A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer

Abstract

Objective. Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods. Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples. The profiling results were also validated in vitro in three human GC cell lines (BGC-823, MGC-803, and GTL-16) and a normal gastric epithelial cell line (GES-1). Results. The miR sequencing approach detected 5 differentially expressed miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, and hsa-miR-30a-3p, which were significantly downmodulated between the tumoral and peritumoral GC tissues. Most of the results were further confirmed by qRT-PCR, while no change was observed for hsa-miR-30a-3p. The in vitro finding also agreed with the results of both miR sequencing and qRT-PCR for hsa-miR-204-5p, hsa-miR-155-5p, and hsa-miR-132-3p. Conclusion. Together, our findings may serve to identify new molecular alterations as well as to enrich the miR profiling in human GC.

miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer

“…miRNA-21 promotes the growth of gastric cancer cells by adjusting and controlling PEG2 [11]. Similarity, previous expression profiling data uncovered that as an onco-suppressors, miR-204-5p significantly downregulated in GC tissue [12][13][14]. It plays an important role in regulating metastasis, promoting cell apoptosis, and inhibiting cell proliferation [15][16][17].…”

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

“…MicroRNA‐155 has been found to be upregulated and act as a tumor promoter in cancer tissues and cell lines, including gastric cancer; however, its function and the relevant pathways concerned with TGFβR2 have not been sufficiently elucidated. In this study, an inverse correlation was found between miR‐155 and TGFβR2 after measuring the expression levels of miR‐155 and TGFβR2 in GC tissues and paired peritumoral gastric tissues.…”

miR‑204‑5p and miR‑3065‑5p exert antitumor effects on melanoma cells

“…miR-204-5p upregulation in glioma cells suppresses glioma cells growth, migration and invasion by triggering RAB22A, which belongs to RAS proteins superfamily ( 43 ). miR-204-5p was demonstrated to be downregulated in several malignancies including renal cell carcinoma and gastric cancer ( 44 , 45 ). Our results correspond to the aforementioned data that suggest that miR-204-5p functions as cancer suppressor in various malignancies, including melanoma, with the targeting of several genes implicated in carcinogenesis.…”