A diffusional analysis of the temperature sensitivity of the Mg2+-induced rise of chlorophyll fluorescence from pea thylakoid membranes

Rubin, B.T.; Barber, James; Paillotin, Guy; Chow, Wah Soon; Yamamoto, Yasutake

doi:10.1016/0005-2728(81)90186-9

Cited by 33 publications

(13 citation statements)

References 21 publications

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“…4 and 6, may not be real. This value for the lateral mobility of PS I is higher by three orders of magnitude than the previously estimated lateral diffusion of photosystem II pigment protein complex (22). From an analysis of the temperature dependence of the fluorescence changes, induced by the addition of MgCl2, assumed to result from PS II particle aggregation (22), obtained estimates of 2 10-12_3 10-" cm2s-' for the lateral diffusion coefficient of PS II (in the temperature range of 10-30°C).…”

Section: Emd (1)contrasting

confidence: 59%

Electric field-induced lateral mobility of photosystem I in the photosynthetic membrane

Brumfeld

Miller

Korenstein

1989

Biophysical Journal

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Electrophoretic movement of photosystem I (PS I) along the photosynthetic membrane of hypotonically swollen thylakoid vesicles was studied by analyzing the electric field-stimulated delayed luminescence (electrophotoluminescence) emitted from PS I. The electrophoretic mobility was inferred from the differences in electrophotoluminescence (EPL) of the photosynthetic vesicles in presence and absence of trains of low amplitude (<80 V/cm) prepulses of 1 ms duration at 4 ms spacing. The average apparent electric mobility, determined from the time course of EPL increase on one hemisphere or its decrease on the other one, as function of prepulse length and intensity was of the order of 3 . 10(-5) cm(2)V(-1)s(-1). The assymetric distribution of the PS I reached a steady state when the diffusional, electrostatic, and elastic forces balanced the electrophoretic driving force. A lateral diffusion coefficient of approximately 5 . 10(-9) cm(2)s(-1) was found for the PS I complex from the diffusional relaxation after cessation of the electric field pulse train. Experimental conditions such as concentration, temperature, and viscosity of the aqueous solution were not critical for the effect. Between 23 and 150 electron charges per moving particle were estimated from the measured electrophoretic mobility.

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Section: Emd (1)contrasting

confidence: 59%

Electric field-induced lateral mobility of photosystem I in the photosynthetic membrane

Brumfeld

Miller

Korenstein

1989

Biophysical Journal

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“…The lipid and protein diffusion coefficients in the thylakoid membranes obtained in the past studies are nicely summarized in a recent review ( Kirchhoff, 2014 ). Both the earlier and recent studies show rather small diffusion coefficients in the overall thylakoids ( Rubin et al, 1981 ; Kirchhoff et al, 2008 ), reflecting the highly crowded nature of the membranes, but information on the local membrane fluidity is not available yet.…”

Section: Membrane Lipids and Membrane Fluiditymentioning

confidence: 85%

Quality Control of Photosystem II: The Mechanisms for Avoidance and Tolerance of Light and Heat Stresses are Closely Linked to Membrane Fluidity of the Thylakoids

Yamamoto

2016

Front. Plant Sci.

117

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When oxygenic photosynthetic organisms are exposed to excessive light and/or heat, Photosystem II is damaged and electron transport is blocked. In these events, reactive oxygen species, endogenous radicals and lipid peroxidation products generated by photochemical reaction and/or heat cause the damage. Regarding light stress, plants first dissipate excessive light energy captured by light-harvesting chlorophyll protein complexes as heat to avoid the hazards, but once light stress is unavoidable, they tolerate the stress by concentrating damage in a particular protein in photosystem II, i.e., the reaction-center binding D1 protein of Photosystem II. The damaged D1 is removed by specific proteases and replaced with a new copy produced through de novo synthesis (reversible photoinhibition). When light intensity becomes extremely high, irreversible aggregation of D1 occurs and thereby D1 turnover is prevented. Once the aggregated products accumulate in Photosystem II complexes, removal of them by proteases is difficult, and irreversible inhibition of Photosystem II takes place (irreversible photoinhibition). Important is that various aspects of both the reversible and irreversible photoinhibition are highly dependent on the membrane fluidity of the thylakoids. Heat stress-induced inactivation of photosystem II is an irreversible process, which may be also affected by the fluidity of the thylakoid membranes. Here I describe why the membrane fluidity is a key to regulate the avoidance and tolerance of Photosystem II on environmental stresses.

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“…Fractionation at pH 6.3 appears to protect the donor system of PS2 from the effects of the detergent, although the 1.5 h period allowed for the incubation of chloroplasts in the stacking medium may also be important in this respect, and could allow increased separation of PS2 from PSI in the thylakoid membrane [6,17]. The fractionation procedure described has been applied to pea, lettuce, and spinach chloroplasts with equal success.…”

Section: Light Intensity Wni2mentioning

confidence: 99%

Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity

1983

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Detergent-treatment of higher plant thylakoids with Triton X-100 at pH 6.3 has been used to purify a PS2 fraction with very high rates of oxygen evolution (> 1000 pmol. mg chl-' . h-l). A photosynthetic unit size of about 300 chlorophyll (chl) molecules has been determined by optical methods, suggesting an average turnover time for PS2 of about 2 ms. The donor system for P680+ is particularly well preserved in the preparation, as judged by P680+ reduction kinetics, the detection by EPR of Signal 11~ and the presence of the high potential form of cytochrome b-559 (at a ratio of 1: 1 with the reaction centre). Photosystem 2Oxygen evolution P680+

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A diffusional analysis of the temperature sensitivity of the Mg2+-induced rise of chlorophyll fluorescence from pea thylakoid membranes

Cited by 33 publications

References 21 publications

Electric field-induced lateral mobility of photosystem I in the photosynthetic membrane

Electric field-induced lateral mobility of photosystem I in the photosynthetic membrane

Quality Control of Photosystem II: The Mechanisms for Avoidance and Tolerance of Light and Heat Stresses are Closely Linked to Membrane Fluidity of the Thylakoids

Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity

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