A Custom qPCR Assay to Simultaneously Quantify Human and Microbial DNA
Miriam Foster,
Jennifer A. McElhoe,
Mitchell M. Holland
Abstract:To date, studies on microbial forensics have focused mainly on sequence analysis and generally do not include information on the quantification of and comparison between the human and bacterial DNA present in forensic samples. Knowing the amount of each type of DNA can be important for determining when and how best to employ bacterial DNA analysis, especially when there is insufficient human DNA for successful short tandem repeat (STR) typing. The goal of this work was to develop a quantitative PCR (qPCR) assa… Show more
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