A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the Vf gene

Gardiner, S. E.; Bassett, H.; Noiton, Dominique; Bus, Vincent G. M.; Hofstee, M. E.; White, A. G.; Ball, R. D.; Forster, R. L. S.; Rikkerink, Erik H. A.

doi:10.1007/s001220050306

Cited by 17 publications

(24 citation statements)

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“…The success rate of 0.6% can be considered quite low; however, this result does not differ greatly from previous studies which identified RAPD markers linked to the dominant resistant gene Vf (Koller et al 1994;Yang and Krüger 1994;Tartarini 1996;Gardiner et al 1996). This can be explained by the use of heterozygous resistant bulks, which reduce the probability of detecting polymorphism by 50% (Melchinger 1990).…”

Section: Discussioncontrasting

confidence: 49%

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

et al. 2004

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Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance gene Vbj originating from the crab apple Malus baccata jackii. Using bulk segregant analysis, three RAPD markers linked to Vbj were first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be codominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype-phenotype incongruence (GPI plants) plants, a linkage map was calculated. Vbj mapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6 cM from CH05e03 and at 3.9 cM from T6-SCAR. Without the removal of the GPI plants, Vbj was placed 15 cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated.

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Section: Discussioncontrasting

confidence: 49%

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

et al. 2004

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“…Rvi6 (Vf) as well as 'Honeycrisp' progeny populations. This agrees with Gardiner et al (1996), who rejected the hypothesis that the range in Rvi6 phenotypes was a result of the loss of closely linked R genes as large portions of the donor genome (M. floribunda 821) were still intact around the locus after several generations of introgression into breeding lines. "Modifying genes" may also cause variations in the resistance expression of broad spectrum resistance loci to the extent that they are assigned to different resistance classes for a given R gene that is observed in a progeny population (Chevalier et al 1991;Durel et al, 2004).…”

Section: Discussionsupporting

confidence: 82%

“…The symptoms range from: no lesions (0), HR (1), chlorotic lesions (2), necrotic/chlorotic lesions with slight sporulation (3a-weak resistance), necrotic/chlorotic lesions with sporulation (3b-weak susceptibility), and lesions with high sporulation (4) (Chevalier et al 1991). The differences observed in a progeny population among those containing the R gene is the result of isolate virulence and minor or modifying genes that are also segregating in the host population (Lamb and Hamilton, 1969;Williams and Kuc, 1969;Gessler, 1992;Gardiner et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the defence response to Venturia inaequalis in ‘Honeycrisp’ apple, its ancestors, and progeny

et al. 2014

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“…They were used to study sexrelated differences in recombination rates (Hemmat et al 1994) to locate genes of interest like scab resistance major genes (Gardiner et al 1996;Gianfranceschi et al 1996;Maliepaard et al 1998;Vinatzer et al 2001;Xu and Korban 2002;Hemmat et al 2003;Belfanti et al 2004;Gygax et al 2004;Bus et al 2005) and quantitative trait loci (QTLs; Durel et al 2003;Liebhard et al 2003b;Calenge et al 2004), rosy leaf curling aphid resistance gene (Roche et al 1997), the self-incompatibility locus , QTLs for growth and development (Conner et al 1998;Kenis and Keulemans 2007;Segura et al 2007), QTLs for fruit skin color (Cheng et al 1996), fruit quality (King et al 2000;Costa et al 2005) and vitamin C content (Davey et al 2006). Liebhard et al (2003a) constructed a saturated map for the apple genome, which consisted of different types of markers (amplified fragment length polymorphism (AFLP), random amplification of polymorphic DNA (RAPD), simple sequence repeats (SSRs), SCAR).…”

Section: Introductionmentioning

confidence: 99%

Construction of an integrated consensus map of the apple genome based on four mapping populations

N’Diaye

Weg

Kodde

et al. 2008

Tree Genetics & Genomes

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An integrated consensus genetic map for apple was constructed on the basis of segregation data from four genetically connected crosses (C1=Discovery × TN10-8, C2=Fiesta × Discovery, C3=Discovery × Prima, C4= Durello di Forli × Fiesta) with a total of 676 individuals using CarthaGene® software. First, integrated female-male maps were built for each population using common femalemale simple sequence repeat markers (SSRs). Then, common SSRs over populations were used for the consensus map integration. The integrated consensus map consists of 1,046 markers, of which 159 are SSR markers, distributed over 17 linkage groups reflecting the basic chromosome number of apple. The total length of the integrated consensus map was 1,032 cM with a mean distance between adjacent loci of 1.1 cM. Markers were proportionally distributed over the 17 linkage groups (χ 2 = 16.53, df=16, p=0.41). A non-uniform marker distribution was observed within all of the linkage groups (LGs). Clustering of markers at the same position (within a 1-cM window) was observed throughout LGs and consisted predominantly of only two to three linked markers. The four integrated female-male maps showed a very good colinearity in marker order for their common markers, except for only two (CH01h01, CH05g03) and three (CH05a02z, NZ02b01, Lap-1) markers on LG17 and LG15, respectively. This integrated consensus map provides a framework for performing quantitative trait locus (QTL) detection in a multi-population design and evaluating the genetic background effect on QTL expression.

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A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the Vf gene

Cited by 17 publications

References 0 publications

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

Characterization of the defence response to Venturia inaequalis in ‘Honeycrisp’ apple, its ancestors, and progeny

Construction of an integrated consensus map of the apple genome based on four mapping populations

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