A DNA Nanostructure‐based Biomolecular Probe Carrier Platform for Electrochemical Biosensing

Pei, Hao; Lü, Na; Wen, Yanli; Song, Shiping; Liu, Yan; Yan, Hao; Fan, Chunhai

doi:10.1002/adma.201002767

Cited by 507 publications

(503 citation statements)

References 42 publications

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“…This sensor could detect a concentration of 33 nM cocaine in a complex media, exceeding the sensitivity of previously reported aptamer-based sensors by more than two orders of magnitude. In another approach, Pei et al 58 replaced the DNA probe with an antithrombin aptamer at the top of the tetrahedron, which formed a thrombin sensor that could detect a concentration as low as 100 pM thrombin (Figure 5c). In addition, Ge et al 65 developed an electrochemical sensor for the genotyping of SNPs, and Bu et al 66 constructed a sensor for mercury ions using a mercury-specific oligonucleotide.…”

Section: D-structured Dna Probesmentioning

confidence: 99%

“…To this end, Pei et al 58 proposed a new concept to improve the probe-target recognition performance by introducing a 3D-structured DNA probe. As an example, a tetrahedron-shaped DNA nanostructure with a pendant DNA probe at one vertex and three thiol groups at the other three vertices was designed and used for the development of DNA sensors.…”

Section: D-structured Dna Probesmentioning

confidence: 99%

“…By using TSP-decorated Au electrodes, Pei et al 58 constructed an E-DNA sensor with a conventional sandwich detection strategy (Figure 5a). The electrochemical signal is produced via the substrate catalysis of HRP.…”

Section: D-structured Dna Probesmentioning

confidence: 99%

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Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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In addition to its fundamental function as a genetic code carrier, the utilization of DNA in various material applications has been actively explored over the past several decades. DNA is intrinsically an excellent type of self-assembly nanomaterial owing to its predictable base-pairing, high chemical stability and the convenience it possesses for synthesis and modification. Because of these unparalleled properties, DNA is widely used as excellent recognition elements in biosensors and as unique building blocks in nanodevices. A critical challenge in surface-based DNA biosensors lies in the reduced accessibility of target molecules to the DNA probes arranged on heterogeneous surfaces, especially when compared to probe-target recognition in homogeneous solutions. To improve the recognition abilities of these heterogeneous surface-confined DNA probes, much effort has been devoted to controlling the surface chemistry, conformation and packing density of the probe molecules, as well as the size and geometry of the surface. In this review, we aim to summarize the recent progress on the improvement of the probetarget recognition properties by introducing DNA nanostructure scaffolds. A range of new strategies have proven to provide a significantly enhanced range in the spatial positioning and the accessibility of the probes to the surface over previously reported linear structures. We will also describe the applications of DNA nanostructure scaffold-based biosensors. INTRODUCTIONDetection of nucleic acids (DNA or RNA) is an integral step in many applications, including in clinical diagnosis, environmental monitoring and antibioterrorism. 1 With these applications in mind, significant efforts have been made to develop sensitive, selective, rapid and cost-effective DNA (or RNA) biosensors. In parallel, DNAbased devices have also attracted a significant, recent interest owing to their promising applications in nanoelectronics, 2,3 biomolecular computations, 4-8 cellular imaging 9 and drug delivery. [10][11][12] In a typical design of DNA biosensors and nanodevices, oligonucleotides are often used as the molecular recognition layer. Because DNA is comprised of only four bases with A-T and G-C pairing, DNA hybridization processes are highly predictable and can be finely tuned with a rational design of the sequence. In addition, DNA oligonucleotides are usually easy to design, chemically stable and cost-effective.Understanding the physical structure of a DNA probe immobilized on a surface is critical in applications using DNA as a molecular recognition element. The properties of the DNA molecular recognition layer (such as selectivity, sensitivity and reproducibility) highly depend on the structure of the self-assembled DNA film. Modeling studies with thiolated DNA have demonstrated that efficient hybridization occurs in the well-controlled condition of surface-attached probes with large interprobe distances and upright conformations. [13][14][15][16][17][18][19][20][21][22][23][24]

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Section: D-structured Dna Probesmentioning

confidence: 99%

Section: D-structured Dna Probesmentioning

confidence: 99%

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Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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“…[79] Fan et al developed a DNA tetrahedron-based platform for the immobilization of biomolecular probes on a solid surface for sensing applications. [86] In this way, they realized a series of bio-sensing methods with significantly improved performances. In another work, they constructed supramolecular logic devices based on reconfigurable DNA tetrahedra and investigated the application of these structures towards in vivo sensing.…”

Section: In-vivo Cargo Deliverymentioning

confidence: 99%

Supramolecular Wireframe DNA Polyhedra: Assembly and Applications

Mao

Deng

2017

Chin. J. Chem.

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Wireframe, polyhedral, supramolecular complexes made of DNA have uniform sizes, defined three-dimensional shapes, porous facets, hollow interiors, good biocompatibilities, and chemical functionalizability. They confer great potentials in bottom-up nanoengineering towards various applications. In this review, we summarize recent advances in the rational design and programmed assembly of DNA wireframe polyhedra. Their assembly is based on three distinctively different strategies: individual strands-based assembly, tile-based assembly, and scaffolded DNA origami. Applications of these polyhedral structures in templated nanomaterial assembly and in-vivo cargo delivery are discussed. In the future, expanding the structural complexity and exploring their applications, especially in nanomaterials science and biomedicines, should be a primary focus of this rapidly developing and evolving activity of structural DNA nanotechnology.

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“…It is believed that exonuclease digestion-mediated AuNPs aggregation is the rate-limiting step because the hybridization between the AuNPs-attached CP probe and target is quick in homogenous solution. 278 Figure 49B). In contrast, reaction rate constant for the SM substrate was impossible to obtain due to a much lower Exo III activity.…”

Section: Kinetics Of Exo III Digestionmentioning

confidence: 99%

Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

Liang¹

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A DNA Nanostructure‐based Biomolecular Probe Carrier Platform for Electrochemical Biosensing

Cited by 507 publications

References 42 publications

Scaffolded biosensors with designed DNA nanostructures

Scaffolded biosensors with designed DNA nanostructures

Supramolecular Wireframe DNA Polyhedra: Assembly and Applications

Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

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