A CURE for Physiological Characterization of Bacterioplankton in Liquid Culture

Lanclos, V. Celeste; Coelho, Jordan T.; Cleveland, Catie S.; Hyer, Alex; McCallum, Mindy C.; Savoie, Emily R.; Kosiba, Scott; Thrash, J. Cameron

doi:10.1128/jmbe.00068-22

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…These take place within credit bearing modules, typically with one instructor supervising multiple students, so are more scalable and inclusive than requiring students to find individual research internships (7)(8)(9). Instructors in a variety of bioscience contexts have designed CUREs to embed research-led experimental work (10)(11)(12)(13). These CUREs vary in their scientific content, but also in the degree of student independence afforded.…”

Section: Introductionmentioning

confidence: 99%

Who Grows There? A Course-based Undergraduate Research Experience to explore the human microbiome through 16S DNA metabarcoding

Sellers,

Freiheit,

Winter

et al. 2024

Preprint

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We describe a two week Course-based Undergraduate Research Experience (CURE) to introduce students to next-generation DNA sequencing, molecular biology methods and a bioinformatic workflow. The CURE is designed to take students with little to no technical and bioinformatic experience through key steps of the protocol through scaffolded laboratory and computational practicals. Our students extract and amplify human microbiome DNA using 16S ribosomal RNA specific primers, then construct a sequencing library for Oxford Nanopore based sequencing. They taxonomically assign the sequencing reads, and determine the ecological community composition using relevant software packages. Our students were able to successfully prepare sequencing libraries and analyse the data to produce relevant figures, demonstrating they met the learning objectives of the CURE. Students identified that they had developed higher level learning as defined by Bloom's taxonomy, and that their confidence in practical work significantly increased as a result of doing the CURE. We share recommendations for implementation of the CURE in undergraduate curricula, and adaptations of the methods for use in schools outreach. Our CURE successfully provides training for students in genetic analysis in an enjoyable and relatively time and cost efficient manner, preparing them for future research or careers in modern molecular biology techniques.

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Section: Introductionmentioning

confidence: 99%

Who Grows There? A Course-based Undergraduate Research Experience to explore the human microbiome through 16S DNA metabarcoding

Sellers,

Freiheit,

Winter

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Employers value effective communication, along with other skills such as teamwork, professionalism, and problem solving 16,17 . Some of these skills are reinforced by the research experience, and CUREs often involve an in‐class or conference presentation as a capstone assignment 5,13,18–22 . Yet, as summarized by Pedwell et al, 23 the CURE literature often lacks details on student preparation for the research presentation.…”

Section: Introductionmentioning

confidence: 99%

Science communication from a course‐based undergraduate research experience

Teter

2023

Biochem Molecular Bio Educ

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Course‐based undergraduate research experiences (CUREs) provide an efficient mechanism to provide many students with an original research project. CUREs often culminate in a capstone poster presentation, but reports on these classes usually focus on the preparation and execution of the project rather than communication of the results. This article summarizes a CURE‐associated research seminar that focuses on developing the communication and interpersonal skills required for the production and presentation of a conference poster. The class is designed to provide students with the tools and confidence for effective communication of their research. From the two class offerings to date, the 18 participating students have received five awards from 19 conference presentations.

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Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance

Wu,

Wang,

et al. 2024

Mol Biol Rep

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A CURE for Physiological Characterization of Bacterioplankton in Liquid Culture

Cited by 3 publications

References 21 publications

Who Grows There? A Course-based Undergraduate Research Experience to explore the human microbiome through 16S DNA metabarcoding

Who Grows There? A Course-based Undergraduate Research Experience to explore the human microbiome through 16S DNA metabarcoding

Science communication from a course‐based undergraduate research experience

Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance

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