A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human parastrongyliasis

Eamsobhana, Praphathip; Yoolek, Adisak; Punthuprapasa, Paibulaya; Suvouttho, Surasak

doi:10.1079/joh2004257

Cited by 21 publications

(16 citation statements)

References 19 publications

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“…(Solomon et al 2006). Most serological diagnostic studies for these two parasites had very high specificity, but used normal subjects as controls , Eamsobhana et al 2001, Maleewong et al 2001, Anantaphruti et al 2005, Laummaunwai et al 2007, Intapan et al 2010. We herein confirm that the 21 or 24-kDa antigenic diagnostic band for gnathostomiasis and the 29-kDa antigenic diagnostic band for angiostrongyliasis in EOM are highly specific.…”

Section: Resultssupporting

confidence: 71%

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Specificity of immunoblotting analyses in eosinophilic meningitis

Sawanyawisuth¹,

Sawanyawisuth

Intapan

et al. 2011

Mem. Inst. Oswaldo Cruz

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Angiostrongylus cantonensis and Gnathostoma spinigerum are the two most common causative parasites of eosinophilic meningitis (EOM). Serological tests are helpful tools for confirming the identity of the pathogen. Recent reports determined the specificity of such tests by using normal healthy controls. There have been limited studies done to rule out the cross-reactivity between these two causative parasites of EOM. This study aims to assess the specificity of the serological test in EOM by using each condition as a control for the other. Thirty-three patients with a diagnosis of EOM were enrolled. Sera from 22 patients with a positive 29-kDa antigenic diagnostic band of A. cantonensis were tested for the 21 and 24-kDa antigenic bands of G. spinigerum. Similarly, sera of 11 gnathostomiasis patients were tested for the 29-kDa diagnostic band for A. cantonensis. Only one patient in the angiostrongyliasis group had a positive result for the 21 and 24-kDa antigenic bands of G. spinigerum, while no gnathostomiasis patients showed a positive result for the 29-kDa antigenic band of A. cantonensis. The specificity of the 21 and 24-kDa antigenic bands for gnathostomiasis and the 29-kDa antigenic band for A. cantonensis was 95.5% and 100%, respectively. The antigenic bands for the diagnosis of gnathostomiasis and angiostrongyliasis in EOM were highly specific

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Section: Resultssupporting

confidence: 71%

“…Most studies of human EOM had normal healthy subjects or various helminthically infected subjects as controls , Eamsobhana et al 2001, Maleewong et al 2001, Anantaphruti et al 2005, Laummaunwai et al 2007, Intapan et al 2010. There are limited studies ruling out cross-reactivity between the two causative parasites of EOM.…”

mentioning

confidence: 99%

Specificity of immunoblotting analyses in eosinophilic meningitis

Sawanyawisuth¹,

Sawanyawisuth

Intapan

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…Although computed tomography imaging can improve diagnostic sensitivity [18], its accessibility is problematic for individuals in remote areas and those living in poor economic conditions. Because immunoserological diagnosis has high sensitivity and specificity [19], the use of simple immunodiagnostic methods such as dot blot ELISA [20] or immunochromatography test kits [21] is recommended in the endemic regions. In the present case, unfortunately our mobile clinic did not carry such an immunodiagnostic kit for paragonimiasis.…”

Section: Discussionmentioning

confidence: 99%

Asymptomatic Human Paragonimiasis among the Karen People in Tak Province, Thailand: A Case Report

et al. 2020

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During the mobile clinic activities in Tak Province, Thailand, Paragonimus sp. eggs were found in a fecal sample of a 72-year-old Karen resident. Paragonimus DNA was amplified from the stool sample and identified to P. heterotremus. The patient did not have any symptoms. Apparent pulmonary lesion was not found on the chest X-ray. The patient admitted habitual consumption of semi-cooked or roasted waterfall crabs for several years. The waterfall crabs collected from stream near the village were found negative for Paragonimus metacercariae. In northern Thailand, paragonimiasis remains as one of the public health concerns and should be ruled out for asymptomatic pulmonary patients.

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“…16 Antigens at molecular weights of around 29-31 kDa, extracted from young adult and adult female A. cantonensis have often been reported as immunodiagnostic targets for human angiostrongyliasis. [17][18][19][20] The 31-kDa protein has been used for immunodiagnosis of angiostronyliasis in regional hospitals in Thailand. 21 However, extraction of the protein is laborious and results in a low yield of purified antigen.…”

Section: Introductionmentioning

confidence: 99%

Application of Recombinant Angiostrongylus cantonensis Galectin-2 Protein for Serodiagnosis of Human Angiostrongyliasis by Immunoblotting

Somboonpatarakun

Intapan

Sanpool

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Angiostrongyliasis is a foodborne disease caused by a zoonotic nematode, Angiostrongylus cantonensis, which produces eosinophilic meningitis or meningoencephalitis (EOM) in humans. Definitive diagnosis is rarely possible because worms are almost never recovered from patients. Human disease can be diagnosed by clinical symptoms and serological tests. Presently, diagnosis is performed by serological detection of antibodies against specific somatic antigens (molecular mass 29-31 kDa) extracted from female worms. The life cycle of A. cantonensis must be maintained in the laboratory to provide a source of this diagnostic antigen. Here, we cloned and expressed recombinant A. cantonensis galectin-2 (rAcGal2) corresponding to a 31-kDa antigenic peptide. Recombinant protein was purified and used in immunoblot tests, which showed reactions with human serum panels consisting of six confirmed angiostrongyliasis and 24 clinically diagnosed cases of EOM-associated with angiostrongyliasis, 160 samples from patients with other parasitic infections, and 30 samples from normal healthy subjects. Accuracy, sensitivity, specificity, and positive and negative predictive values were 95.0%, 93.3%, 95.3%, 75.7%, and 98.9%, respectively. The test was nonreactive with sera of human gnathostomiasis and cysticercosis, two diseases that could present similar neurological symptoms. Recombinant AcGal2 has potential as a diagnostic antigen and could replace native parasite antigens in further development of an angiostrongyliasis serodiagnostic test kit.

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A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human parastrongyliasis

Cited by 21 publications

References 19 publications

Specificity of immunoblotting analyses in eosinophilic meningitis

Specificity of immunoblotting analyses in eosinophilic meningitis

Asymptomatic Human Paragonimiasis among the Karen People in Tak Province, Thailand: A Case Report

Application of Recombinant Angiostrongylus cantonensis Galectin-2 Protein for Serodiagnosis of Human Angiostrongyliasis by Immunoblotting

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