A DNAzyme‐Based Colorimetric Paper Sensor for Helicobacter pylori

Abstract

The reliable detection of pathogenic bacteria in complex biological samples using simple assays or devices remains am ajor challenge.H erein, we report as imple colorimetric paper device capable of providing specific and sensitive detection of Helicobacter pylori (H. pylori), apathogen strongly linked to gastric carcinoma, gastric ulcers,a nd duodenal ulcers,i ns tool samples.T he sensor molecule,a n RNA-cleaving DNAzyme obtained through in vitro selection, is activated by ap rotein biomarker from H. pylori. The colorimetric paper sensor,d esigned on the basis of the RNAcleaving property of the DNAzyme,i sc apable of sensitive detection of H. pylori in human stool samples with minimal sample processing and provides results in minutes.I tr emains fully functional under storage at ambient temperature for at least 130 days.T his work lays af oundation for developing DNAzyme-enabled paper-based point-of-care diagnostic devices for monitoring pathogens in complex samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

Helicobacter pylori detection methods in complex samples: a mini-review

“…1,2 It is considered to be the main cause of gastrointestinal cancers in human 1,2 HP attacks stomach epithelial cells by injecting its virulent factors. [3][4][5] HP infection can occur through human interaction with other host or with contaminated stool, water and food. 6,7 Up to date, the exact routes of HP infection transmission is still not fully investigated.…”

“…7 HP is showing an increasing resistance for the routine medical treatments by antibiotics. 3,[10][11][12][13] Thus, HP contamination detection is considerably important to prevent infections or at least diagnose the infection at early stages rather than just after serious disease and symptoms development. HP can cause several diseases, but not all hosts will suffer from symptoms as not all HP strains are seriously pathogenic.…”

“…3,[10][11][12][13] In addition, disease development also depends on host conditions like lifestyle and diet. 3,[10][11][12][13] Thus, the main challenge in HP treatment is to diagnose infection at early stage especially for hosts most likely to develop serious HP-related diseases and in host children. 10 Additionally, diagnosing HP medium contaminations within its susceptible transition routes like food, water and communal facilities will be very important to prevent infections.…”

“…11 Invasive methods, like histopathology staining and routine endoscopy, are typically oriented for detection in infected humans especially at late stages. 3,[10][11][12][13] However, non-invasive detection methods can be of in-vivo and in-vitro nature. 3,7,10,11 Urea Breath Test (UBT) is very common and widely used as in-vivo detection method of HP.…”

“…3,[10][11][12][13] However, non-invasive detection methods can be of in-vivo and in-vitro nature. 3,7,10,11 Urea Breath Test (UBT) is very common and widely used as in-vivo detection method of HP. 3,[10][11][12][13] Colorimetric sensors, Fluorescent-based sensors, Electrochemical sensors, ELISA kits as well as Lateral Flow Devices provide In-vitro detection of HP.…”