The COVID-19 pandemic posed a major global health challenge. Preventive measures against the spread of COVID-19 require the involvement of all sections of society. Knowledge and attitude towards COVID-19 preventive measures influence human practices. We describe the knowledge, attitude and practice (KAP) of COVID-19 preventive measure in Bhutan. This was a cross-sectional survey using multistage-cluster sampling involving participants from all 20 districts of the country. The knowledge was assessed using 12 items, attitude using 6 Likert items and practice using 10 items. There was total of 1708 respondents. The mean knowledge was 10.7, (SD = 1.5; range 0–12); 86.38% had good knowledge, 10.95% had average knowledge, 2.69% had poor knowledge. The common sources of knowledge were television (84.9%) and family and friends (74.7%). Those younger than 30 years were associated with good knowledge. The fear of contracting COVID-19 was reported by 96% and 86.4% agreed that appropriate preventive measures can help control the spread of COVID-19. Nearly all the respondents (97%) wore mask while going out and majority practiced good hand hygiene (87.9%) and proper cough etiquette (84.1%). The knowledge on COVID-19 preventive measures was good and the majority held positive attitudes and practices.