A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model

Luo, Yuzi; Terkawi, Mohamad Alaa; Jia, Honglin; Aboge, Gabriel Oluga; Goo, Youn‐Kyoung; Cao, Shinuo; Li, Yan; Yu, Liang; Ooka, Hideo; Kamyingkird, Ketsarin; Masatani, Tatsunori; Shou-fa, Zhang; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan

doi:10.1016/j.exppara.2011.10.012

Cited by 31 publications

(28 citation statements)

References 34 publications

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“…Cross-reacting antibody responses to B. microti antigens have not been reported for sera from other humans and animals infected with Babesia species, and our results for sera from B. duncani-and B. divergens-infected patients support those findings (14,17,18,36,37,41,47). Given the apparent species specificity of the response, one or more antigens from both B. duncani and B. divergens would be required to cover all of the potential Babesia infections that might be found in the U.S. blood supply.…”

Section: Discussionsupporting

confidence: 82%

“…Hamster antibodies to BmSA1 were apparent by day 4 to 5 of experimental B. microti infection, and an indirect ELISA using recombinant BmSA1 as the antigen was 100% sensitive compared to blood film microscopy (36,37). Sera from pathogen-free animals or from animals infected with pathogens other than B. microti (including other Babesia spp.)…”

Section: Discussionmentioning

confidence: 99%

“…Sera from pathogen-free animals or from animals infected with pathogens other than B. microti (including other Babesia spp.) were uniformly negative by indirect ELISA (36,37). Data from human serum assays using this antigen, however, are limited (35).…”

Section: Discussionmentioning

confidence: 99%

“…BmSA1 shared 75% of its coding sequence with a clone previously identified as BMN1-9 by Lodes et al (35). BmSA1 antigen appeared to be secreted into the serum of the host and was not recognized by sera from animals infected with B. bovis, B. bigemina, B. equi, or B. gibsoni (37).…”

mentioning

confidence: 98%

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Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens

Priest

Moss

Won

et al. 2012

Clin Vaccine Immunol

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Human babesiosis, a blood-borne infection caused by several species of Babesia, including B. microti, is an emerging disease that is endemic in the Northeast, upper Midwest, and Pacific Northwest regions of the United States. Risk factors for babesiosis include exposure to the infected tick vector and blood transfusions from infected donors. In this work, we cloned and expressed two of the immunodominant antigens from B. microti and used them in a multiplex bead format assay (MBA) to detect parasitespecific IgG responses in human sera. The MBA using recombinant B. microti secreted antigen 1 (BmSA1) protein was more specific (100%) and slightly more sensitive (98.7%) than the assay using a truncated recombinant BMN1-17 construct (97.6% and 97.4%, respectively). Although some antibody reactivity was observed among sera from confirmed-malaria patients, only one Plasmodium falciparum sample was simultaneously positive for IgG antibodies to both antigens. Neither antigen reacted with sera from babesiosis patients who were infected with Babesia species other than B. microti. Both positive and negative MBA results were reproducible between assays and between instruments. Additional studies of these recombinant antigens and of the multiplex bead assay using blood samples from clinically defined babesiosis patients and from blood donors are needed to more clearly define their usefulness as a blood screening assay.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

See 2 more Smart Citations

Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens

Priest

Moss

Won

et al. 2012

Clin Vaccine Immunol

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“…The dilution of the capture antibody, detection antibody, test samples and goat anti-mouse IgG-HRP conjugate (#ab97023; Abcam, Shanghai, China) were optimized by checkerboard titration. Sandwich ELISA was performed following the method described in literature (15). Anti-HAAH-C mAb was diluted in a coating buffer (0.05 M carbonate-bicarbonate buffer, pH 9.6) and coated microtiter plates at 4˚C overnight.…”

Section: Double Antibody Sandwich-elisamentioning

confidence: 99%

Development of a novel anti-human aspartyl-(asparaginyl) β-hydroxylase monoclonal antibody with diagnostic and therapeutic potential

Huyan

Dong

et al. 2017

Oncology Letters

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Abstract. Human aspartyl-(asparaginyl)-β-hydroxylase (HAAH) has recently been the subject of several studies, as it was previously observed to be overexpressed in numerous types of carcinoma cells and tissues in patient tumor samples. HAAH has been implicated in tumor invasion and metastasis, indicating that it may be an important target and biomarker for tumor diagnosis and treatment. However, the immunological tools currently available for the study of this protein, including monoclonal antibodies, are limited, as is the present knowledge regarding the role of HAAH in tumor therapy and diagnosis. In the present study, a recombinant C-terminal domain of HAAH was expressed in Pichia pastoris and a novel monoclonal antibody (mAb) targeting HAAH (HAAH-C) was constructed. Immunofluorescence and antibody-dependent cellular cytotoxicity (ADCC) assays were used to demonstrate the specificity and ADCC activity of this antibody. The results demonstrated that this anti-C-terminal HAAH mAB, in combination with an existing anti-N terminal HAAH mAb, exhibited a high response to native HAAH from carcinoma cell culture supernatant, as measured with a double antibody sandwich enzyme-linked immunosorbent assay. This validated novel mAB-HAAH-C may prompt further studies into the underlying mechanisms of HAAH, and the exploration of its potential in tumor diagnosis and therapy.

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Babesia Species

Gelfand¹,

Vannier²

2015

Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases

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A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model

Cited by 31 publications

References 34 publications

Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens

Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens

Development of a novel anti-human aspartyl-(asparaginyl) β-hydroxylase monoclonal antibody with diagnostic and therapeutic potential

Babesia Species

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