A differential stain for the anterior lobe of the hypophysis

Cleveland, Rucker; Wolfe, J. M.

doi:10.1002/ar.1090510317

Cited by 63 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, immunostaining was abolished when the antisera were omitted or preabsorbed with the corresponding antigens, while cross-absorption of the antisera with the non-corresponding peptide had no effect. For histological and histochemical study, the sections were stained with: (1) Herlant's alcian blue-periodic acid-schiff (PAS)-orange G (Herlant II;Herlant, 1960); (2) Gomori's chromium-haematoxylin-phloxin (CHP) (Gomori, 1941); (3) Cleveland-Wolfe trichrome (CW) (Cleveland & Wolfe, 1932); (4) Azan-Heidenhein (Azan) (Koneff, 1938); (5) lead haematoxylin (PbH) (Solcia et al, 1969) with or without PAS or (6) PAS-haematoxylin (PASH).…”

Section: Methodsmentioning

confidence: 99%

Identification and localization of hormone‐producing cells in the pituitary of male Padogobius martensi(Pisces, Gobiidae): a histochemical and immunocytochemical study

Cinquetti

Dramis

2006

Journal of Fish Biology

View full text Add to dashboard Cite

The adenohypophysial cell types of the adult male freshwater goby Padogobius martensi were identified and localized using histochemical and immunocytochemical techniques. Lactotropes were arranged in an irregular mass which occupied a major portion of the rostral pars distalis (RPD). Lead haematoxylin (PbH) stained the corticotropes selectively. These cells formed large clusters or islets between the neurohypophysial branches penetrating the RPD and the lactotropes. In addition, few isolated or clustered corticotropes were observed in the proximal pars distalis (PPD). Corticotropes showed a moderate immunoreactivity to synthetic anti-human adrenocorticotropic hormone 1-24 but a strong immunoreactivity with antiserum to human a-melanocyte-stimulating hormone, which so far has not been observed in any other teleosts. Of the two mouse monoclonal antibodies to the human thyrotropin b subunit used, one failed to bind the thyrotropes, while the other stained selectively but very weakly cells in the dorsal-anterior part of the proximal pars distalis (PPD) indicating that thyrotropes were probably located in this region. Somatotropes were arranged mainly in a multicellular layer bordering the neurohypophysial processes penetrating the PPD, but some were isolated throughout the PPD. The antiserum to rainbow trout Oncorhynchus mykiss gonadotropin I b subunit bound to gonadotropes located in the central and ventral part of the PPD, and along the external periphery of the pars intermedia (PI). The cells reacting to the antiserum to rainbow trout gonadotropin II b subunit largely showed the same distribution as gonadotropes I, but some were also found isolated or in small clusters between somatolactotropes and melanotropes at the interface PI/neurohypophysis (NH) and throughout the PI, as well as intermingled with thyrotropes in the dorsal-anterior part of the PPD, and with lactotropes and corticotropes in the RPD. These results indicate the presence of two types of both gonadotropins and gonadotropes in P. martensi, but do not exclude some degree of gonadotropin I and gonadotropin II colocalization in the same cells. A distribution of gonadotropes II throughout the adenohypophysis similar to that found in P. martensi has not yet been observed in other teleosts; it suggests the possibility of local interactions between these cells and all the other adenohypophysial cell types. The PI contained somatolactotropes, which were distributed along the neurohypophysial border, and melanotropes which were arranged in clusters mostly located further away from the neural tissue. Melanotropes did not react to PbH and showed positive immunostaining to both anti-human ACTH 1-24 and anti-human a-MSH.

show abstract

Section: Methodsmentioning

confidence: 99%

Identification and localization of hormone‐producing cells in the pituitary of male Padogobius martensi(Pisces, Gobiidae): a histochemical and immunocytochemical study

Cinquetti

Dramis

2006

Journal of Fish Biology

View full text Add to dashboard Cite

show abstract

“…All pituitaries were fixed by the method of Severinghaus (1932), of Cleveland and Wolf e (1932), and were stained after the same authors' methods. Paraffin sections were cut serially at 3,~ in the sagittal plane.…”

Section: Methodsmentioning

confidence: 99%

Cytological Studies of the Anterior Pituitary in the Broody Fowl

Yasuda

1953

Proceedings of the Japan Academy

View full text Add to dashboard Cite

“…Mercury was removed from the tissues fixed in the Bouin-Hollande fixative by immersing the slides in iodine (1%) in 70% alcohol, followed by sodium thiosulfate (about 5%) and then rinsing in tap water and distilled water. In order to visualize the thyroid gland development, the sectioned larvae were stained using Cleveland-Wolfe trichrome staining (Cleveland and Wolfe 1932). The tissue sections were stained in Erlich's haematoxylin for 5 min, then ''blued'' by a short immersion in alkaline alcohol to stain nuclei, rinsed in tap water and distilled water, followed by staining in 1% aqueous erythrosine for 5 min.…”

Section: Histologymentioning

confidence: 99%

Thyroid and pituitary gland development from hatching through metamorphosis of a teleost flatfish, the Atlantic halibut

et al. 2005

View full text Add to dashboard Cite

Fish larval development, not least the spectacular process of flatfish metamorphosis, appears to be under complex endocrine control, many aspects of which are still not fully elucidated. In order to obtain data on the functional development of two major endocrine glands, the pituitary and the thyroid, during flatfish metamorphosis, histology, immunohistochemistry and in situ hybridization techniques were applied on larvae of the Atlantic halibut (Hippoglossus hippoglossus), a large, marine flatfish species, from hatching through metamorphosis. The material was obtained from a commercial hatchery. Larval age is defined as day-degrees (D°=accumulated daily temperature from hatching). Sporadic thyroid follicles are first detected in larvae at 142 D°(27 days post-hatch), prior to the completion of yolk sack absorption. Both the number and activity of the follicles increase markedly after yolk sack absorption and continue to do so during subsequent development. The larval triiodothyronine (T 3 ) and thyroxine (T 4 ) content increases, subsequent to yolk absorption, and coincides with the proliferation of thyroid follicles. A second increase of both T 3 and T 4 occurs around the start of metamorphosis and the T 3 content further increases at the metamorphic climax. Overall, the T 3 content is lower than T 4 . The pituitary gland can first be distinguished as a separate organ at the yolk sack stage. During subsequent development, the gland becomes more elongated and differentiates into neurohypophysis (NH), pars distalis (PD) and pars intermedia (PI). The first sporadic endocrine pituitary cells are observed at the yolk sack stage, somatotrophs (growth hormone producing cells) and somatolactotrophs (somatolactin producing cells) are first observed at 121 D°( 23 days post-hatch), and lactotrophs (prolactin producing cells) at 134 D°(25 days post-hatch). Scarce thyrotrophs are evident after detection of the first thyroid follicles (142 D°), but coincident with a phase in which follicle number and activity increase (260 D°). The somatotrophs are clustered in the medium ventral region of the PD, lactotrophs in the anterior part of the PD and somatolactotrophs are scattered in the mid and posterior region of the pituitary. At around 600 D°, coinciding with the start of metamorphosis, somatolactotrophs are restricted to the interdigitating tissue of the NH. During larval development, the pituitary endocrine cells become more numerous. The present data on thyroid development support the notion that thyroid hormones may play a significant role in Atlantic halibut metamorphosis. The time of appearance and the subsequent proliferation of pituitary somatotrophs, lactotrophs, somatolactotrophs and thyrotrophs indicate at which stages of larval development and metamorphosis these endocrine cells may start to play active regulatory roles.

show abstract

A differential stain for the anterior lobe of the hypophysis

Abstract: A considerable number of investigations have been published which have demonstrated that the anterior lobe of the hypophysis plays an important rBle in the physiology of the female reproductive system. Some of these studies have shown not only that the hypophysis is an important part of

Cited by 63 publications

References 6 publications

Identification and localization of hormone‐producing cells in the pituitary of male Padogobius martensi(Pisces, Gobiidae): a histochemical and immunocytochemical study

Identification and localization of hormone‐producing cells in the pituitary of male Padogobius martensi(Pisces, Gobiidae): a histochemical and immunocytochemical study

Cytological Studies of the Anterior Pituitary in the Broody Fowl

Thyroid and pituitary gland development from hatching through metamorphosis of a teleost flatfish, the Atlantic halibut

Contact Info

Product

Resources

About