongyun; Han, Ming‐Yong; Li, Donghao; Hao, Runfang; Guo, Xinwen; Sang, Shengbo; Zhang, Hongpeng; Ma, Xiaoqian; Hu, Jin; Xing, Zhijin; Zhang, Chun

doi:10.1039/d2lc01065h

Cited by 13 publications

(5 citation statements)

References 60 publications

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“…Increase in the production of E-selectin by endothelial cells stimulated by increased recruitment of activated monocytes by E-selectin Infections are the most common trigger of aGVHD [27,28]. Accordingly, in this study, the level of Creactive protein in aGVHD patients, which is considered an indicator of infection or in ammation [29], was signi cantly higher than non-GVHD (Fig. 3A), which implies that the patients with aGVHD had a higher incidence of infections than those without aGVHD.…”

Section: Resultsmentioning

confidence: 87%

E-selectin combined with soluble CD44 as predictors of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

YANG,

WANG,

CHENG

et al. 2024

Preprint

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Background Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the mainly curable treatment options in children with high-risk malignancies, bone marrow failure diseases and inherited metabolic diseases. Acute graft-versus-host disease (aGVHD) accompanied with series of serious complications are the most severe obstacle of allo-HSCT because the early and accurate diagnostic markers and effective treatment are still lacked. Non-organ-specific injury induced activated endothelial cells and tissue integrity biomarkers may have higher specificity for the occurrence and development of aGVHD. Methods The blood from 52 pediatric patients who underwent allo-HSCT including 16 recipients with aGVHD and 36 recipients without aGVHD were collected to check the level of adhesion molecules. The vitro experiments, transwell experiments, and aGVHD mouse model are used to verify the effects of E-selectin in the occurrence and development of aGVHD. Results We found that E-selectin secreted by endothelial cells was remarkably increased while the level of soluble CD44, a widely distributed tissue structure molecule, was significantly decreased in aGVHD patients. The level of E-selectin was negatively correlated with the soluble CD44 and associated with the severity of the aGVHD. After that, the vitro experiments suggested the elevated E-selectin could recruit immune cells that result in a series of inflammatory response and tissue injury. The aGVHD mouse model revealed that the level of E-selectin in the intestine occurred aGVHD was obviously increased than that without aGVHD. The expression level of CD44 in organs was related to the incidence of organ aGVHD. More importantly, the area under the ROC curve (AUC) of E-selectin and CD44 can reach 0.85 indicating that these two parameters have strong prediction ability of aGVHD. Conclusions E-selectin and CD44 could play an important role in the occurrence and development of aGVHD. E-selectin combined with soluble CD44 could act as efficient biomarkers for the diagnosis of aGVHD.

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Section: Resultsmentioning

confidence: 87%

E-selectin combined with soluble CD44 as predictors of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

YANG,

WANG,

CHENG

et al. 2024

Preprint

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“…52 Lastly, a novel smartphone-based device merges magnetoelastic immunosensing and smartphone technology for self-detection of CRP, with an LOD of 0.2349 ng/mL. 53 The summarized comparisons of the biosensors are outlined in Table 2 below.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The real-time nanoplasmonic immunoturbidimetry assay (NanoPITA) quantifies hypersensitive CRP with a reduced LOD of 0.54 ng/mL . Lastly, a novel smartphone-based device merges magnetoelastic immunosensing and smartphone technology for self-detection of CRP, with an LOD of 0.2349 ng/mL . The summarized comparisons of the biosensors are outlined in Table below.…”

Section: Resultsmentioning

confidence: 99%

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Lin,

Chen,

Huang

et al. 2024

ACS Sens.

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Surface-enhanced Raman scattering (SERS) technology has been widely recognized for its remarkable sensitivity in biochip development. This study presents a novel sandwich immunoassay that synergizes SERS with magnetoplasmonic nanoparticles (MPNs) to improve sensitivity. By taking advantage of the unique magnetism of these nanoparticles, we further enhance the detection sensitivity of SERS biochips through the applied magnetic field. Despite the high sensitivity, practical applications of SERS biochips are often limited by the issues of stability and reproducibility. In this study, we introduced a straightforward statistical method known as "Gaussian binning", which involves initially binning the two-dimensional Raman mapping data and subsequently applying Gaussian fitting. This approach enables a more consistent and reliable interpretation of data by reducing the variability inherent in Raman signal measurements. Based on our method, the biochip, targeting for C-reactive protein (CRP), achieves an impressive detection limit of 5.96 fg/mL, and with the application of a 3700 G magnetic field, it further enhances the detection limit by 5.7 times, reaching 1.05 fg/mL. Furthermore, this highly sensitive and magnetically tunable SERS biochip is easily designed for versatile adaptability, enabling the detection of other proteins. We believe that this innovation holds promise in enhancing the clinical applicability of SERS biochips.

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“…Development of immunosensors for AD biomarkers that may allow the real-time sharing of the results obtained through a smartphone-based or a portable Wi-Fi-based signal-reading device would enhance the potential for various complex applications and home self-diagnosis, while it would enable exploitation/integration of the “new era” technological advances (Internet of things, artificial intelligence, and machine learning) in the field of AD immunosensors [ 34 , 94 , 95 , 96 , 97 ]. To the best of our knowledge, such an immunosensor has been proposed for the diagnosis of celiac disease [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Immunosensors for Autoimmune-Disease-Related Biomarkers: A Literature Review

Karachaliou

Livaniou

2023

Sensors

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Immunosensors are a special class of biosensors that employ specific antibodies for biorecognition of the target analyte. Immunosensors that target disease biomarkers may be exploited as tools for disease diagnosis and/or follow-up, offering several advantages over conventional analytical techniques, such as rapid and easy analysis of patients’ samples at the point-of-care. Autoimmune diseases have been increasingly prevalent worldwide in recent years, while the COVID-19 pandemic has also been associated with autoimmunity. Consequently, demand for tools enabling the early and reliable diagnosis of autoimmune diseases is expected to increase in the near future. To this end, interest in immunosensors targeting autoimmune disease biomarkers, mainly, various autoantibodies and specific pro-inflammatory proteins (e.g., specific cytokines), has been rekindled. This review article presents most of the immunosensors proposed to date as potential tools for the diagnosis of various autoimmune diseases, such as type 1 diabetes, rheumatoid arthritis, and multiple sclerosis. The signal transduction and the immunoassay principles of each immunosensor have been suitably classified and are briefly presented along with certain sensor elements, e.g., special nano-sized materials used in the construction of the immunosensing surface. The main concluding remarks are presented and future perspectives of the field are also briefly discussed.

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A cost-effective smartphone-based device for rapid C-reaction protein (CRP) detection using magnetoelastic immunosensor

Abstract: C-reaction protein (CRP) is a marker of nonspecific immunity for vital signs and wound assessment, and it can be used to diagnose infections in clinical medicine. However, measuring CRP level...

Cited by 13 publications

References 60 publications

E-selectin combined with soluble CD44 as predictors of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

E-selectin combined with soluble CD44 as predictors of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Immunosensors for Autoimmune-Disease-Related Biomarkers: A Literature Review

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