A Country Between: The Upper Ohio Valley and Its Peoples, 1724-1774

Martin, J.R.; McConnell, Michael N.

doi:10.2307/3536984

Cited by 1 publication

(1 citation statement)

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“…Virus agents, and in particular orthopoxviruses (OPXVs), share the common attributes of high morbidity, potential large diffusion, and relatively-easy development as bioweapons for terrorist attacks. Viruses are the most effective agents and were often employed as bioweapons in the last centuries 10 – 12 . They are more lethal than chemical weapons, more difficult to detect than chemical or nuclear weapons, and their production is less expensive, as basic technologies are available in any biological laboratory.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors

Rakhimbekova,

Kudaibergenov,

Seitkamal

et al. 2023

Sci Rep

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In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 103 to 108 plaque-forming units (PFU), with a limit of detection of around 1.7–4.3 × 103 PFU and a log-quadratic pattern, with a response up to 5 × 10−4 RIU (refractive index units). The specificity was assessed against herpes simplex virus, used as a non-specific control, with the best results obtained with anti-L1R monoclonal antibodies, and through the detection of vaccinia virus/herpes simplex-1 combination. The obtained results provide a real-time viral recognition with a label-free sensing platform, having rapid response and ease of manufacturing, and paving the road to the seamless detection of poxviruses affecting different human and animal species using optical fibers.

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Section: Introductionmentioning

confidence: 99%