This study aimed to explore the dynamics and implications of cross-functional collaborations in Nigeria’s healthcare sector, particularly in the context of global health crises and the unique challenges faced by the nation. Utilizing a systematic approach, data were extracted from selected sources using a structured form, capturing study objectives, methodology, key findings, and implications specific to Nigeria’s healthcare landscape. The extracted data underwent thematic analysis, identifying patterns and themes that provide a nuanced understanding of healthcare collaborations in Nigeria. The findings underscore the transformative power of cross-functional collaborations, especially evident during the COVID-19 pandemic, where diverse disciplines converged towards a shared objective. The study also highlighted the critical role of patent and proprietary medicine vendors (PPMVs) in Nigeria’s healthcare provision, emphasizing the need for their continuous training and regulation. Collaborative initiatives, such as responses to disease outbreaks and the push for Universal Health Coverage (UHC), were identified as pivotal strategies to address the multifaceted challenges facing Nigeria’s health system. In conclusion, cross-functional collaborations in Nigeria’s healthcare sector present a promising avenue to address systemic challenges, drive innovation, and improve patient outcomes. Recommendations include strengthening regulatory frameworks, prioritizing technological integration, enhancing interdisciplinary training, and addressing systemic barriers to effective collaboration.