A Direct (Non-Competitive) Immunoassay for Gentamicin Residues with an Optical Biosensor

Haasnoot, W.; Verheijen, R.

doi:10.1080/09540100120055701

Cited by 29 publications

(17 citation statements)

References 4 publications

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“…However, with an LOD (determined as three times the average response of the blank milk) of 10 ng ml -1 , the assay was still useful for the detection far below the MRL of 200 ng ml -1 . These results were similar to earlier results obtained with the direct BIA for gentamicin using the same milk sample (Haasnoot & Verheijen, 2001). By diluting the milk sample with buffer (five times), the difference between the curves in buffer and milk was reduced, supporting the suggestion of matrix interference.…”

Section: Direct Biassupporting

confidence: 92%

“…Lower concentrations of STREP resulted in reduced signals (see Figure 1). These results were comparable with the results obtained with the direct BIA for gentamicin (Haasnoot & Verheijen, 2001). The antibodies were regenerated by the injection of 5 ml 5 mM-HCl at a flow rate of 20 ml min -1 .…”

Section: Direct Biassupporting

confidence: 87%

“…This was confirmed during an investigation of interactions between immobilized thrombin and its inhibitors in solution (Karlsson et al, 2000). The application of this biosensor for the direct detection of the LMWC gentamicin (466 Da) in milk was described previously (Haasnoot & Verheijen, 2001). Using sensor chip immobilized anti-gentamicin MAbs, gentamicin could be detected directly.…”

Section: Introductionmentioning

confidence: 79%

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Direct Versus Competitive Biosensor Immunoassays for the Detection of (Dihydro)Streptomycin Residues in Milk

Haasnoot¹,

Loomans²,

Cazemier³

et al. 2002

Food and Agricultural Immunology

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A monoclonal antibody (MAb) against dihydrostreptomycin (4G8) was developed and its performance compared with a previously developed MAb against streptomycin (4E2) in biosensor immunoassays (BIAs) using a surface plasmon resonance (SPR)-based biosensor (BIACORE 3000). Direct BIAs for the detection of dihydrostreptomycin (DHS; 583 Da) and streptomycin (STREP; 581 Da) were developed by immobilising the MAbs on the sensor chip (CM5). These direct BIAs were compared with competitive inhibition BIAs, using a STREPprotein conjugate immobilized on the chip. The sensitivities of the direct and competitive BIAs for both drugs in buffer were comparable (10-20 ng ml -1 at 50% binding or inhibition). With milk, interferences, probably due to the nonspecific binding of proteins to the sensor chips, were observed in both BIAs. These interferences could be largely reduced using ultra filtration (UF) as sample pre-treatment. Another option was the use of a reference flow channel to correct for nonspecific binding. Using this option with five times diluted milk, MAb 4G8 was found to be suited for the direct BIA of both drugs with a limit of detection (LOD) of 20 ng ml -1 and both MAbs could be applied in the competitive BIA format with similar LODs.

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Section: Direct Biassupporting

confidence: 92%

Section: Direct Biassupporting

confidence: 87%

Section: Introductionmentioning

confidence: 79%

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Direct Versus Competitive Biosensor Immunoassays for the Detection of (Dihydro)Streptomycin Residues in Milk

Haasnoot¹,

Loomans²,

Cazemier³

et al. 2002

Food and Agricultural Immunology

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“…Vibrio cholerae detection via its toxin was suggested by Swanson et al by combining the natural receptor for the toxin, the glycolipid GM1, with a fluorescence energy transfer optical transducer [168]. Antibiotic and other drug residue analysis in food samples have been published, such as the detection of penicillin in milk using photometric immunosensors [169], chloramphenicol was quantified in bile, urine, and meat [170], a variety of veterinary drugs, such as clenbuterol and ethinyl estradiol in urine, and enrofloxacin in milk were detected using an eight-channel surface plasmon resonance immunosensor [171], Hassnoot et al described the use of the Biacore system for the identification of gentamicin in milk samples [172], and compare direct versus competitive strategies for (dihydro)streptomycin immunoassays [173].…”

Section: Antibody and Receptor-based Biosensorsmentioning

confidence: 99%

Biosensors for environmental pollutants and food contaminants

Baeumner

2003

Analytical and Bioanalytical Chemistry

216

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This review article provides an overview of the most recent literature on biosensors for environmental pollutants and food contaminants. Due to the large number of publications, only papers published between 2000 and January 2003 were considered. Also, while not all of the published literature could be reviewed here, over 200 references are cited to provide a good overview of research undertaken in the last two years. Older publications are covered by a number of earlier review articles. This article provides an introduction into the field including specific consideration of the application areas, describes the typical biosensor assay format used, and is subsequently structured according to the biorecognition elements used (i.e., nucleic acids, enzymes, whole cells, tissue and whole organisms, antibodies and receptors, and biomimetic materials). In addition, a section on microbiosensing systems is provided. Since only very few microbiosensors with applications in environmental and food systems have been published, enabling technology is also covered in this article.

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“…Besides, some other interesting applications have been published, dealing with global conformational transitions in human phenylalanine hydroxylase [91], examining peptides from the GH loop of the foot-and-mouth disease virus [92], measuring gentamicin residues [93], and determining penicillin residues in milk [94].…”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 99%