A domain containing the ESR motif in ENHANCER OF SHOOT REGENERATION 1 functions as a transactivation domain

Nomura, Yasuya; Matsuo, Naoki; Banno, Hiroharu

doi:10.5511/plantbiotechnology.26.395

Cited by 12 publications

(6 citation statements)

References 33 publications

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“…DRNL and its paralogue DORNRÖSCHEN (DRN), have highly redundant roles in embryonic patterning and cotyledon organogenesis (Chandler et al 2007). Both genes also have discrete roles, integrating differentially in directional auxin transport and CUC pathways during cotyledon initiation , both proteins possess a C-terminal activation domain (Matsuo and Banno 2008;Nomura et al 2009) and DNA binding has been shown for DRN ). This paper shows that DRNL transcription pre-patterns incipient floral primordia, with the number of expressing cells correlating to previous estimations of floral organ founder cells.…”

Section: Introductionmentioning

confidence: 94%

DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima

Chandler¹,

Jacobs²,

Cole³

et al. 2011

Plant Mol Biol

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Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutation of four canonical auxin response elements (AuxREs) in the DRNL promoter. DRNL expression identifies discrete modes of organ initiation in the four floral whorls, from individual or pairs of organ anlagen in the outer whorl of sepals to two morphogenetic fields pre-patterning petals and lateral stamens, or a ring-shaped field giving rise to the medial stamens before carpel primordia are specified. DRNL function only overlaps in the central stem cell zone with that of its paralogue, DORNRÖSCHEN (DRN). drnl mutants are affected in floral organ outgrowth, which functionally interplays with boundary specification as organ fusions are sensitized by loss of CUP-SHAPED COTYLEDON (CUC) gene activity, and synergistic interactions exist with mutants in local auxin biosynthesis and polar transport. DRNL apparently monitors and contributes to cellular decisions in the SAM and thus provides a novel molecular access to the interplay of founder cell specification, organ anlage and organogenesis in the SAM peripheral zone.

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Section: Introductionmentioning

confidence: 94%

DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima

Chandler¹,

Jacobs²,

Cole³

et al. 2011

Plant Mol Biol

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“…Thus, ERF12 and the combined functions of DRN, DRNL and PUCHI are opposing in terms of the repression and promotion of organ initiation, respectively. DRN, DRNL and PUCHI are considered to be transcriptional activators (see Chandler 2018) and all contain a transcriptionally active ESR domain (Nomura et al 2009). Conversely, ERF12 contains a C-terminal EAR-like repressor domain, which might transcriptionally repress downstream targets at the timepoint of incipient FM development within the IM, which is associated with large-scale gene downregulation (Wellmer et al 2006).…”

Section: Erf12 Interacts Genetically With Closely Related Erf Factorsmentioning

confidence: 99%

A phylogenetically conserved APETALA2/ETHYLENE RESPONSE FACTOR, ERF12, regulates Arabidopsis floral development

Chandler

Werr

2019

Plant Mol Biol

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Key message Arabidopsis ETHYLENE RESPONSE FACTOR12 (ERF12), the rice MULTIFLORET SPIKELET1 orthologue pleiotropically affects meristem identity, floral phyllotaxy and organ initiation and is conserved among angiosperms. Abstract Reproductive development necessitates the coordinated regulation of meristem identity and maturation and lateral organ initiation via positive and negative regulators and network integrators. We have identified ETHYLENE RESPONSE FACTOR12 (ERF12) as the Arabidopsis orthologue of MULTIFLORET SPIKELET1 (MFS1) in rice. Loss of ERF12 function pleiotropically affects reproductive development, including defective floral phyllotaxy and increased floral organ merosity, especially supernumerary sepals, at incomplete penetrance in the first-formed flowers. Wildtype floral organ number in early formed flowers is labile, demonstrating that floral meristem maturation involves the stabilisation of positional information for organogenesis, as well as appropriate identity. A subset of erf12 phenotypes partly defines a narrow developmental time window, suggesting that ERF12 functions heterochronically to fine-tune stochastic variation in wild type floral number and similar to MFS1, promotes meristem identity. ERF12 expression encircles incipient floral primordia in the inflorescence meristem periphery and is strong throughout the floral meristem and intersepal regions. ERF12 is a putative transcriptional repressor and genetically opposes the function of its relatives DORNRÖSCHEN, DORNRÖSCHEN-LIKE and PUCHI and converges with the APETALA2 pathway. Phylogenetic analysis suggests that ERF12 is conserved among all eudicots and appeared in angiosperm evolution concomitant with the generation of floral diversity.

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“…ESR1 encodes a TF belonging to the ERF family. The region between the AP2/ERF domain and the ESR motif in ESR1 is indicated to be essential for enhancement of shoot regeneration [ 30 ]. ESR1 acts as a transcriptional activator [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of the GmESR1 gene associated with soybean regeneration

Zhang

et al. 2017

PLoS ONE

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Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean.

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A domain containing the ESR motif in ENHANCER OF SHOOT REGENERATION 1 functions as a transactivation domain

Cited by 12 publications

References 33 publications

DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima

DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima

A phylogenetically conserved APETALA2/ETHYLENE RESPONSE FACTOR, ERF12, regulates Arabidopsis floral development

Functional analysis of the GmESR1 gene associated with soybean regeneration

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