A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Ramos, Edurne; Piñeiro, David; Soto, Manuel; Abánades, Daniel R.; Martín, M. Elena; Salinas, Matilde; González, Víctor

doi:10.1038/labinvest.3700535

Cited by 54 publications

(46 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA aptamers that specifically bind to the surface of spores of anthrax Bacillus anthracis (Bruno & Kiel, 1999; Bruno & Carrillo, 2012) and crystal-forming bacteria B. thuringiensis (Ikanovic et al, 2007) have been developed. DNA aptamers against Campylobacter jejuni (Bruno et al, 2009; Dwivedi et al, 2010), L. monocytogenes (Ohk et al, 2010; Suh & Jaykus, 2013), V. parahemolyticus (Duan et al, 2012a), Shigella dysenteriae (Duan et al, 2013a) , Streptococcus pyogenes (Hamula et al, 2011), Francisella tularensis (Vivekananda & Kiel, 2006), Pseudomonas aeruginosa (Wang et al, 2011), Leishmania infantum (Ramos et al, 2007), and Lactobacillus acidophilus (Hamula et al, 2008) have also been described. All these aptamers can potentially be used as a prospective therapeutic agents and diagnostic tools.…”

Section: Antibacterial Aptamersmentioning

confidence: 99%

Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

View full text Add to dashboard Cite

An important current issue of modern molecular medicine and biotechnology is the search for new approaches to early diagnostic assays and adequate therapy of infectious diseases. One of the promising solutions to this problem might be a development of nucleic acid aptamers capable of interacting specifically with bacteria, protozoa, and viruses. Such aptamers can be used for the specific recognition of infectious agents as well as for blocking of their functions. The present review summarizes various modern SELEX techniques used in this field, and of several currently identified aptamers against viral particles and unicellular organisms, and their applications. The prospects of applying nucleic acid aptamers for the development of novel detection systems and antibacterial and antiviral drugs are discussed.

show abstract

Section: Antibacterial Aptamersmentioning

confidence: 99%

Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

View full text Add to dashboard Cite

show abstract

“…Digoxigenin also has been used to label aptamers for use in biological assays (Ramos et al, 2007, 2010). It is a steroid with a low molecular weight of 390.51 Da that is found exclusively in the flowers and leaves of plants such as Digitalis purpuea , Digitalis orientalis , and Digitalis lanata .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, labeling of aptamers with biotin to produce complexes with avidin, streptavidin, or neutravidin in various cross-linked forms has been commonly employed when an aptamer is to be applied to multiple assays (Murphy et al, 2003; Baldrich et al, 2005; Li et al, 2009; Tanaka et al, 2009). Additionally, aptamers have been labeled with digoxigenin to produce complexes with anti-digoxigenin antibodies (Ramos et al, 2007, 2010). …”

Section: Introductionmentioning

confidence: 99%

Cotinine-conjugated aptamer/anti-cotinine antibody complexes as a novel affinity unit for use in biological assays

2012

View full text Add to dashboard Cite

Aptamers are synthetic, relatively short (e.g., 20-80 bases) RNA or ssDNA oligonucleotides that can bind targets with high affinity and specificity, similar to antibodies, because they can fold into unique, three-dimensional shapes. For use in various assays and experiments, aptamers have been conjugated with biotin or digoxigenin to form complexes with avidin or anti-digoxigenin antibodies, respectively. In this study, we developed a method to label the 5' ends of aptamers with cotinine, which allows formation of a stable complex with anti-cotinine antibodies for the purpose of providing another affinity unit for the application in biological assays using aptamers. To demonstrate the functionality of this affinity unit in biological assays, we utilized two well-known aptamers: AS1411, which binds nucleolin, and pegaptanib, which binds vascular endothelial growth factor. Cotinine-conjugated AS1411/anti-cotinine antibody complexes were successfully applied to immunoblot, immunoprecipitation, and flow cytometric analyses, and cotinine-conjugated pegaptanib/anti-cotinine antibody complexes were used successfully in enzyme immunoassays. Our results show that cotinine-conjugated aptamer/anti-cotinine antibody complexes are an effective alternative and complementary technique for aptamer use in multiple assays and experiments.

show abstract

“…Baseando-se nisso, foi produzida uma mistura de aptâmeros, que se ligam especificamente às regiões variáveis das histonas de L. infantum. Essa mistura, quando testada em ensaios imunoenzimáticos e em western blot, exibiu especificidade de ligação às histonas, apresentando uma aplicação em potencial desses aptâmeros no diagnóstico da leishmaniose 95,96 causada por L. infantum .…”

Section: Diagnóstico Sorológicounclassified

Diagnóstico da Leishmaniose Visceral Canina: grandes avanços tecnológicos e baixa aplicação prática

Faria

Andrade

2012

Rev Pan-Amaz Saude

View full text Add to dashboard Cite

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Cited by 54 publications

References 31 publications

Aptamers against pathogenic microorganisms

Aptamers against pathogenic microorganisms

Cotinine-conjugated aptamer/anti-cotinine antibody complexes as a novel affinity unit for use in biological assays

Diagnóstico da Leishmaniose Visceral Canina: grandes avanços tecnológicos e baixa aplicação prática

Contact Info

Product

Resources

About