A Descriptive Analysis of Urinary ESBL-Producing-Escherichia coli in Cerdanya Hospital

Gaviria, Lorena Patrícia; Montsant, Lourdes; Azuaje, Carlos; González-Díaz, Aida; Horcajada, Juan Pablo; Cáceres, Enrique Limón; Viñas, Miguel; Espinal, Paula; Fusté, Ester

doi:10.3390/microorganisms10030488

Cited by 8 publications

(8 citation statements)

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“…The prevalence of resistance against antibiotics ceftazidime, cefotaxime, aztreonam and ceftriaxone were considerably high. Approximately, it is in agreement with studies in Ethiopia, Nigeria and Cyprus [ 21 , 27 , 28 ] and higher than the results in Sri Lanka, US and Canada [ 23 – 25 , 29 , 30 ]. Furthermore, resistance towards cefotaxime was more frequent than against ceftazidime, in accordance with the results reported by Gaviria et al (2022) in Cerdanya [ 30 ].…”

Section: Discussionsupporting

confidence: 91%

“…Approximately, it is in agreement with studies in Ethiopia, Nigeria and Cyprus [ 21 , 27 , 28 ] and higher than the results in Sri Lanka, US and Canada [ 23 – 25 , 29 , 30 ]. Furthermore, resistance towards cefotaxime was more frequent than against ceftazidime, in accordance with the results reported by Gaviria et al (2022) in Cerdanya [ 30 ]. Frequency rate of resistance against ciprofloxacin was found in approximately half of our isolates.…”

Section: Discussionsupporting

confidence: 91%

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High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran

et al. 2023

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Background Urinary tract infection (UTI) is one of the most prevalent infectious diseases with worldwide health threatening. Antimicrobial resistant strains of Escherichia coli (E. coli) are a common cause of UTI which were identified as a treatment challenge. This study aimed to assay the prevalence of common β-lactam resistance genes including blaTEM, blaSHV, blaCTX-M and blaCMY and phenotypic resistance to commonly used β-lactam and fluoroquinolone antibiotics in UTIs. These factors were evaluated in various phylogenetic groups (phylotypes) of E. coli isolates. Real-time PCR was applied to detect β-lactam resistance genes and conventional PCR was used to determine the phylotypes. Phenotypic resistance against β-lactams (ceftazidime, cefotaxime, aztreonam and ceftriaxone) and fluoroquinolones (ciprofloxacin) were identified by the disc diffusion technique. The ability of extended spectrum β-lactamases (ESBLs) production in E. coli isolates was detected using the combined disc diffusion method. Results The prevalence of resistance genes were 89.6% for blaTEM, 44.3% for blaCTX-M, 6.6% for blaSHV and 0.9% for blaCMY. The two high prevalent phylotypes were B2 (29.2%) and D (17.9%) followed by E (14.1%), F (9.4%), C (6.6%) and 10.3% of isolates were unknown in phylotyping. Disc diffusion results showed high prevalence of antibiotic resistance to cefotaxime (88.6%), aztreonam (83%), ceftireaxon (77.3%), ceftazidime (76.4%) and ciprofloxacin (55.6%). Totally, 52.8% of isolates were found as phenotypical ESBL-producers. Conclusions This study’s results confirmed an explosion of antibiotic resistance amongst E. coli isolates from UTI against β-lactams and fluoroquinolones. Findings explain the necessity of deep changes in quantity and quality of drug resistance diagnosis and antibiotic therapy strategies. More studies are suggested to better and confident evaluations.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran

et al. 2023

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“…Moreover, WGS revealed that this strain carried bla NDM gene alongside bla CTX-M-15 , bla TEM-1 , aadA2 , sul1 , gyrA , parC , parE , and dfrA12 genes [ 48 ]. A previous study identified plasmid replicons ( IncFIB , IncFII , IncFIA , IncI1 , IncX1 , IncFIC , and Col ) that could carry resistance and virulence genes in ST-405 [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Escherichia coli Causing Urinary Tract Infections Through Next-Generation Sequencing: A Comprehensive Analysis of Serotypes, Sequence Types, and Antimicrobial and Virulence Genes

Kandi,

Shahapur,

Suvvari

et al. 2024

Cureus

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Introduction An enormous increase in antimicrobial resistance (AMR) among bacteria isolated from human clinical specimens contributed to treatment failures. Increased surveillance through next-generation sequencing (NGS) or whole genome sequencing (WGS) could facilitate the study of the epidemiology of drug-resistant bacterial strains, resistance genes, and other virulence determinants they are potentially carrying. Methods This study included 30 Escherichia coli ( E . coli ) isolates obtained from patients suffering from urinary tract infections (UTIs) attending Prathima Institute of Medical Sciences, Karimnagar, India. All bacterial isolates were identified, and antimicrobial susceptibility patterns were determined through conventional microbiological techniques and confirmed by automated systems. All the isolates were investigated using NGS to identify genes coding for resistance, such as extended-spectrum beta-lactamases (ESBLs), metallo-beta-lactamases, and virulence genes. Multilocus sequence typing (MLST) was used to understand the prevalent strain types, and serotyping was carried out to evaluate the type of O (cell wall antigen) and H (flagellar antigen) serotypes carried by the isolates. Results The conventional antimicrobial susceptibility testing revealed that 15 (50%) isolates were resistant to imipenem (IPM), 10 (33.33%) were resistant to amikacin (AK), 13 (43.33%) were resistant to piperacillin-tazobactam (PTZ), 17 (56.66%) were resistant to cephalosporins, and 14 (46.66%) were resistant to nitrofurantoin (NIT). Among the isolates, 26 (86.66%) had revealed the presence of multiple antibiotic-resistant genes with evidence of at least one gene coding for beta-lactamase resistance. There was a high prevalence of bla CTX-M (19/30, 63.33%) genes, followed by bla TEM and bla OXA-1 . The bla NDM-5 gene was found in three isolates (3/30, 10%). The virulence genes identified in the present study were iutA , sat , iss , and papC , among others. The E . coli serotype found predominantly belonged to O25:H4 (5, 16.66%), followed by O102:H6 (4, 13.33%). A total of 16 MLST variants were identified among the examined samples. Of the MLST-based sequence types (STs) identified, ST-131 (7, 23.33%) was the predominant one, followed by ST-167 (3, 10%) and ST-12 (3, 10%). Conclusions The study results demonstrated that the E . coli strains isolated from patients suffering from UTIs potentially carried antimicrobial resistance and virulence genes and belonged to different strain types based on MLST. Careful evaluation of bacterial strains using molec...

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“…In fact, ESBLs are beta-lactamases able to provide bacterial resistance by hydrolyzing various antibiotics: penicillins, first-, second-, and third generation cephalosporins, as well as aztreonam [ 9 ]. For this reason, infections caused by ESBL producer bacteria require the administration of different antibiotic classes, such as fluoroquinolones—that may produce adverse effects due to their known toxicities—or carbapenems, which should be considered last resort drugs that should be spared and can be used only in hospital settings [ 7 ] [ 10 ]. Alarmingly, the increasing rate of Enterobacterales carbapenem resistance (mainly due to carbapenemases’ production), adds additional complications to the treatment of both ABP and CPB [ 4 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Oral Fosfomycin Formulation in Bacterial Prostatitis: New Role for an Old Molecule-Brief Literature Review and Clinical Considerations

Marino

Stracquadanio

Bellanca

et al. 2022

Infectious Disease Reports

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Bacterial prostatitis infections are described as infections that are difficult-to-treat, due to prostate anatomic characteristics along with clinical difficulty in terms of diagnosis and management. Furthermore, the emergence of multidrug resistant (MDR) bacteria, such as extended-spectrum beta-lactamase (ESBL) producer Escherichia coli, also representing the main causative pathogen in prostatitis, poses major problems in terms of antibiotic management and favorable clinical outcome. Oral fosfomycin, an antibiotic commonly used for the treatment of uncomplicated urinary tract infections (UTIs), has been recently evaluated for the treatment of bacterial prostatitis due to its favorable pharmacokinetic profile, its activity against MDR gram-positive and gram-negative bacteria, safety profile, and multiple synergic effect with other antibiotics as well as the low resistance rate. This review addresses fosfomycin pharmacokinetics and pharmacodynamics and discusses the latest clinical evidence on its clinical use to treat acute and chronic bacterial prostatitis in hospitalized patients and in outpatients. As described in several reports, oral fosfomycin may represent a valid therapeutic option to treat susceptible germs commonly causing prostatitis, such as E. coli and other Enterobacterales as well as Enterococcus faecium, even as a first-line regimen in particular clinical settings (patients with previous treatment failure, with allergies or outpatients). Stronger data from further studies, including randomized controlled trials, would be helpful to establish the proper dosage and specific indications.

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A Descriptive Analysis of Urinary ESBL-Producing-Escherichia coli in Cerdanya Hospital

Cited by 8 publications

References 59 publications

High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran

High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran

Molecular Characterization of Escherichia coli Causing Urinary Tract Infections Through Next-Generation Sequencing: A Comprehensive Analysis of Serotypes, Sequence Types, and Antimicrobial and Virulence Genes

Oral Fosfomycin Formulation in Bacterial Prostatitis: New Role for an Old Molecule-Brief Literature Review and Clinical Considerations

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