A decoupler-free simple paper microchip capillary electrophoresis device for simultaneous detection of dopamine, epinephrine and serotonin

Roychoudhury, Appan; Francis, Kevin; Patel, Jay; Jha, Sandeep Kumar; Basu, Suddhasatwa

doi:10.1039/d0ra03526b

Cited by 85 publications

(26 citation statements)

References 45 publications

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“…For example, abnormal quantities of urinary oxalate and calcium can provide an indication of kidney stones [86], while excessive quantities of urinary glucose and protein may be a sign of diabetes [87]. Similarly, high levels of urinary serotonin can be an early indicator of melancholia [88]. The literature thus contains many proposals for lab-on-paper platforms based on urine samples for the detection of diabetes, kidney disease, liver and heart function disorders, pregnancy, drugs, heavy metals, and so on.…”

Section: Application Of Lab-on-paper Platforms To Human Disease Diagnosis Using Urine Samplesmentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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In recent years, microfluidic lab-on-paper devices have emerged as a rapid and low-cost alternative to traditional laboratory tests. Additionally, they were widely considered as a promising solution for point-of-care testing (POCT) at home or regions that lack medical infrastructure and resources. This review describes important advances in microfluidic lab-on-paper diagnostics for human health monitoring and disease diagnosis over the past five years. The review commenced by explaining the choice of paper, fabrication methods, and detection techniques to realize microfluidic lab-on-paper devices. Then, the sample pretreatment procedure used to improve the detection performance of lab-on-paper devices was introduced. Furthermore, an in-depth review of lab-on-paper devices for disease measurement based on an analysis of urine samples was presented. The review concludes with the potential challenges that the future development of commercial microfluidic lab-on-paper platforms for human disease detection would face.

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Section: Application Of Lab-on-paper Platforms To Human Disease Diagnosis Using Urine Samplesmentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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“…Therefore, the rapid and accurate determination of dopamine concentration in vivo is of great significance. At present, there are many methods for the detection of dopamine, such as colorimetry [ 7 ], spectrophotometry [ 8 ], capillary electrophoresis [ 9 ], electrochemiluminescence [ 10 ], high-performance liquid chromatography [ 11 ], and fluorescence [ 12 ]. However, most analytical techniques have some shortcomings, such as high-performance liquid chromatography (HPLC), which requires large equipment and is expensive, and fluorescence analysis, which has a complex detection process and poor selectivity and effectiveness [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Research on Temperature-Switched Dopamine Electrochemical Sensor Based on Thermosensitive Polymers and MWCNTs

et al. 2023

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A temperature-controlled electrochemical sensor was constructed based on a composite membrane composed of temperature-sensitive polymer poly (N-isopropylacrylamide) (PNIPAM) and carboxylated multi-walled carbon nanotubes (MWCNTs-COOH). The sensor has good temperature sensitivity and reversibility in detecting Dopamine (DA). At low temperatures, the polymer is stretched to bury the electrically active sites of carbon nanocomposites. Dopamine cannot exchange electrons through the polymer, representing an “OFF” state. On the contrary, in a high-temperature environment, the polymer shrinks to expose electrically active sites and increases the background current. Dopamine can normally carry out redox reactions and generate response currents, indicating the “ON” state. In addition, the sensor has a wide detection range (from 0.5 μM to 150 μM) and low LOD (193 nM). This switch-type sensor provides new avenues for the application of thermosensitive polymers.

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“…Different analytical methods in this field have focused on 5-HT detection, including high-performance liquid chromatography (HPLC) (Chau., Patel, 2009), fluorescence (Hernández-Mendoza et al, 2020), chemiluminescence (Kong et al, 2021), and capillary-electrophoresis (Roychoudhury et al, 2020). However, all these methods involve some disadvantages, such as the use of a large volume of solvents incompatible with the environment, long working time, expensive devices, and complicated experimental processes.…”

Section: Introductionmentioning

confidence: 99%

A novel electrochemical sensor based on metal nanoparticles and molecularly imprinted polymer nanocomposite with biological applications

Shaker

Vardini

Es’haghi

et al. 2022

MGPB

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Metal nanoparticles trapped in a biopolymer composite due to electrical conductivity properties improve electrochemical sensors with biomedical and environmental applications. The study aims are to design a novel molecularly imprinted polymer (MIP) composite based on magnetic graphene oxide (Fe3O4@GO) modified silica (SiO2) and gold nanoparticles (AuNPs) to electrochemical detect serotonin (5-hydroxytryptamine, 5-HT). A suitable amount of 5-HT is effective on motivational functions and the environment because it is a serotonergic neurotransmitter. But the desired nanocomposite may have a relatively low recognition, therefore must be in choosing the type of functional monomer be careful. In this regard, the design of the electrochemical sensor began by synthesis of Fe3O4@GO-SiO2@AuNPs nanocomposite. Then, MIP electropolymerization was carried out by using p-aminothiophenol (PATP)-functionalized Fe3O4@GO-SiO2@AuNPs nanocomposite in the presence of 5HT as a template molecule. Electrochemical polymerization of MIP nanocomposite was developed using cyclic voltammetry (CV) and the electrochemical properties of 5-HT were studied use differential pulse voltammetry (DPV) technology in the 5HT solution. After optimization of preparation and measurement conditions on the designed sensor, the 5HT concentration range is 0.1 μM to 10 μM linearly, and the detection limit was 1 × 10-5 μM (S / N = 3). The wide concentration range and low detection limit were presented metal nanoparticles functionalized MIP with appropriate functional monomer have a great effect on the performance of the sensor. Furthermore, PATP-functionalized metal nanoparticles increase the conductivity and recognition of the prepared MIP electrochemical sensor to the quantification of 5-HT in biological samples with high selectivity and recovery.

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A decoupler-free simple paper microchip capillary electrophoresis device for simultaneous detection of dopamine, epinephrine and serotonin

Abstract:
This paper demonstrates a simplified configuration for capillary electrophoresis-amperometric detection using paper microfluidic chip for separation and simultaneous detection of three clinically relevant neurochemicals without using any decouplers.

Cited by 85 publications

References 45 publications

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Research on Temperature-Switched Dopamine Electrochemical Sensor Based on Thermosensitive Polymers and MWCNTs

A novel electrochemical sensor based on metal nanoparticles and molecularly imprinted polymer nanocomposite with biological applications

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A decoupler-free simple paper microchip capillary electrophoresis device for simultaneous detection of dopamine, epinephrine and serotonin

Abstract: This paper demonstrates a simplified configuration for capillary electrophoresis-amperometric detection using paper microfluidic chip for separation and simultaneous detection of three clinically relevant neurochemicals without using any decouplers.

Cited by 85 publications

References 45 publications

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Research on Temperature-Switched Dopamine Electrochemical Sensor Based on Thermosensitive Polymers and MWCNTs

A novel electrochemical sensor based on metal nanoparticles and molecularly imprinted polymer nanocomposite with biological applications

Contact Info

Product

Resources

About

Abstract:
This paper demonstrates a simplified configuration for capillary electrophoresis-amperometric detection using paper microfluidic chip for separation and simultaneous detection of three clinically relevant neurochemicals without using any decouplers.