A Cytoplasmic Membrane-like Fraction from Cells of the Ehrlich Mouse Ascites Carcinoma

Rajam, P. C.; Jackson, Anne-Louise

doi:10.1038/1811670a0

Cited by 8 publications

(1 citation statement)

References 2 publications

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“…Boell and Nachmansohn found it possible to isolate the myelin sheath, contaminated by connective tissue, of giant squid axon by dissecting the nerve and squeezing out the axoplasm (8). By microdissection of formalin-fixed amoebae the pellicle has been isolated (9). Membranes of mouse ascites tumor cells have recently been isolated by differential centrifugation after sonic disruption, however, adherent cytoplasmic particles contaminate this preparation (10).…”

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The Isolation of a Cell Membrane Fraction From Rat Liver

Neville

1960

The Journal of Cell Biology

488

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A procedure is described for isolating cell membranes from rat liver homogenates. 20 gm. of rat liver was homogenized in a Dounce homogenizer in ice cold water buffered to pH 7.5 with NaHCO3, rupturing all of the cells and most nuclei. The diluted homogenate was filtered through cheesecloth to remove precipitated nucleoprotein and centrifuged at 1500 g, 10 minutes, to sediment a crude membrane fraction. The membrane containing sediment was recentrifuged 3 times in conical tubes (1220 g, 10 minutes), the top layer of the 2-layered sediment being retained. Flotation in a sucrose solution d = 1.22 freed the preparation from contaminating cell fragments and nuclear membranes not previously disintegrated. The floating material ∼0.4 ml. was quite homogeneous and consisted of thin amorphous membranes. Electron micrographs revealed numerous double profiles similar in shape and dimensions to apposed liver cell membranes in intact tissue.

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confidence: 99%