A Dispersive Liquid–Liquid Microextraction and Chiral Separation of Carvedilol in Human Plasma Using Capillary Electrophoresis

Hamidi, Samin; Soltani, Somaieh; Jouyban, Abolghasem

doi:10.4155/bio.15.51

Cited by 28 publications

(11 citation statements)

References 40 publications

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“…Various methods were reported for the determination of tramadol including chromatography [11][12][13][14][15][16][17][18], electrophoresis [19,20], and electrochemical methods [21]. Biological matrices containing a large amount of proteins, salts, and lipids are required to be cleaned-up prior to being coupled with the majority of analytical devices due to the clogging risk of the column [22][23][24]. LLE and SPE are commonly employed as sample preparation methods in the extraction of target analyte from aqueous or biological samples [25,26].…”

Section: Introductionmentioning

confidence: 99%

Mixed hemimicelle magnetic dispersive solid‐phase extraction using carbon‐coated magnetic nanoparticles for the determination of tramadol in urine samples

Taghvimi

Hamidi

Javadzadeh

2018

J of Separation Science

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Ionic liquid carbon‐coated magnetic nanoparticles were successfully applied as an adsorbent in a mixed hemimicelle magnetic dispersive solid‐phase extraction method for the determination of tramadol from urine samples coupled with high‐performance liquid chromatography with UV‐vis detection. The significant parameters affect the extraction efficiency including type and amount of adsorbent, sample volume, pH, ionic strength, type and amount of elution solvent, time of extraction and desorption, time of ionic liquid loading on the adsorbent and stirring rate were studied and optimized. The proposed method provided a fast, straightforward, environmentally friendly and adsorbent recyclable approach for tramadol analysis. The linear range for the tramadol determination was from 100 to 1500 ng/mL. Precisions and accuracies were within 6%. The applicability of the proposed method in clinical trial was tried successfully on determination of tramadol in addicted subjects under tramadol therapy. The mean percent recovery of the patient samples was 94%. The results proved that the proposed method could be applied in clinical and forensic laboratories for determination of tramadol from biological urine samples.

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Section: Introductionmentioning

confidence: 99%

Mixed hemimicelle magnetic dispersive solid‐phase extraction using carbon‐coated magnetic nanoparticles for the determination of tramadol in urine samples

Taghvimi

Hamidi

Javadzadeh

2018

J of Separation Science

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“…DLLME and USAEME not only reduce consumption of organic solvent, but also reduce equilibration time because of the increased contact area between two immiscible phases. Recently, some DLLME-based methods combined with spectrofluorimetry, 26 high performance liquid chromatographic-ultraviolet (HPLC-UV), 27 capillary electrophoretic-ultraviolet (CE-UV) 28,29 have been reported for determination of drugs in human plasma and urine samples. In a previous work, the applicability of the DLLME method as an efficient microextraction technique for bioanalysis was proposed and its results were compared with those of USAEME.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Sajedi-Amin

Asadpour‐Zeynali

Khoubnasabjafari

et al. 2016

J. Braz. Chem. Soc.

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A feasible, rapid, sensitive and reliable dispersive liquid-liquid microextraction (DLLME) and ultrasonic assisted liquid-liquid microextraction (USAEME) combined with high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for analysis of bosentan in human plasma and urine samples. All validation tests were reported according to FDA guidelines. Since chromatographic methods are precise, accurate and provide excellent separation are increasingly being used in determination of trace amount of drugs in biological goals. So the optimized experimental extraction factors from our previous work were used to evaluate HPLC-UV method for decreasing the limit of quantifications (LOQs) to cover the therapeutic range. Simple centrifugation of urine samples was sufficient prior to microextraction procedure, while acetonitrile protein precipitation provides sample clean up for plasma samples. The suggested method was linear (correlation coefficient R 2 > 0.998) over a range of 0.1-10 μg mL -1 which covers the therapeutic range. Proposed methods are practical for quantitative analysis of bosentan in real samples, and could be used in routine clinical analyses.

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“…Hamidi et al. presented a chiral separation of carvedilol enantiomers in human plasma after liquid–liquid microextraction (LLME). Carvedilol is a nonselective β‐blocker/α1‐blocker indicated in the treatment of heart failure and hypertension as a racemic mixture and each enantiomer exert different pharmacological responses.…”

Section: Concentration Adjustment (Kohlrausch Stacking)mentioning

confidence: 99%

“…Buzatto et al [23] reported a method for the assessment of nucleosides (putative tumor biomarkers) in blood serum and applied it to verify the effect of characteristics such as age, smoking habits, and alcohol consumption on nucleoside concentrations. Hamidi et al [24] presented a chiral separation of carvedilol enantiomers in human plasma after liquid-liquid microextraction (LLME). Carvedilol is a nonselective ␤-blocker/␣1-blocker indicated in the treatment of heart failure and hypertension as a racemic mixture and each enantiomer exert different pharmacological responses.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

et al. 2016

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The term "sample stacking" comprises a relatively broad spectrum of techniques that already form an almost inherent part of the methodology of CZE. Their principles are different but the effect is the same: concentration of a diluted analyte into a narrow zone and considerable increase of the method sensitivity. This review brings a survey of papers on electrophoretic sample stacking published approximately since the second quarter of 2014 till the first quarter of 2016. It is organized according to the principles of the stacking methods and includes chapters aimed at the concentration adjustment principle (Kohlrausch stacking), techniques based on pH changes, micellar methods, and other stacking techniques. Not reviewed are papers on transient ITP that are covered by another review in this issue.

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A Dispersive Liquid–Liquid Microextraction and Chiral Separation of Carvedilol in Human Plasma Using Capillary Electrophoresis

Abstract: The method was adapted for sensitive, selective and rapid determination of carvedilol enantiomers in human plasma samples.

Cited by 28 publications

References 40 publications

Mixed hemimicelle magnetic dispersive solid‐phase extraction using carbon‐coated magnetic nanoparticles for the determination of tramadol in urine samples

Mixed hemimicelle magnetic dispersive solid‐phase extraction using carbon‐coated magnetic nanoparticles for the determination of tramadol in urine samples

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

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