A direct organogenesis protocol from shoot segments of Solanum tuberosum cv. Monalisa

Abstract

Transgenic technology is an excellent alternative for improvement of crop production and disease free crops such as potato, which is one of the most important crops worldwide. One of the first steps to apply the transgenic technique is the establishment of an efficient plant regeneration protocol. This is a limiting step in this kind of study, since most protocols are species-specific and some of them do not adequately respond to in vitro culture or present low regeneration rates. The objective of this study was to stablish an efficient regeneration protocol of Solanum tuberosum cv. Monalisa from internodes explants. This work is important since most in vitro protocols are based on shoots. Twenty five treatments were performed, with each treatment being composed of six internodes in flasks containing MS medium supplemented with a fixed concentration of zeatin riboside (3 mg.L-1) (ZEA), varying concentrations of naphthaleneacetic acid (0 to 1 mg. L-1) (NAA), and gibberellic acid (0 to 3 mg.L-1) (GA 3). The treatment composed of ZEA, 0.05 mg.L-1 of NAA, and 0.10 mg.L-1 of GA 3 was considered the best for shoot regeneration from potato internodes. The study was able to establish a specific regeneration protocol for Monalisa cultivar. This result can be very useful since it is possible to obtain plants from internode, without the requirement of meristematic regions, enabling the obtainment of a higher number of plants.

High frequency direct shoot regeneration from Kazakh commercial potato cultivars

“…Hence, in the current study, we evaluated direct shoot regeneration from four potato genotypes of Kazakhstan breeding using two different explants. Our results are in agreement with studies demonstrating that GA 3 isan important plant hormone with the ability to induce a number of responses, including direct shoot regeneration ( Ibragimova et al, 2018 ; Roest & Bokelmann, 1976 ), and suggesting that the combination of auxins and cytokinins in the presence of GA 3 enhances shoot regeneration from explants ( Campos et al, 2016 ; Kumlay, 2014 ).…”

“…Many studies have reported that often most of the tissue culture process is cultivar-specific. It is, therefore, necessary to establish a specific protocol for each genotype studied ( Campos et al, 2016 ; Tara, Krishnaprasad & Veena, 2017 ; Yadav & Sticklen, 1995 ).…”

“…Callus formation and organogenesis have been studied for potato cultivar Almera, and TDZ at a concentration of 5.0 mg/l was shown to be the most active cytokinin for plant regeneration ( Mutasim, Khadiga & Rasheid, 2010 ). A specific regeneration protocol has been established for cultivar Monalisa, and the best PGR combinations for shoot regeneration from potato internodes were considered to be zeatin, NAA, and GA 3 at concentrations of 3 mg/l, 0.05 mg/l, and 0.10 mg/l, respectively ( Campos et al, 2016 ). An effective protocol has also been developed for cultivars Arnova, Burren, Provento, and Riviera ( AL-Hussaini, Yousif & AL-Ajeely, 2015 ).…”

The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

“…Shoot organogenesis protocol, a pre-requisite for genetic manipulation of plants has been well documented in potato but is complicated by use of two step procedure (Yee et al 2001, Yasmin et al 2003, Elaleem et al 2009, Molla et al 2011, Rezende et al 2013, Ghosh et al 2014, Abdelaleem 2015, Campos et al 2016. Moreover, genotype specific response of potato cultivars to plant growth regulators (PGRs) has also been reported by many researchers (Khokan et al 2009, Pal et al 2011, Wheeler et al 1985.…”

“…In potato, an important rabi food crop, shoot organogenesis is extensively studied using different explants such as leaf, internode and tuber discs (Yasmin et al 2003, Elaleem et al 2009, Molla et al 2011, Rezende et al 2013, Ghosh et al 2014, Abdelaleem 2015, Campos et al 2016, Kaur et al 2017). Most of the reports are mainly focussed on Plant Growth regulators (PGRs), but, studies on the role of other factors such as gelling agents, light intensity, medium pH, silver nitrate etc.…”