A CRISPR/Cas9‐based genome‐editing system for yam (<i>Dioscorea</i> spp.)

Syombua, Easter D.; Zhang, Zheng‐Zhi; Tripathi, Jaindra Nath; Ntui, Valentine Otang; Kang, Minjeong; George, Obiero O.; Edward, Nguu K.; Wang, Kan; Yang, Bing; Tripathi, Leena

doi:10.1111/pbi.13515

Cited by 44 publications

(42 citation statements)

References 8 publications

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“…The genetic information from the domesticated species can be translated to the traditional food plants (See next section for the example of translation of genetic information from Solanum lycopersicum and S. pimpinellifolium to Physalis pruinosa.). With the help of databases such as the National Center for Biotechnology Information (NCBI), identification of target genes for their construction of sgRNA by comparing with a homologous sequence is possible [344]. Softwares are used for the construction of plasmid that carries Cas9, gRNA, and reporter gene along with their promoter.…”

Section: Linking Omics and Gene Editing Tools For Rapid Improvement/domestication Of The Traditional Food Cropsmentioning

confidence: 99%

“…For the validation of CRISPR/Cas9 editing the construct pCas9-gRNA-reporter is introduced into nodal-explants after tissue culture using the Agrobacterium-mediated transformation method. After the regeneration of successful transformed plants, phenotypic and genotyping (using RT-PCR) and screening help to check the mutation effect [344]. A generalized work-flow involving various steps in genome editing for improved varieties is presented in Fig.…”

Section: Linking Omics and Gene Editing Tools For Rapid Improvement/domestication Of The Traditional Food Cropsmentioning

confidence: 99%

“…Ji et al [361] have attempted gene editing using CRISPR/Cas9 in Cowpea (Vigna unguiculata) which is also an important traditional food crop because of its symbiotic nitrogen fixation capability. Recently Syombua et al [344] introduced a CRISPR/Cas9-based genome editing system for underutilized yam Dioscorea alata with improved genetic transformation, which can lead to trait improvement in yam. By the establishment of an efficient CRISPR/Cas9 editing protocol, Syombua et al [344] suggested that it is possible to remove undesirable traits of Dioscorea alata such as poor seed set, and non-synchronous flowering.…”

Section: Recent Examples Of Translational Genomics In Successfully Editing Traditional Food Cropsmentioning

confidence: 99%

“…Recently Syombua et al [344] introduced a CRISPR/Cas9-based genome editing system for underutilized yam Dioscorea alata with improved genetic transformation, which can lead to trait improvement in yam. By the establishment of an efficient CRISPR/Cas9 editing protocol, Syombua et al [344] suggested that it is possible to remove undesirable traits of Dioscorea alata such as poor seed set, and non-synchronous flowering. Considering the importance of gene editing technology and its application in successfully editing genes of several crops for improved varieties and a beginning of editing traditional orphan crops, the future studies aiming at extension of this technology to traditional crops will lead to the mainstreaming of many traditional crops.…”

Section: Recent Examples Of Translational Genomics In Successfully Editing Traditional Food Cropsmentioning

confidence: 99%

“…(J) Screening of the regenerated plants for the mutated effect by checking their phenotypes. (K) Genotyping or putative transgenic plants contained Cas9 as confirmed by PCR analysis[333,344,346].…”

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Linking Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified Foods and Sustainable Food Security

Kumar¹,

Anju²,

Kumar³

et al. 2021

Preprint

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The indigenous communities across the globe especially in the rural areas consume locally available plants known as Traditional Food Plants (TFPs) for their nutritional and health-related needs. Recent research shows that many of the traditional food plants are highly nutritious as they contain health beneficial metabolites, vitamins, mineral elements and other nutrients. Excessive reliance on the mainstream staple crops has its own disadvantages. TFPs are nowadays considered important crops of the future and can act as supplementary foods for the burgeoning global population. They can also act as emergency foods in times of pandemics and other situations like COVID-19. The current situation necessitates locally available alternative nutritious TFPs for sustainable food production. To increase the cultivation or improve the traits in TFPs, it is essential to understand the molecular basis of the genes that regulate some important traits such as nutritional components and resilience to biotic and abiotic stresses. The integrated use of modern omics and gene editing technologies provide great opportunities to better understand the genetic and molecular basis of superior nutrient content, climate-resilient traits and adaptation to local agroclimatic zones. Recently, realising the importance and benefits of TFPs, scientists have shown interest in the prospection and sequencing of traditional food plants for their improvements, further cultivation and mainstreaming. Integrated omics such as genomics, transcriptomics, proteomics, metabolomics and ionomics are successfully used in plants and have provided a comprehensive understanding of gene-protein-metabolite networks. Combined use of omics and editing tools has led to successful editing of beneficial traits in few TFPs. This suggests that there is ample scope of integrated use of modern omics and editing tools/techniques for improvement of TFPs and their use for sustainable food production. In this article, we highlight the importance, scope and progress towards improvement of TFPs for valuable traits by integrated use of omics and gene editing techniques.

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Section: Linking Omics and Gene Editing Tools For Rapid Improvement/domestication Of The Traditional Food Cropsmentioning

confidence: 99%

Section: Linking Omics and Gene Editing Tools For Rapid Improvement/domestication Of The Traditional Food Cropsmentioning

confidence: 99%

Section: Recent Examples Of Translational Genomics In Successfully Editing Traditional Food Cropsmentioning

confidence: 99%

Section: Recent Examples Of Translational Genomics In Successfully Editing Traditional Food Cropsmentioning

confidence: 99%

mentioning

confidence: 99%

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Linking Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified Foods and Sustainable Food Security

Kumar¹,

Anju²,

Kumar³

et al. 2021

Preprint

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Potential applications of the CRISPR/Cas technology for genetic improvement of yam (Dioscorea spp.)

Syombua

Tripathi

Obiero

et al. 2021

Food and Energy Security

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Yam (Dioscorea spp.) is an economically important crop grown in the tropical and subtropical regions, producing tuberous roots that serve as a staple food, an income source, and an excellent source of various pharmaceutical precursors. Yam production is constrained by disease and pest infestations and a range of abiotic stresses. Genetic improvement can significantly mitigate these challenges, improve productivity, expand the yam markets, and increase economic gains. However, several intrinsic attributes of the crop have curtailed progress in yam breeding. Advanced genetic engineering such as genome editing by sequence‐specific nucleases has emerged as complementary approaches to conventional breeding techniques. Mainly, the clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas) system for genome editing has provided robust platforms for gene function analysis and crop improvement in the post‐genomic era. Despite its significance, research towards improving the yam species remains under‐represented compared to other staple tuber crops such as cassava and sweet potato. Thus, it is critical to explore avenues for increasing the genetic gains from this under‐exploited crop. The present review focuses on the progress and prospects for applying the CRISPR/Cas technology for yam improvement. The study elaborates on the currently available CRISPR/Cas tool for yam genome engineering and explores the potential applications of this toolkit in mitigating the various challenges encountered in yam production and consumption. Furthermore, we have delved into the challenges associated with this technology and the improvements made to minimize these challenges. The insights presented herein provide a guide for yam improvement to increase genetic gains from this under‐researched and under‐utilized resource.

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