A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

Liu, Haomin; Liu, Yu

doi:10.1016/j.bios.2020.112901

Cited by 49 publications

(32 citation statements)

References 78 publications

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“…Specific substrates, such as ortho-phenyldiamine dihydrochloride, are used to hydrolyze through the top of the enzyme to produce colored results [20]. ELISA has a high sensitivity in detecting protein content, therefore it is the gold standard method for identifying or quantifying proteins [21].…”

Section: Advantages Of Microfluidic Poc Devices In Medical Laboratorymentioning

confidence: 99%

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Yang

Zhang

et al. 2022

Sensors

126

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The early diagnosis of infectious diseases is critical because it can greatly increase recovery rates and prevent the spread of diseases such as COVID-19; however, in many areas with insufficient medical facilities, the timely detection of diseases is challenging. Conventional medical testing methods require specialized laboratory equipment and well-trained operators, limiting the applicability of these tests. Microfluidic point-of-care (POC) equipment can rapidly detect diseases at low cost. This technology could be used to detect diseases in underdeveloped areas to reduce the effects of disease and improve quality of life in these areas. This review details microfluidic POC equipment and its applications. First, the concept of microfluidic POC devices is discussed. We then describe applications of microfluidic POC devices for infectious diseases, cardiovascular diseases, tumors (cancer), and chronic diseases, and discuss the future incorporation of microfluidic POC devices into applications such as wearable devices and telemedicine. Finally, the review concludes by analyzing the present state of the microfluidic field, and suggestions are made. This review is intended to call attention to the status of disease treatment in underdeveloped areas and to encourage the researchers of microfluidics to develop standards for these devices.

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Section: Advantages Of Microfluidic Poc Devices In Medical Laboratorymentioning

confidence: 99%

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Yang

Zhang

et al. 2022

Sensors

126

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“…Most diseases originate in minute amounts of tissue or even in a tiny number of cells, [1][2][3] and slight fluctuations of protein biomarkers may well reflect the physiological change at the early stages of diseases. Therefore, sensitive quantification of circulating protein biomarkers is of great significance for early disease diagnosis, postoperative monitoring and precision medicine.…”

Section: Introductionmentioning

confidence: 99%

All on size-coded single bead set: a modular enrich-amplify-amplify strategy for attomolar level multi-immunoassay

et al. 2022

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“…Biomarkers at the attomolar and femtomolar levels can be challenging to detect using bulk PCR or ELISA. When the concentration of target biomolecules is low, the corresponding detection signal may decrease to a level that cannot be distinguished from the background due to excessive dilution in bulk solutions [ 1 ]. In addition, low-level signals are more prone to the matrix effects making quantification results unreliable.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Digital Biosensing Technology

et al. 2022

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Digital biosensing assays demonstrate remarkable advantages over conventional biosensing systems because of their ability to achieve single-molecule detection and absolute quantification. Unlike traditional low-abundance biomarking screening, digital-based biosensing systems reduce sample volumes significantly to the fL-nL level, which vastly reduces overall reagent consumption, improves reaction time and throughput, and enables high sensitivity and single target detection. This review presents the current technology for compartmentalizing reactions and their applications in detecting proteins and nucleic acids. We also analyze existing challenges and future opportunities associated with digital biosensing and research opportunities for developing integrated digital biosensing systems.

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A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

Cited by 49 publications

References 78 publications

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

All on size-coded single bead set: a modular enrich-amplify-amplify strategy for attomolar level multi-immunoassay

Recent Advances in Digital Biosensing Technology

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