A cysteine-selective fluorescent probe for monitoring stress response cysteine fluctuations

Yang, Yang; Zhang, Liangwei; Zhang, Xia; Liu, Shudi; Wang, Yue; Ma, Zhuo; Huh, You; Chen, Lingxin

doi:10.1039/d1cc01110c

Cited by 37 publications

(6 citation statements)

References 40 publications

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“…In the last two decades, there has been a growing trend towards using fluorescent probes to determine the reactive sulfur species in vivo [ 21 , 22 ]. In recent years, studies have provided important information on the use of fluorescent probes to detect LMW thiols e.g., cysteine [ 23 ], as well as antioxidant enzymes, including mammalian TrxR [ 24 ] and Trx [ 25 , 26 ], whereas, there is a very limited understanding of cellular redox homeostasis in bacteria [ 27 ]. Besides the differences in the cell wall/cell membrane between bacterial and mammalian cells, there are great differences in the composition and function of thiol-dependent systems between two types of organisms.…”

Section: Introductionmentioning

confidence: 99%

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria

et al. 2022

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The Trx and Grx systems, two disulfide reductase systems, play critical roles in various cell activities. There are great differences between the thiol redox systems in prokaryotes and mammals. Though fluorescent probes have been widely used to detect these systems in mammalian cells. Very few methods are available to detect rapid changes in the redox systems of prokaryotes. Here we investigated whether Fast-TRFS, a disulfide-containing fluorescent probe utilized in analysis of mammalian thioredoxin reductase, could be used to detect cellular disulfide reducibility in bacteria. Fast-TRFS exhibited good substrate qualities for both bacterial thioredoxin and GSH-glutaredoxin systems in vitro, with Trx system having higher reaction rate. Moreover, the Fast-TRFS was used to detect the disulfide reductase activity in various bacteria and redox-related gene null E. coli. Some glutaredoxin-deficient bacteria had stronger fast disulfide reducibility. The Trx system was shown to be the predominant disulfide reductase for fast disulfide reduction rather than the Grx system. These results demonstrated that Fast-TRFS is a viable probe to detect thiol-dependent disulfide reductases in bacteria. It also indicated that cellular disulfide reduction could be classified into fast and slow reaction, which are predominantly catalyzed by E. coli Trx and Grx system, respectively.

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Section: Introductionmentioning

confidence: 99%

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria

et al. 2022

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“…They play essential roles in a variety of physiological processes, such as maintaining redox homeostasis, protein synthesis, and detoxification. 41 2.3.1 Cys-targeting probes. In the human body, Cys is mainly produced by the methylation of homocysteine through methionine in the liver.…”

Section: Probes Responding To Biothiolsmentioning

confidence: 99%

“…They play essential roles in a variety of physiological processes, such as maintaining redox homeostasis, protein synthesis, and detoxification. 41…”

Section: Drug-induced Liver Injurymentioning

confidence: 99%

Activatable fluorescent probes for early diagnosis and evaluation of liver injury

Lin,

Jiang,

Liu

et al. 2024

Analyst

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“…43 In our lab, we have provided visualization proof that mercury ions could cause the reactive oxygen species to breakout and further change the level of reactive sulfur species. 44,45 Hence, we selected the mercury ion as the typical representative of heavy metal ions for the effect on zinc ion fluctuations. As shown in Figure 6B,E, obvious fluorescent signal decreases were observed when the cells were preincubated with mercury ions, suggesting that zinc ions were reduced under mercury ion stimuli.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Coordination Synergistic-Induced J-Aggregation Enhanced Fluorescent Performance of HBT-Excimers and Imaging Applications

Liu,

Fang,

Jang

et al. 2024

Anal. Chem.

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Developing a novel strategy to improve the optical performances of fluorescent probes is a vital factor in elevating its practical application; viz., novel biocompatible fluorescent probes with excellent multifunctions exhibited unparalleled advantages in probing functions of intracellular molecules to elucidate intracellular events in living systems. Herein, we have successfully constructed a new strategy that aggregation and coordination synergistically induce (2-hydroxylphenyl-benzothiazole) HBT derivatives to form excimers with large red-shifted fluorescence and application for insight into stress-response zinc fluctuations in living systems. We have synthesized four HBT-based derivatives and deeply investigated the response mechanism by fluorescent spectral studies, demonstrating that probes 3 and 4 showcased large red shifts in emission wavelength due to J-aggregation. More interestingly, the fluorescence of probe 4 was significantly enhanced in the presence of a zinc ion, suggesting that zinc coordination synergistically induced J-aggregation. Probe 4 was successfully applied to image zinc fluctuations in different models of living systems, proving that this probe is a powerful tool to unveil the relationship between invasive stress and diseases by monitoring endogenous zinc fluctuations.

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A cysteine-selective fluorescent probe for monitoring stress response cysteine fluctuations

Abstract: Rare studies provided evidences for real-time monitoring stress response cysteine fluctuations. Here, we have successfully designed and synthesized cysteine-selective fluorescent probe 1 to monitor stress response Cys fluctuations, firstly provided...

Cited by 37 publications

References 40 publications

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria

Activatable fluorescent probes for early diagnosis and evaluation of liver injury

Coordination Synergistic-Induced J-Aggregation Enhanced Fluorescent Performance of HBT-Excimers and Imaging Applications

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