A digital platform for One-Pot signal enhanced foodborne pathogen detection based on mesophilic argonaute-driven polydisperse microdroplet reactors and machine learning

Wen, Junping; Han, Minjie; Feng, Niu; Chen, Guoxun; Jiang, Feng; Lin, Jianhan; Chen, Yiping

doi:10.1016/j.cej.2024.148845

Cited by 11 publications

References 46 publications

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Cutting‐Edge Technology for Early Intervention in Myocardial Infarction: Portable Fingertips‐Based Immunobiosensor

Yu,

Wu,

Gao

et al. 2024

Advanced Sensor Research

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Early intervention in acute myocardial infarction can minimize myocardial damage and improve patient survival. Herein, a low‐cost device‐free portable immunobiosensing platform for flexible monitoring of immediate myocardial infarction is reported. CuS‐Pt nanofragments (CuS‐Pt NFs) with high photothermal conversion efficiency (≈26.41%) are synthesized by liquid‐phase polarity‐mediated synthesis. The CuS NFs are loaded in situ with platinum (Pt) nanoreactors using a solvothermal reduction strategy, which is employed to enhance the efficiency of gas production. The resulting CuS‐Pt nanocatalysts are encapsulated within liposomes for signal cascade amplification. Specifically, cardiac troponin I (cTn I), a target biomarker in serum, is captured on pre‐modified microtiter plates and formed into a classical sandwich model. The thermo‐chemically kinetically enhanced CuS‐Pt reactor is released through a one‐step chemical treatment and transferred to a closed gas generator. Under the excitation of a near‐infrared laser emitter, the internal pressure in the gas generator device increases with time and drives the carbon quantum dot solution in the connected hose. The moving distance shows a correlation with the target concentration. This work provides a new implementation for the development of low‐cost, efficient pressure immunosensors without the requirement of a readout device.

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Cutting‐Edge Technology for Early Intervention in Myocardial Infarction: Portable Fingertips‐Based Immunobiosensor

Yu,

Wu,

Gao

et al. 2024

Advanced Sensor Research

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Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Zhou,

Zhao,

Wen

et al. 2024

Advanced Science

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Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, and convenient detection of viable bacteria is an effective method for reducing infections and illness outbreaks. Here, an unsupervised learning–assisted and surface acoustic wave–interdigital transducer‐driven nano‐lens holography biosensing platform is developed for the ultrasensitive and amplification‐free detection of viable bacteria. The monitoring device integrated with the nano‐lens effect can achieve the holographic imaging of polystyrene microsphere probes in an ultra‐wide field of view (∽28.28 mm2), with a sensitivity limit of as low as 99 nm. A lightweight unsupervised learning hologram processing algorithm considerably reduces training time and computing hardware requirements, without requiring datasets with manual labels. By combining phage–mediated viable bacterial DNA extraction and enhanced CRISPR–Cas12a systems, this strategy successfully achieves the ultrasensitive detection of viable Salmonella in various real samples, demonstrating enhanced accuracy validated with the qPCR benchmark method. This approach has a low cost (∽$500) and is rapid (∽1 h) and highly sensitive (∽38 CFU mL−1), allowing for the amplification‐free detection of viable bacteria and emerging as a powerful tool for food safety inspection and clinical diagnosis.

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Microfluidics in smart food safety

Gong,

Lin

2024

Advances in Food and Nutrition Research

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A digital platform for One-Pot signal enhanced foodborne pathogen detection based on mesophilic argonaute-driven polydisperse microdroplet reactors and machine learning

Cited by 11 publications

References 46 publications

Cutting‐Edge Technology for Early Intervention in Myocardial Infarction: Portable Fingertips‐Based Immunobiosensor

Cutting‐Edge Technology for Early Intervention in Myocardial Infarction: Portable Fingertips‐Based Immunobiosensor

Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Microfluidics in smart food safety

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