A detailed phenotypic and genotypic description of Pseudomonas strain OX1

Cladera, Aina Maria; Sepúlveda-Torres, Lycely del C.; Valens-Vadell, Maria; Meyer, Jean‐Marie; Lalucat, Jorge; Garcı́a-Valdés, Elena

doi:10.1016/j.syapm.2005.11.007

Cited by 20 publications

(16 citation statements)

References 25 publications

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“…Only the amplicons for the glycerol facilitator uptake protein (glpF) and malate dehydrogenase (mdh) were useful for distantly related strains in the genus. Other studies have successfully applied MLSA to Ensifer, Pseudomonas, and Vibrio species (3,21,36), not only for differentiation on the intraspecies level, but also between several distantly related species. The development of such a genus-wide MLSA scheme for Rhodopirellula can make use of the glpF and mdh primer pairs, but additional genomic information from several strains is needed to develop a Rhodopirellula genus-wide MLSA.…”

Section: Discussionmentioning

confidence: 99%

Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis

Winkelmann

Jaekel

Meyer

et al. 2010

Appl Environ Microbiol

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In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species.The biogeography of microorganisms describes the habitat size of the species and the distribution of microorganisms on Earth. The experimental approaches depend on the focus of the studies. Habitats are often analyzed by environmental microbiologists with genetic-fingerprinting techniques, with up to 200 bands or fragments representing the whole community. Although the taxonomic resolution of these operational taxonomic units (OTUs) is limited, the studies revealed a community biogeography (22). Medical microbiologists analyze the alleles of housekeeping genes of microorganisms to gain insight into the epidemiology of pathogens, the population biogeography (2). This strain-specific, fine-scale taxonomic resolution within a species is well suited to observance of recent dispersal events. At the species level, multilocus sequence typing (MLST) and analysis (MLSA), which were developed for intraspecies and intragenus specific studies, respectively, consist of the sequences of several (at least seven) housekeeping gene fragments concatenated to an approximately 5-kilobase alignment (17). Recent MLSA studies revealed its applicability to marine isolates and the analysis of biogeographic patterns: Alteromonas macleodii isolates could be grouped in an epipelagic and an abyssal clade (6), and strains of Pseudomonas aeruginosa were genetically well separated into groups of coastal and oceanic origin (8). However, for Salinibacter ruber strains, biogeographical distinctness was not resolved in an MLSA study but showed allopatry in a metabolic analysis (31). Several studies used MLSA together with DNA-DNA hybridization (DDH) for the delineation of new species, e.g., for Vibrio and Ensifer spp. (20,36).In the biogeography of microorganisms, the experimental proof of a local genetic evolution was first revealed at sample sites...

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Section: Discussionmentioning

confidence: 99%

Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis

Winkelmann

Jaekel

Meyer

et al. 2010

Appl Environ Microbiol

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“…It has been intensively studied due to its significant phenotypic characteristics. However, when strain OX1 was characterized taxonomically in detail, it turned out to be a member of the P. corrugata phylogenetic branch (73). Pseudomonas sp.…”

Section: Differentiation From Other Speciesmentioning

confidence: 99%

“…However, all strains can use nitrate as an alternative electron acceptor and can carry out oxygen-repressible denitrification. Denitrification may be delayed or may appear only after serial transfers in nitrate media under semiaerobic conditions (73,340). Oxidative degradation of aromatic compounds involves the participation of mono-and dioxygenases.…”

Section: Phenotypic Propertiesmentioning

confidence: 99%

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

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552

418

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SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

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“…Furthermore, Psuedomonas sp. OX1 has the ability to metabolize NO 43. A description of the reactivity of potential target proteins other than those intended for NO metabolism is therefore relevant to the global management of nitric oxide within the organism.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Iron Dinitrosyl Species Formed in the Reaction of Nitric Oxide with a Biological Rieske Center

et al. 2010

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Reactions of nitric oxide with cysteine-ligated iron-sulfur cluster proteins typically result in disassembly of the iron-sulfur core and formation of dinitrosyl iron complexes (DNICs). Here we report the first evidence that DNICs also form in the reaction of NO with Rieske-type [2Fe-2S] clusters. Upon treatment of a Rieske protein, component C of toluene/o-xylene monooxygenase (ToMOC) from Pseudomonas sp. OX1, with a slight excess of NO (g) or NO-generators S-nitroso-N-acetyl-D,L-pencillamine (SNAP) and diethylamine NONOate (DEANO), the absorbance bands of the [2Fe-2S] cluster are epxtinguished and replaced by a new feature that slowly grows in at 367 nm. Analysis of the reaction products by EPR, Mössbauer, and NRVS spectroscopy reveals that the primary product of the reaction is a thiolate-bridged diiron tetranitrosyl species, [Fe2(μ-SCys)2(NO)4] having a Roussin's red ester (RRE) formula, and that mononuclear DNICs account for only a minor fraction of nitrosylated iron. Reduction of this RRE reaction product with sodium dithionite produces the one-electron reduced Roussin's red ester (rRRE) having absorbtions at 640 and 960 nm. These results demonstrate that NO reacts readily with Rieske centers in protein and suggest that dinuclear RRE species, not mononuclear DNICs, may be the primary iron dinitrosyl species responsible for the pathological and physiological effects of nitric oxide in such systems in biology.

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A detailed phenotypic and genotypic description of Pseudomonas strain OX1

Cited by 20 publications

References 25 publications

Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis

Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis

Biology of Pseudomonas stutzeri

Characterization of Iron Dinitrosyl Species Formed in the Reaction of Nitric Oxide with a Biological Rieske Center

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