A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Abstract

Traditional diagnosis of infectious diseases relies on advanced analyzers in central hospitals which is not sufficient for rapid control of epidemics, especially in resource-limited areas, raising the importance of the...

Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip

“…The polymerase chain reaction (PCR) technology, which enables in vitro amplification of target nucleic acid sequences, , is widely recognized as the gold standard in the molecular biology technique due to its sensitivity and accuracy . Compared to bulky and expensive PCR thermal cyclers, , the point-of-care testing (POCT) devices based on the microfluidic chip technology (also known as lab-on-a-chip) have garnered significant attention. − These devices offer advantages such as reduced reagent volume, rapid reaction time, easy-to-use, and portability.…”

“… 3 Compared to bulky and expensive PCR thermal cyclers, 4 , 5 the point-of-care testing (POCT) devices based on the microfluidic chip technology (also known as lab-on-a-chip) have garnered significant attention. 6 − 8 These devices offer advantages such as reduced reagent volume, rapid reaction time, easy-to-use, and portability.…”

A point-of-care testing platform for on-site identification of genetically modified crops

“…27 However, the extraction of nucleic acids, amplification of DNA fragments and detection of exogenous genetic modifications require bulky instruments and trained personnel. 28,29 Due to the large volume of crops and the complexity of the environment in customs, markets and etc. , 30 achieving on-site testing and decreasing the turn-around time are critically important.…”

Laser-induced graphene-based digital microfluidics (gDMF): a versatile platform with sub-one-dollar cost

“…4,5 With these advantages, DMF has become a promising platform for a wide variety of biological and biomedical applications, among which point-of-care testing (POCT) is one of the most important fields. 6–8 To date, various POCT applications have been demonstrated on DMF devices, including detection of nucleic acids, 8–11 immunoassay, 12–15 and cell analysis. 16–18 However, for nearly all of these application scenarios, the DMF chips are not only required to be fully functional to address complex tasks, but disposable to avoid cross-contamination.…”