A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors

Abstract

In this article, we report coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties as the first reversible fluorescent probe that can monitor GSH near NMDA receptors.

Monitoring glutathione dynamics in DNA replication (S-phase) using a two-photon reversible ratiometric fluorescent probe

“…Whereas a recently reported coumarin-based two-photon fluorescent probe showed a turn-on signal in the presence of glutathione near N -methyl- d -aspartate (NMDA) receptors. 24…”

“…Indeed, these Michael acceptor-based fluorescent probes can independently provide quantitative information on GSH at any point of time, because of their ratiometric fluorescence change upon the addition/elimination of GSH. 18–24 The GSH-induced change in fluorescence intensity in two or more emission bands of the ratiometric probe resulted in improved fluorescence properties and quantitative information.…”

Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe

“…7,8 Nevertheless, these approaches suffer from complex sample pre-treatment and difficulty in visualization, which make them unsuitable for real-time and in situ GSH measurement in live cells and in vivo. Due to the advantages of excellent spectral characteristics, high spatiotemporal resolution, high sensitivity and selectivity, various small molecular fluorescent probes have been constructed for different biomolecules, [9][10][11][12][13][14][15] including GSH, [16][17][18][19] based on the strong nucleophilicity and reducibility properties of the mercapto unit. 20,21 However, an efficient imaging tool being capable of dynamically and in situ evaluating GSH fluctuation in early and advanced stages of mice HCC models has not been reported yet.…”

A novel water-soluble naphthalimide-based turn-on fluorescent probe for mercury ion detection in living cells and zebrafish

“…17–23 As a necessary component of probe, a large number of fluorophores including 1,8-naphthalimide, coumarin and rhodamine are used to detect small molecules in vivo and in the environment with the continuous improvement of probe performance requirements. 24–29 Among them, 1,8-naphthalimide and its derivatives have been used as fluorophores to construct a large number of fluorescent probes of Hg 2+ owing to their excellent features including desirable photophysical properties and easy modification. 30–35 These probes detect Hg 2+ based on its specific reaction with Hg 2+ .…”