A disposable polymer lab-on-a-slide for point-of-care diagnostics of methicillin-resistant staphylococcus aureus (MRSA)

Abstract

This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.