A deeper insight into the sialome of male and female Ochlerotatus triseriatus mosquitoes

Lu, Stephen; Martín-Martín, Inés; Ribeiro, José M. C.; Calvo, Eric

doi:10.1016/j.ibmb.2022.103800

Cited by 6 publications

(11 citation statements)

References 87 publications

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“…Together, they represent 41.4% of all secreted transcripts and ~25.4% of all secreted proteins ( Table 2 ). These values are similar to those found in other sialomes [ 37 , 38 , 40 , 76 ] and perhaps reflect a hidden pharmacological potential existing within the salivary glands of hematophagous vectors.…”

Section: Resultssupporting

confidence: 87%

“…To assess the quality of our assembly, we performed the BUSCO analysis of completeness using the Insecta database as a reference. The current dataset displayed a completeness of 80.7% (76.5% single and 4.2% duplicated), 2.9% of fragmentation and 16.4% of missing sequences, which is within the range of previous genomic and transcriptome assemblies [36][37][38]. For comparison, we conducted the same analysis using the C. felis genome assembly [20] and observed a completeness of 94% (49.5% single and 44.5% duplicated) with 0.5% of fragmented and 5.5% of missing sequences.…”

Section: Overall Description Of C Felis Sialotranscriptome and Sialop...supporting

confidence: 75%

“…In the horsefly Tabanus yao antigen 5-like proteins isolated from the salivary glands interfere with thrombus formation, angiogenesis and platelet aggregation [ 74 , 75 ]. Interestingly, in mosquitoes, antigen 5-like molecules have been reported in the sialome of both males and females, even though some transcripts were 10- and 100-fold upregulated in females [ 38 , 76 ]. Together these results suggest that members of this family have multiple roles in vector biology, including blood acquisition.…”

Section: Resultsmentioning

confidence: 99%

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Revisiting the sialome of the cat flea Ctenocephalides felis

Danchenko

Macaluso

et al. 2023

PLoS ONE

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The hematophagous behaviour emerged independently in several instances during arthropod evolution. Survey of salivary gland and saliva composition and its pharmacological activity led to the conclusion that blood-feeding arthropods evolved a distinct salivary mixture that can interfere with host defensive response, thus facilitating blood acquisition and pathogen transmission. The cat flea, Ctenocephalides felis, is the major vector of several pathogens, including Rickettsia typhi, Rickettsia felis and Bartonella spp. and therefore, represents an important insect species from the medical and veterinary perspectives. Previously, a Sanger-based sialome of adult C. felis female salivary glands was published and reported 1,840 expressing sequence tags (ESTs) which were assembled into 896 contigs. Here, we provide a deeper insight into C. felis salivary gland composition using an Illumina-based sequencing approach. In the current dataset, we report 8,892 coding sequences (CDS) classified into 27 functional classes, which were assembled from 42,754,615 reads. Moreover, we paired our RNAseq data with a mass spectrometry analysis using the translated transcripts as a reference, confirming the presence of several putative secreted protein families in the cat flea salivary gland homogenates. Both transcriptomic and proteomic approaches confirmed that FS-H-like proteins and acid phosphatases lacking their putative catalytic residues are the two most abundant salivary proteins families of C. felis and are potentially related to blood acquisition. We also report several novel sequences similar to apyrases, odorant binding proteins, antigen 5, cholinesterases, proteases, and proteases inhibitors, in addition to putative novel sequences that presented low or no sequence identity to previously deposited sequences. Together, the data represents an extended reference for the identification and characterization of the pharmacological activity present in C. felis salivary glands.

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Section: Resultssupporting

confidence: 87%

Section: Overall Description Of C Felis Sialotranscriptome and Sialop...supporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

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Revisiting the sialome of the cat flea Ctenocephalides felis

Danchenko

Macaluso

et al. 2023

PLoS ONE

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“…The levels mapped reads were similar between our male and female samples (64.5% ± 0.6),with exception of one female sample (F1), which presented only 49.4% of mapped reads, indicating a possible bias with this sample. Despite this discrepancy, the values of mapped reads observed here are within range of other sialome from blood feeding arthropods [ 16 , 29 ] and indicate that no major bias is present in our pipeline. It is noteworthy that the remaining unmapped reads could be potentially from 5` and 3` UTRs or putative CDS that we failed to identify due to lack of homology to previous deposited sequences, lack of a putative signal peptide or having an ORF with less than 150 nucleotides.…”

Section: Resultsmentioning

confidence: 50%

“…Pools of 50 pairs of female salivary glands and 100 pairs of male salivary glands were stored at − 80 °C until RNA extraction. More male than female salivary glands were used to account for their overall lower total RNA amount [ 15 , 16 ]. Three biological replicates of male and female samples were collected.…”

Section: Methodsmentioning

confidence: 99%

A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes

Martín-Martín

Ribeiro

et al. 2023

BMC Genomics

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Introduction During evolution, blood-feeding arthropods developed a complex salivary mixture that can interfere with host haemostatic and immune response, favoring blood acquisition and pathogen transmission. Therefore, a survey of the salivary gland contents can lead to the identification of molecules with potent pharmacological activity in addition to increase our understanding of the molecular mechanisms underlying the hematophagic behaviour of arthropods. The southern house mosquito, Culex quinquefasciatus, is a vector of several pathogenic agents, including viruses and filarial parasites that can affect humans and wild animals. Results Previously, a Sanger-based transcriptome of the salivary glands (sialome) of adult C. quinquefasciatus females was published based on the sequencing of 503 clones organized into 281 clusters. Here, we revisited the southern mosquito sialome using an Illumina-based RNA-sequencing approach of both male and female salivary glands. Our analysis resulted in the identification of 7,539 coding DNA sequences (CDS) that were functionally annotated into 25 classes, in addition to 159 long non-coding RNA (LncRNA). Additionally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and/or pathogen transmission. Conclusion Together, these findings represent an extended reference for the identification and characterization of the proteins containing relevant pharmacological activity in the salivary glands of C. quinquefasciatus mosquitoes.

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Native structure of mosquito salivary protein uncovers domains relevant to pathogen transmission

et al. 2023

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Female mosquitoes inject saliva into vertebrate hosts during blood feeding. This process transmits mosquito-borne human pathogens that collectively cause ~1,000,000 deaths/year. Among the most abundant and conserved proteins secreted by female salivary glands is a high-molecular weight protein called salivary gland surface protein 1 (SGS1) that facilitates pathogen transmission, but its mechanism remains elusive. Here, we determine the native structure of SGS1 by the cryoID approach, showing that the 3364 amino-acid protein has a Tc toxin-like Rhs/YD shell, four receptor domains, and a set of C-terminal daisy-chained helices. These helices are partially shielded inside the Rhs/YD shell and poised to transform into predicted transmembrane helices. This transformation, and the numerous receptor domains on the surface of SGS1, are likely key in facilitating sporozoite/arbovirus invasion into the salivary glands and manipulating the host’s immune response.

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A deeper insight into the sialome of male and female Ochlerotatus triseriatus mosquitoes

Cited by 6 publications

References 87 publications

Revisiting the sialome of the cat flea Ctenocephalides felis

Revisiting the sialome of the cat flea Ctenocephalides felis

A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes

Native structure of mosquito salivary protein uncovers domains relevant to pathogen transmission

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