A detection system for fluorescence EXAFS at the SRS wiggler beam line

Baines, J. T.; Garner, C. David; Hasnain, S.S.; Morrell, C.

doi:10.1016/0168-9002(86)90154-3

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…A premonochromator toroidal mirror was used to focus the beam at the sample position, not reflecting radiation with wavelength < 1.2 Á and rejecting harmonics. The experiment on 9.2 was carried out at ~77 K, with an Oxford Instruments liquid helium fluorescence EXAFS cryostat and a setup with individual electronics for every detector as described elsewhere (Baines et al, 1986). The double-crystal order-sorting monochromator (Greaves et al, 1983) was set to 50% harmonic rejection.…”

Section: Methodsmentioning

confidence: 99%

Zinc environment in sheep liver sorbitol dehydrogenase

Feiters¹,

Jeffery²

1989

Biochemistry

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The extended X-ray absorption fine structure (EXAFS) associated with the zinc K-absorption edge has been recorded for sorbitol dehydrogenase. It is interpreted in terms of one cysteine sulfur among the ligands to the active site zinc atom. Simulations of the EXAFS based on the presence of two such sulfurs are less satisfactory, and comparison with the EXAFS of such systems points to the presence of only one sulfur ligand in sorbitol dehydrogenase. These results provide evidence that sorbitol dehydrogenase does not have the characteristic one water, one His, two Cys arrangement of ligands to the active site zinc found in the homologous alcohol dehydrogenases and are consistent with the one water, one His, one Cys, one Glu ligand arrangement of the proposed model of sorbitol dehydrogenase [Eklund, H., Horjales, E., Jörnvall, H., Brändén, C.-I., & Jeffery J. (1985) Biochemistry 24, 8005-8012]. Evidence for the correctness of the model is also evidence for validity of predictive techniques used in constructing the model, i.e., computer graphics fitting of the amino acid sequence to the crystallographically derived structure of a different but homologous protein.

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Section: Methodsmentioning

confidence: 99%

Zinc environment in sheep liver sorbitol dehydrogenase

Feiters¹,

Jeffery²

1989

Biochemistry

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“…Four Nal detectors with Zr filters of appropriate thickness (cf. Baines et al, 1986) were employed. An Mo foil standard, placed in series after the sample, and with its X-ray absorption recorded simultaneously with the data by an additional ion chamber, was employed for calibration and for precise alignment of individual spectra.…”

Section: X-ray Spectramentioning

confidence: 99%

Information from e.x.a.f.s. spectroscopy on the structures of different forms of molybdenum in xanthine oxidase and the catalytic mechanism of the enzyme

Turner

Bray

Diakun

1989

Biochemical Journal

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X-ray spectroscopy was used to provide further information on the structure of the molybdenum centre of xanthine oxidase. Earlier work was confirmed and two states of the enzyme, not reported on by previous workers, were studied. One of these was the complex of the enzyme with pyridine-3-carboxaldehyde, in which most of the metal is in the Mo(V) state, giving the e.p.r. signal known as Inhibited. The other was the complex with the inhibitor alloxanthine, with the metal as Mo(IV). For both complexes clear evidence was obtained that an oxo ligand of molybdenum was present, but not a sulphido ligand. This information complements structural information on these complexes already available from e.p.r. spectroscopy, and has permitted us to revise and refine the structures previously proposed. The mechanism of action of the enzyme is discussed in the light of the present findings on the persistence of the oxo group in the reduced enzyme complexes, as well as of related evidence [George & Bray (1988) Biochemistry 27, 3603-3609] for an oxo group in the catalytic intermediate that gives the Mo(V) e.p.r. signal known as Very Rapid.

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Application of EXAFS to biochemical systems

Hasnain

1988

Topics in Current Chemistry

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A detection system for fluorescence EXAFS at the SRS wiggler beam line

Cited by 5 publications

References 10 publications

Zinc environment in sheep liver sorbitol dehydrogenase

Zinc environment in sheep liver sorbitol dehydrogenase

Information from e.x.a.f.s. spectroscopy on the structures of different forms of molybdenum in xanthine oxidase and the catalytic mechanism of the enzyme

Application of EXAFS to biochemical systems

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