A double-recognized aptamer-molecularly imprinted monolithic column for high-specificity recognition of ochratoxin A

Lyu, Haixia; Sun, Haoran; Zhu, Yimen; Wang, Jun; Xie, Zenghong; Li, Jinxia

doi:10.1016/j.aca.2019.12.052

Cited by 36 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensitive OTA aptasensor introduced in this work displayed rapidity, low cost, easy preparation, and good specificity with satisfied reproducibility, which could be potentially employed for detection of OTA in real samples as a portable and reliable tool. To be noted, the interference of OTB should be eliminated or reduced by developing on-column specific recognition of OTA with aptamer-based hybrid affinity monolithic column coupled with HPLC or LC for better application [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor

Alhamoud

Zhou

et al. 2021

Biosensors

View full text Add to dashboard Cite

Ochratoxin A (OTA) primarily obtained by the genera aspergillus and penicillium, is one of the toxic substances for different organs and systems of the human body such as the kidney, liver, neurons and the immune system. Moreover, it is considered to cause tumors and fetal malformation even at a very low concentration. Fast and sensitive assay for detection of OTA at ultralow levels in foods and agricultural products has been an increasing demand. In this study, a new label-free electrochemical biosensor based on three-dimensional reduced graphene oxide/gold nanoparticles/aptamer for OTA detection was constructed. The 3D-rGO/Au NPs nanocomposites were firstly synthesized using a one-pot hydrothermal process under optimized experimental conditions. The 3D-rGO/Au NPs with considerable particular surface area and outstanding electrical conductivity was then coated on a glass carbon electrode to provide tremendous binding sites for -SH modified aptamer via the distinctive Au–S linkage. The presence of OTA was specifically captured by aptamer and resulted in electrochemical impedance spectroscopy (EIS) signal response accordingly. The constructed impedimetric aptasensor obtained a broad linear response from 1 pg/mL to 10 ng/mL with an LOD of 0.34 pg/mL toward OTA detection, highlighting the excellent sensitivity. Satisfactory reproducibility was also achieved with the relative standard deviation (RSD) of 1.393%. Moreover, the proposed aptasensor obtained a good recovery of OTA detection in red wine samples within the range of 93.14 to 112.75% along with a low LOD of 0.023 ng/mL, indicating its applicability for OTA detection in real samples along with economical, specific, susceptible, fast, easy, and transportable merits.

show abstract

Section: Discussionmentioning

confidence: 99%

Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor

Alhamoud

Zhou

et al. 2021

Biosensors

View full text Add to dashboard Cite

show abstract

“…The type of material that is created by this process is known as a molecularly imprinted polymer (MIP) [201–204]. This method has been used in AMC with an imprint of protein G to capture a recombinant form of this protein from cell lysates, with an imprint of cytochrome c to recognize cytochrome c versus lysozyme, and in a hybrid aptamer/MIP monolith for the analysis of ochratoxin A in beer [201,205,206].…”

Section: Immobilization Methods For Affinity Monolithsmentioning

confidence: 99%

“…Advantages of using aptamers are their good selectivity, ability to be quickly regenerated, relatively low production cost, ease of modification, and good chemical or thermal stability [306]. Aptamers have been immobilized on both organic and hybrid monoliths [180,186,206,307–313]. Several studies have demonstrated the capture of thrombin by anti‐thrombin aptamers attached to GMA/EDMA or hybrid monoliths [186,307,311].…”

Section: Applications Of Amcmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and recent developments

2021

View full text Add to dashboard Cite

Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target‐specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal‐ion affinity chromatography, and dye‐ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

show abstract

“…Both Apts and MIPs are applied to reduce non-specific adsorption in sample preparation. A doublerecognized aptamer-molecularly imprinted (Apt-MI) monolithic column for high-specificity recognition of ochratoxin A (OTA) was constructed [92]. The hydrogen-bond interactions between the amine groups of functional monomer (AMPS) and the chlorine atoms of OTA could increase specific adsorption of OTA on Apt-MI monolithic column.…”

Section: Monolithic Mipsmentioning

confidence: 99%

Strategies of molecular imprinting-based solid-phase extraction prior to chromatographic analysis

Arabi

Ostovan

Bagheri

et al. 2020

TrAC Trends in Analytical Chemistry

374

View full text Add to dashboard Cite

A double-recognized aptamer-molecularly imprinted monolithic column for high-specificity recognition of ochratoxin A

Cited by 36 publications

References 40 publications

Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor

Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor

Affinity monolith chromatography: A review of general principles and recent developments

Strategies of molecular imprinting-based solid-phase extraction prior to chromatographic analysis

Contact Info

Product

Resources

About