A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex

K-ras modulates the cell cycle via both positive and negative regulatory pathways

“…However, when cells growing in the presence or absence of Tet were subjected to a 24 h serum deprivation, cyclin A expression became undetectable and induction of K-ras failed to upregulate cyclin A. Cyclin A, like other cyclins, forms active complexes with its corresponding CDKs (i.e. CKD2) to drive cell cycle progression (Devoto et al, 1992). To determine whether upregulation of cyclin A by activated K-ras plays a role in cell cycle regulation, cyclin A-associated kinase activity was determined by measuring the ability of the cyclin A immunoprecipitate from total cellular extract to phosphorylate Histone H1 protein.…”

Cell cycle arrest and morphological alterations following microinjection of NIH3T3 cells with Puraα

“…In addition, it is not known whether Pura binds to other Rb-like proteins, such as p107 or p130, which may play a role in cell cycle events (Cao et al, 1992;Cobrinik et al, 1993;Ewen et al, 1991;Hannon et al, 1993;Li et al, 1993;Mayol et al, 1993). In human cells both p107 (Devoto et al, 1992) and p130 (Hannon et al, 1993) may be found in complexes with Cyclin A and CDK2, proteins now believed to associated with Pura (Itoh et al, 1998). In addition to its e ects on DNA replication, several recent reports have now established Pura as a transcription factor (Chepenik et al, 1998;Du et al, 1997;Haas et al, 1995;Herault et al, 1992Herault et al, , 1993Kelm et al, 1997;Krachmarov et al, 1996;Zambrano et al, 1997).…”

Identification of a p130 domain mediating interactions with cyclin A/cdk 2 and cyclin E/cdk 2 complexes

“…Particularly noteworthy among these are the interactions with the E2F/DP family of transcription factors (Cao et al, 1992;Devoto et al, 1992;Helin et al, 1992Helin et al, , 1993Kaelin et al, 1992;Shan et al, 1992;Shirodkar et al, 1992;Cobrinik et al, 1993;Ivey-Hoyle et al, 1993;Lees et al, 1993;Bandara et al, 1994;Bejersbergen et al, 1994;Ginsberg et al, 1994;Buck et al, 1995;Hijmans et al, 1995;Ormondroyd et al, 1995;Sardet et al, 1995;Wu et al, 1995;Zhang and Chellappan, 1995). The E2F transcription factors are thought to control expression of a number of genes involved in cell cycle progression (reviewed in Cobrinik, 1996).…”