In our study, we aimed to assess participants' attitudes and knowledge of the use of acupuncture among those who were 18-65 years old. During the period of May 1–June 1, 2023, a descriptive cross-sectional study was carried out. Between the stated dates, anyone between the ages of 18 and 65 who applied to the Health Sciences University Şişli Hamidiye Etfal Training and Research Hospital's Family Medicine Outpatient Clinics for any reason and who accepted to participate in the study were included. The researcher administered the 24 questioned data form face-to-face with the individuals after obtaining their sociodemographic characteristics, chronic illnesses, knowledge of various TCM treatments and acupuncture. Among the participants, 167 people (73.6%) had information about acupuncture. Acupuncture was one of the most used applications in our survey, and most of the patients who experienced it were referred by physicians. Those who previously received acupuncture treatment knew more about the practice with a statistically significant difference (p=0.004). Those who knew about acupuncture and those who had previously had acupuncture said there was no danger or damage in receiving acupuncture, with a statistically significant difference (p=0.015, p=0.0001, respectively). Although acupuncture is one of the most well-known TCM uses, its use remains in the background. The internet being the most widely used information source limits access to reliable data. Acupuncture should be performed, like all TCM applications, by qualified physicians in authorized centers while taking the outcomes into consideration.